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- PDB-6iqq: Crystal structure of Prc with S452I and L252Y mutations in comple... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6iqq | ||||||
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Title | Crystal structure of Prc with S452I and L252Y mutations in complex with NlpI | ||||||
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![]() | HYDROLASE / protein quality control / peptidoglycan remodeling | ||||||
Function / homology | ![]() C-terminal processing peptidase / peptidoglycan metabolic process / cell outer membrane / protein catabolic process / protein-macromolecule adaptor activity / outer membrane-bounded periplasmic space / endopeptidase activity / receptor-mediated virion attachment to host cell / cell cycle / cell division ...C-terminal processing peptidase / peptidoglycan metabolic process / cell outer membrane / protein catabolic process / protein-macromolecule adaptor activity / outer membrane-bounded periplasmic space / endopeptidase activity / receptor-mediated virion attachment to host cell / cell cycle / cell division / response to antibiotic / serine-type endopeptidase activity / signal transduction / protein homodimerization activity / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Chueh, C.K. / Chang, C.I. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Structural Basis for the Differential Regulatory Roles of the PDZ Domain in C-Terminal Processing Proteases. Authors: Chueh, C.K. / Som, N. / Ke, L.C. / Ho, M.R. / Reddy, M. / Chang, C.I. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 366.8 KB | Display | ![]() |
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PDB format | ![]() | 296.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 469.2 KB | Display | ![]() |
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Full document | ![]() | 503.4 KB | Display | |
Data in XML | ![]() | 63.1 KB | Display | |
Data in CIF | ![]() | 86.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6iqrC ![]() 6iqsC ![]() 6iquC ![]() 5wqlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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Components
#1: Protein | Mass: 33841.562 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 77670.945 Da / Num. of mol.: 2 / Mutation: S452I, L252Y Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() Strain: K12 / Gene: prc, tsp, b1830, JW1819 / Production host: ![]() ![]() References: UniProt: P23865, C-terminal processing peptidase #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.95 Å3/Da / Density % sol: 58.25 % |
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Crystal grow | Temperature: 295 K / Method: vapor diffusion, hanging drop / pH: 8 / Details: PEG3350, Calcium acetate, Imidazole |
-Data collection
Diffraction | Mean temperature: 110 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: Oct 25, 2017 Details: LN2-Cooled Fixed-Exit Double Crystal Si(111) Monochromator , A Pair of K-B Focusing Mirrors |
Radiation | Monochromator: LN2-Cooled, Fixed-Exit Double Crystal Monochromator Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.99984 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→30 Å / Num. obs: 64129 / % possible obs: 98.3 % / Redundancy: 7.9 % / Biso Wilson estimate: 38.87 Å2 / Rmerge(I) obs: 0.102 / Rpim(I) all: 0.055 / Rrim(I) all: 0.154 / Χ2: 0.961 / Net I/σ(I): 13.92 |
Reflection shell | Resolution: 2.8→2.9 Å / Redundancy: 6.9 % / Rmerge(I) obs: 0.615 / Mean I/σ(I) obs: 3.3 / Num. unique obs: 6401 / CC1/2: 0.895 / Rpim(I) all: 0.263 / Rrim(I) all: 0.748 / Χ2: 0.86 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5WQL Resolution: 2.8→29.98 Å / Cor.coef. Fo:Fc: 0.916 / Cor.coef. Fo:Fc free: 0.861 / SU B: 14.126 / SU ML: 0.276 / Cross valid method: THROUGHOUT / ESU R: 1.634 / ESU R Free: 0.393 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 56.008 Å2
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Refinement step | Cycle: 1 / Resolution: 2.8→29.98 Å
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Refine LS restraints |
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