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- PDB-6ic4: Cryo-EM structure of the A. baumannii MLA complex at 8.7 A resolution -

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Basic information

Entry
Database: PDB / ID: 6ic4
TitleCryo-EM structure of the A. baumannii MLA complex at 8.7 A resolution
Components
  • ABC transporter ATP-binding protein
  • ABC transporter permease
  • Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
  • Ttg2E
KeywordsPROTEIN TRANSPORT / Membrane protein Antibiotic resistance ABC transporter / protein transport
Function / homologyABC transporter permease MalE / P-loop containing nucleoside triphosphate hydrolase / MlaD protein / Permease MlaE / ABC transporter / STAS domain superfamily / Probable ABC transporter ATP-binding protein MlaF/Mkl / AAA+ ATPase domain / ATP-binding cassette, ABC transporter-type domain profile. / ABC transport permease subunit MlaE, Proteobacteria ...ABC transporter permease MalE / P-loop containing nucleoside triphosphate hydrolase / MlaD protein / Permease MlaE / ABC transporter / STAS domain superfamily / Probable ABC transporter ATP-binding protein MlaF/Mkl / AAA+ ATPase domain / ATP-binding cassette, ABC transporter-type domain profile. / ABC transport permease subunit MlaE, Proteobacteria / ABC transporter-like / Mce/MlaD / STAS domain / STAS domain / ATP-binding cassette (ABC) transporter complex / ATPase activity / integral component of membrane / ATP binding / ABC transporter ATP-binding protein / Ttg2E / Toluene tolerance efflux transporter (ABC superfamily, PerI-bind) / ABC transporter permease
Function and homology information
Specimen sourceAcinetobacter baumannii (bacteria)
MethodELECTRON MICROSCOPY / single particle reconstruction / cryo EM / 8.7 Å resolution
AuthorsBergeron, J.R. / Kollman, J.M.
CitationJournal: Elife / Year: 2019
Title: The Mla system and glycerophospholipid transport to the outer membrane.
Authors: Cassandra Kamischke / Junping Fan / Julien Bergeron / Hemantha D Kulasekara / Zachary D Dalebroux / Anika Burrell / Justin M Kollman / Samuel I Miller
Abstract: The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is ...The outer membrane (OM) of Gram-negative bacteria serves as a selective permeability barrier that allows entry of essential nutrients while excluding toxic compounds, including antibiotics. The OM is asymmetric and contains an outer leaflet of lipopolysaccharides (LPS) or lipooligosaccharides (LOS) and an inner leaflet of glycerophospholipids (GPL). We screened transposon mutants and identified a number of mutants with OM defects, including an ABC transporter system homologous to the Mla system in We further show that this opportunistic, antibiotic-resistant pathogen uses this multicomponent protein complex and ATP hydrolysis at the inner membrane to promote GPL export to the OM. The broad conservation of the Mla system in Gram-negative bacteria suggests the system may play a conserved role in OM biogenesis. The importance of the Mla system to OM integrity and antibiotic sensitivity suggests that its components may serve as new antimicrobial therapeutic targets.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Dec 2, 2018 / Release: Jan 23, 2019

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Assembly

Deposited unit
A: Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
B: Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
C: Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
D: Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
E: Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
F: Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)
G: ABC transporter permease
H: ABC transporter permease
I: ABC transporter ATP-binding protein
J: ABC transporter ATP-binding protein
K: Ttg2E
L: Ttg2E


Theoretical massNumber of molelcules
Total (without water)246,31712
Polyers246,31712
Non-polymers00
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551
MethodPISA

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Components

#1: Protein/peptide
Toluene tolerance efflux transporter (ABC superfamily, PerI-bind)


Mass: 19916.736 Da / Num. of mol.: 6 / Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Gene: ttg2C, SAMEA104305283_00402 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A334XBW3
#2: Protein/peptide ABC transporter permease / Putative phospholipid ABC transporter permease protein MlaE / Toluene tolerance efflux ABC transporter


Mass: 27209.285 Da / Num. of mol.: 2 / Source: (gene. exp.) Acinetobacter baumannii (bacteria)
Gene: ttg2B, mlaE, A7M79_03105, A7M90_14775, A7N09_05510, Aba7804_03380, Aba810CP_02080, APD06_03860, AZE33_17930, B4R90_07005, B9X95_19830, BGC29_10505, C2U32_12245, C3415_18770, CAS83_02490, CHQ89_09020, CPI82_03200, DCD77_11945, IX87_12245, LV38_01098, SAMEA104305242_00211, SAMEA104305261_03368, SAMEA104305271_02119, SAMEA104305341_02652, SAMEA104305343_00596, SAMEA104305351_00191, SAMEA104305385_01589
Production host: Escherichia coli (E. coli) / References: UniProt: V5V9F4
#3: Protein/peptide ABC transporter ATP-binding protein / Phospholipid ABC transporter ATP-binding protein MlaF / Toluene tolerance efflux transporter (ABC superfamily / ATP-bind)


Mass: 25554.471 Da / Num. of mol.: 2 / Source: (gene. exp.) Acinetobacter baumannii (bacteria)
Gene: ttg2A, A7M79_03110, A7M90_14770, A7N09_05505, AB719_19875, Aba810CP_02075, B4R90_07000, B9X91_22285, BGC29_10510, BWP00_03605, C3415_18775, C7G90_12995, CBE85_06065, CEJ63_09375, CPI82_03205, CSB70_3297, IX87_12250, NCTC13305_02842, SAMEA104305208_00091, SAMEA104305242_00212, SAMEA104305271_02118, SAMEA104305318_02181, SAMEA104305341_02651, SAMEA104305343_00597, SAMEA104305351_00192, SAMEA104305385_01588
Production host: Escherichia coli (E. coli) / References: UniProt: A0A086HZU3
#4: Protein/peptide Ttg2E


Mass: 10644.346 Da / Num. of mol.: 2 / Source: (gene. exp.) Acinetobacter baumannii (bacteria) / Gene: SAMEA104305318_02185 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A333U4T6

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: single particle reconstruction

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Sample preparation

ComponentName: MlaBDEF / Type: COMPLEX / Entity ID: 1, 2, 3, 4 / Source: RECOMBINANT
Source (natural)Organism: Acinetobacter baumannii (bacteria)
Source (recombinant)Organism: Escherichia coli (E. coli)
Buffer solutionpH: 7
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Tecnai F20 / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TECNAI F20
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 200 kV / Illumination mode: SPOT SCAN
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 100 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
SymmetryPoint symmetry: C2
3D reconstructionResolution: 8.7 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 14000 / Symmetry type: POINT

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