温度: 293 K / 手法: counter-diffusion / pH: 7.5 詳細: protein solution at 5.5 mg/mL in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 5 mM MgCl2; reservoir solution: 30% (m/v) PEG3350, 200 mM ammonium chloride pH 6.6. Crystallization and crystallographic ...詳細: protein solution at 5.5 mg/mL in 50 mM Tris-HCl pH 7.5, 200 mM NaCl, 5 mM MgCl2; reservoir solution: 30% (m/v) PEG3350, 200 mM ammonium chloride pH 6.6. Crystallization and crystallographic analysis were performed using the ChipX microfluidic device. PH範囲: 6.6-7.5
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データ収集
回折
平均測定温度: 293 K / Ambient temp details: in situ / Serial crystal experiment: N
解像度: 2.536→45.397 Å / SU ML: 0.36 / 交差検証法: THROUGHOUT / σ(F): 1.34 / 位相誤差: 24.14 詳細: The structure was refined using data collected on five crystals at room temperature