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- PDB-6ic4: Cryo-EM structure of the A. baumannii MLA complex at 8.7 A resolution -
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Open data
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Basic information
Entry | Database: PDB / ID: 6ic4 | ||||||
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Title | Cryo-EM structure of the A. baumannii MLA complex at 8.7 A resolution | ||||||
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![]() | PROTEIN TRANSPORT / Membrane protein Antibiotic resistance ABC transporter | ||||||
Function / homology | ![]() phospholipid transporter activity / ATP-binding cassette (ABC) transporter complex / membrane => GO:0016020 / ATP hydrolysis activity / ATP binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ELECTRON MICROSCOPY / single particle reconstruction / cryo EM / Resolution: 8.7 Å | ||||||
![]() | Bergeron, J.R. / Kollman, J.M. | ||||||
![]() | ![]() Title: The Mla system and glycerophospholipid transport to the outer membrane. Authors: Cassandra Kamischke / Junping Fan / Julien Bergeron / Hemantha D Kulasekara / Zachary D Dalebroux / Anika Burrell / Justin M Kollman / Samuel I Miller / ![]() ![]() Abstract: The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is ...The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets. | ||||||
History |
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Structure visualization
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Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 276 KB | Display | ![]() |
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PDB format | ![]() | 167.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 0007MC M: map data used to model this data C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Components
#1: Protein | Mass: 19916.736 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 27209.285 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ttg2B, mlaE, A7M79_03105, A7M90_14775, A7N09_05510, Aba7804_03380, Aba810CP_02080, APD06_03860, AZE33_17930, B4R90_07005, B9X95_19830, BGC29_10505, C2U32_12245, C3415_18770, CAS83_02490, CHQ89_ ...Gene: ttg2B, mlaE, A7M79_03105, A7M90_14775, A7N09_05510, Aba7804_03380, Aba810CP_02080, APD06_03860, AZE33_17930, B4R90_07005, B9X95_19830, BGC29_10505, C2U32_12245, C3415_18770, CAS83_02490, CHQ89_09020, CPI82_03200, DCD77_11945, IX87_12245, LV38_01098, SAMEA104305242_00211, SAMEA104305261_03368, SAMEA104305271_02119, SAMEA104305341_02652, SAMEA104305343_00596, SAMEA104305351_00191, SAMEA104305385_01589 Production host: ![]() ![]() #3: Protein | Mass: 25554.471 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: ttg2A, A7M79_03110, A7M90_14770, A7N09_05505, AB719_19875, Aba810CP_02075, B4R90_07000, B9X91_22285, BGC29_10510, BWP00_03605, C3415_18775, C7G90_12995, CBE85_06065, CEJ63_09375, CPI82_03205, ...Gene: ttg2A, A7M79_03110, A7M90_14770, A7N09_05505, AB719_19875, Aba810CP_02075, B4R90_07000, B9X91_22285, BGC29_10510, BWP00_03605, C3415_18775, C7G90_12995, CBE85_06065, CEJ63_09375, CPI82_03205, CSB70_3297, IX87_12250, NCTC13305_02842, SAMEA104305208_00091, SAMEA104305242_00212, SAMEA104305271_02118, SAMEA104305318_02181, SAMEA104305341_02651, SAMEA104305343_00597, SAMEA104305351_00192, SAMEA104305385_01588 Production host: ![]() ![]() #4: Protein | Mass: 10644.346 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: ELECTRON MICROSCOPY |
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EM experiment | Aggregation state: PARTICLE / 3D reconstruction method: single particle reconstruction |
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Sample preparation
Component | Name: MlaBDEF / Type: COMPLEX / Entity ID: all / Source: RECOMBINANT |
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Source (natural) | Organism: ![]() |
Source (recombinant) | Organism: ![]() ![]() |
Buffer solution | pH: 7 |
Specimen | Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES |
Vitrification | Cryogen name: ETHANE |
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Electron microscopy imaging
Experimental equipment | ![]() Model: Tecnai F20 / Image courtesy: FEI Company |
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Microscopy | Model: FEI TECNAI F20 |
Electron gun | Electron source: ![]() |
Electron lens | Mode: BRIGHT FIELD |
Image recording | Electron dose: 100 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k) |
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Processing
CTF correction | Type: PHASE FLIPPING AND AMPLITUDE CORRECTION |
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Symmetry | Point symmetry: C2 (2 fold cyclic) |
3D reconstruction | Resolution: 8.7 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 14000 / Symmetry type: POINT |