+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6j4i | ||||||
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タイトル | A conserved and buried edge-to-face aromatic interaction in SUMO is vital for the SUMO pathway | ||||||
要素 | Small ubiquitin-related modifier 1 | ||||||
キーワード | IMMUNE SYSTEM / Post-translational modifier | ||||||
機能・相同性 | 機能・相同性情報 protein localization to nuclear pore / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) / nuclear stress granule / PML body organization / SUMO is transferred from E1 to E2 (UBE2I, UBC9) ...protein localization to nuclear pore / negative regulation of transcription by transcription factor localization / SUMOylation of nuclear envelope proteins / Negative regulation of activity of TFAP2 (AP-2) family transcription factors / SUMO is proteolytically processed / negative regulation of delayed rectifier potassium channel activity / SUMO is conjugated to E1 (UBA2:SAE1) / nuclear stress granule / PML body organization / SUMO is transferred from E1 to E2 (UBE2I, UBC9) / negative regulation of action potential / small protein activating enzyme binding / regulation of calcium ion transmembrane transport / SUMOylation of DNA methylation proteins / SUMOylation of immune response proteins / XY body / SUMOylation of SUMOylation proteins / Maturation of nucleoprotein / SUMOylation of RNA binding proteins / regulation of cardiac muscle cell contraction / Postmitotic nuclear pore complex (NPC) reformation / Maturation of nucleoprotein / negative regulation of protein import into nucleus / SUMOylation of ubiquitinylation proteins / roof of mouth development / ubiquitin-specific protease binding / negative regulation of DNA binding / ubiquitin-like protein ligase binding / transcription factor binding / SUMOylation of DNA replication proteins / SUMOylation of transcription factors / protein sumoylation / potassium channel regulator activity / SUMOylation of DNA damage response and repair proteins / nuclear pore / Regulation of IFNG signaling / cellular response to cadmium ion / SUMOylation of chromatin organization proteins / SUMOylation of transcription cofactors / SUMOylation of intracellular receptors / positive regulation of protein-containing complex assembly / PKR-mediated signaling / negative regulation of DNA-binding transcription factor activity / PML body / Formation of Incision Complex in GG-NER / protein tag activity / regulation of protein localization / positive regulation of proteasomal ubiquitin-dependent protein catabolic process / Recruitment and ATM-mediated phosphorylation of repair and signaling proteins at DNA double strand breaks / cellular response to heat / nuclear membrane / nuclear body / protein stabilization / nuclear speck / DNA repair / negative regulation of DNA-templated transcription / ubiquitin protein ligase binding / nucleolus / enzyme binding / RNA binding / nucleoplasm / nucleus / plasma membrane / cytosol 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Chatterjee, K.S. / Das, R. | ||||||
資金援助 | インド, 1件
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引用 | ジャーナル: J.Biol.Chem. / 年: 2019 タイトル: A conserved and buried edge-to-face aromatic interaction in small ubiquitin-like modifier (SUMO) has a role in SUMO stability and function. 著者: Chatterjee, K.S. / Tripathi, V. / Das, R. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6j4i.cif.gz | 599.9 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6j4i.ent.gz | 503.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6j4i.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/j4/6j4i ftp://data.pdbj.org/pub/pdb/validation_reports/j4/6j4i | HTTPS FTP |
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-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 12389.860 Da / 分子数: 1 / 変異: F64L / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: SUMO1 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: P63165 |
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-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 | タイプ: solution 内容: 0.5 mM [U-98% 15N] Small Ubiquitin Like Modifier, 90% H2O/10% D2O Label: 15N_sample / 溶媒系: 90% H2O/10% D2O | ||||||||||||
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試料 |
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試料状態 | イオン強度: 172 mM / Label: conditions_1 / pH: 7.2 / 圧: 1 atm / 温度: 298 K |
-NMR測定
NMRスペクトロメーター | タイプ: Bruker AVANCE III / 製造業者: Bruker / モデル: AVANCE III / 磁場強度: 800 MHz |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 4 | |||||||||||||||
代表構造 | 選択基準: lowest energy | |||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 100 / 登録したコンフォーマーの数: 20 |