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Yorodumi- PDB-6im4: Structural basis of AimP signaling molecule recognition by AimR i... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6im4 | ||||||
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Title | Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages | ||||||
Components |
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Keywords | PEPTIDE BINDING PROTEIN / Receptor / VIRUS | ||||||
Function / homology | : / latency-replication decision / AimR transcriptional regulator Function and homology information | ||||||
Biological species | Bacillus phage SPbeta (virus) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.93 Å | ||||||
Authors | Ouyang, S.Y. | ||||||
Citation | Journal: Protein Cell / Year: 2019 Title: Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages. Authors: Zhen, X. / Zhou, H. / Ding, W. / Zhou, B. / Xu, X. / Perculija, V. / Chen, C.J. / Chang, M.X. / Choudhary, M.I. / Ouyang, S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6im4.cif.gz | 173.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6im4.ent.gz | 139.7 KB | Display | PDB format |
PDBx/mmJSON format | 6im4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6im4_validation.pdf.gz | 447.3 KB | Display | wwPDB validaton report |
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Full document | 6im4_full_validation.pdf.gz | 457.3 KB | Display | |
Data in XML | 6im4_validation.xml.gz | 31.5 KB | Display | |
Data in CIF | 6im4_validation.cif.gz | 44.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/im/6im4 ftp://data.pdbj.org/pub/pdb/validation_reports/im/6im4 | HTTPS FTP |
-Related structure data
Related structure data | 6ipxC 6im2 C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 45849.477 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus phage SPbeta (virus) / Gene: aimR, yopK / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: O64094 #2: Protein/peptide | Mass: 588.701 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) synthetic construct (others) #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.36 Å3/Da / Density % sol: 47.87 % |
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Crystal grow | Temperature: 291.5 K / Method: vapor diffusion, sitting drop / pH: 8.5 / Details: PEG 4000 magnesium acetate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U1 / Wavelength: 0.979183 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Dec 18, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.979183 Å / Relative weight: 1 |
Reflection | Resolution: 1.928→61.565 Å / Num. obs: 135748 / % possible obs: 96.73 % / Redundancy: 10 % / CC1/2: 0.999 / Rmerge(I) obs: 0.106 / Rsym value: 0.111 / Net I/σ(I): 2 |
Reflection shell | Resolution: 1.93→2.03 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.93→61.565 Å / SU ML: 0.3 / Cross valid method: NONE / σ(F): 1.35 / Phase error: 32.32
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.93→61.565 Å
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Refine LS restraints |
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LS refinement shell |
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