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- PDB-6ivu: Solution structure of the Sigma-anti-sigma factor complex RsgI1N-... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ivu | |||||||||||||||
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Title | Solution structure of the Sigma-anti-sigma factor complex RsgI1N-SigI1C from Clostridium thermocellum | |||||||||||||||
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![]() | TRANSCRIPTION / sigma factor | |||||||||||||||
Function / homology | ![]() DNA-templated transcription initiation => GO:0006352 / cellulose binding / sigma factor activity / carbohydrate metabolic process / DNA binding / metal ion binding / plasma membrane / cytoplasm Similarity search - Function | |||||||||||||||
Biological species | ![]() ![]() | |||||||||||||||
Method | SOLUTION NMR / simulated annealing | |||||||||||||||
![]() | Wei, Z. / Feng, Y. | |||||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Alternative sigma I/anti-sigma I factors represent a unique form of bacterial sigma /anti-sigma complex. Authors: Wei, Z. / Chen, C. / Liu, Y.J. / Dong, S. / Li, J. / Qi, K. / Liu, S. / Ding, X. / Ortiz de Ora, L. / Munoz-Gutierrez, I. / Li, Y. / Yao, H. / Lamed, R. / Bayer, E.A. / Cui, Q. / Feng, Y. | |||||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 1.1 MB | Display | ![]() |
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PDB format | ![]() | 939.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 558.4 KB | Display | ![]() |
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Full document | ![]() | 819 KB | Display | |
Data in XML | ![]() | 72.6 KB | Display | |
Data in CIF | ![]() | 96.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ivsC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 6170.172 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Strain: ATCC 27405 / Gene: Cthe_0059 / Cell line (production host): Rosseta (DE3) / Production host: ![]() ![]() |
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#2: Protein | Mass: 14226.666 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 1 mM [U-13C; U-15N] RsgI1N, 1 mM [U-13C; U-15N] SigI1C, 20 mM Bis-Tris, 50 mM sodium chloride, 2 mM EDTA, 90% H2O/10% D2O Label: sample1 / Solvent system: 90% H2O/10% D2O | ||||||||||||||||||||||||
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Sample |
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Sample conditions | Ionic strength: 50 mM / Label: condition1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 | |||||||||||||||
NMR representative | Selection criteria: lowest energy | |||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |