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- PDB-6tvz: Structure of a psychrophilic CCA-adding enzyme crystallized in th... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6tvz | ||||||||||||
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Title | Structure of a psychrophilic CCA-adding enzyme crystallized in the XtalController device | ||||||||||||
![]() | CCA-adding enzyme | ||||||||||||
![]() | RNA BINDING PROTEIN / tRNA maturation / tRNA nucleotidyltransferase / psychrophilic enzyme | ||||||||||||
Function / homology | ![]() tRNA 3'-terminal CCA addition / nucleotidyltransferase activity / tRNA binding / nucleotide binding Similarity search - Function | ||||||||||||
Biological species | ![]() | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | de Wijn, R. / Rollet, K. / Coudray, L. / Hennig, O. / Betat, H. / Moerl, M. / Lorber, B. / Sauter, C. | ||||||||||||
Funding support | ![]()
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![]() | ![]() Title: Monitoring the Production of High Diffraction-Quality Crystals of Two Enzymes in Real Time Using In Situ Dynamic Light Scattering Authors: de Wijn, R. / Rollet, K. / Engilberge, S. / McEwen, A.G. / Hennig, O. / Betat, H. / Moerl, M. / Riobe, F. / Maury, O. / Girard, E. / Benas, P. / Lorber, B. / Sauter, C. | ||||||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 187.7 KB | Display | ![]() |
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PDB format | ![]() | 131.3 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 462.9 KB | Display | ![]() |
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Full document | ![]() | 464.9 KB | Display | |
Data in XML | ![]() | 15.8 KB | Display | |
Data in CIF | ![]() | 21.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6tvyC ![]() 6qy6S S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 48498.266 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() | ||||||||
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#2: Chemical | #3: Chemical | #4: Chemical | #5: Water | ChemComp-HOH / | Has ligand of interest | N | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.74 Å3/Da / Density % sol: 67.4 % / Description: elongated tetragonal bipyramid |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 4.5 Details: A 10 micoliter drop of CCA-adding enzyme stock solution at 5 mg/ml in 0.05 M HEPES-NaOH pH 7.5, 0.1 M NaCl was incubated in the XtalController instrument. The crystallization was triggered ...Details: A 10 micoliter drop of CCA-adding enzyme stock solution at 5 mg/ml in 0.05 M HEPES-NaOH pH 7.5, 0.1 M NaCl was incubated in the XtalController instrument. The crystallization was triggered by the gradual addition of crystallant solution (1 M diammonium phosphate, 0.1 M ammonium acetate pH 4.5). |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Apr 23, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9799 Å / Relative weight: 1 |
Reflection | Resolution: 2.28→47.2 Å / Num. obs: 34470 / % possible obs: 0.995 % / Redundancy: 6.9 % / Biso Wilson estimate: 44.72 Å2 / CC1/2: 1 / Rpim(I) all: 0.034 / Rrim(I) all: 0.086 / Net I/σ(I): 15.5 |
Reflection shell | Resolution: 2.28→2.42 Å / Redundancy: 7 % / Num. unique obs: 5385 / CC1/2: 0.76 / Rpim(I) all: 0.448 / Rrim(I) all: 1.26 / % possible all: 98.5 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6QY6 Resolution: 2.28→47.19 Å / SU ML: 0.4031 / Cross valid method: FREE R-VALUE / σ(F): 1.33 / Phase error: 29.9338
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 50.98 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.28→47.19 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 40.6403073594 Å / Origin y: 37.0329519812 Å / Origin z: 54.0238436045 Å
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Refinement TLS group | Selection details: all |