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Yorodumi- PDB-6hzr: Apo structure of Pseudomonas aeruginosa Penicillin-Binding Protein 3 -
+Open data
-Basic information
Entry | Database: PDB / ID: 6hzr | ||||||
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Title | Apo structure of Pseudomonas aeruginosa Penicillin-Binding Protein 3 | ||||||
Components | Peptidoglycan D,D-transpeptidase FtsI | ||||||
Keywords | PEPTIDE BINDING PROTEIN / penicillin-binding protein / peptidoglycan / Transpeptidase | ||||||
Function / homology | Function and homology information peptidoglycan glycosyltransferase activity / serine-type D-Ala-D-Ala carboxypeptidase / FtsZ-dependent cytokinesis / serine-type D-Ala-D-Ala carboxypeptidase activity / division septum assembly / penicillin binding / peptidoglycan biosynthetic process / cell wall organization / regulation of cell shape / proteolysis / plasma membrane Similarity search - Function | ||||||
Biological species | Pseudomonas aeruginosa PAO1 (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.19 Å | ||||||
Authors | Bellini, D. / Dowson, C.G. | ||||||
Funding support | United Kingdom, 1items
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Citation | Journal: J.Mol.Biol. / Year: 2019 Title: Novel and Improved Crystal Structures of H. influenzae, E. coli and P. aeruginosa Penicillin-Binding Protein 3 (PBP3) and N. gonorrhoeae PBP2: Toward a Better Understanding of beta-Lactam ...Title: Novel and Improved Crystal Structures of H. influenzae, E. coli and P. aeruginosa Penicillin-Binding Protein 3 (PBP3) and N. gonorrhoeae PBP2: Toward a Better Understanding of beta-Lactam Target-Mediated Resistance. Authors: Bellini, D. / Koekemoer, L. / Newman, H. / Dowson, C.G. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6hzr.cif.gz | 128.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6hzr.ent.gz | 94.4 KB | Display | PDB format |
PDBx/mmJSON format | 6hzr.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6hzr_validation.pdf.gz | 434.4 KB | Display | wwPDB validaton report |
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Full document | 6hzr_full_validation.pdf.gz | 446.9 KB | Display | |
Data in XML | 6hzr_validation.xml.gz | 27.8 KB | Display | |
Data in CIF | 6hzr_validation.cif.gz | 42.7 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/hz/6hzr ftp://data.pdbj.org/pub/pdb/validation_reports/hz/6hzr | HTTPS FTP |
-Related structure data
Related structure data | 6hzjC 6hzoC 6hzqC 6i1eC 6i1iC 6r3xC 6r40C 6r42C 3oc2S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 58027.047 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Pseudomonas aeruginosa PAO1 (bacteria) / Gene: ftsI, pbpB, PA4418 / Production host: Escherichia coli BL21(DE3) (bacteria) References: UniProt: G3XD46, serine-type D-Ala-D-Ala carboxypeptidase |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.11 Å3/Da / Density % sol: 41.6 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop Details: 25%(w/v) polyethylene glycol 3350, 0.1M Bis-Tris propane, 1%(w/v) protamine sulphate, pH 7.8 |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I04-1 / Wavelength: 0.98 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 21, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.19→82.14 Å / Num. obs: 120346 / % possible obs: 87.5 % / Redundancy: 4.7 % / Rrim(I) all: 0.054 / Net I/σ(I): 14.5 |
Reflection shell | Resolution: 1.19→1.21 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 3OC2 Resolution: 1.19→60.08 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.949 / SU B: 0.852 / SU ML: 0.037 / Cross valid method: FREE R-VALUE / ESU R: 0.059 / ESU R Free: 0.059 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.014 Å2
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Refinement step | Cycle: 1 / Resolution: 1.19→60.08 Å
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Refine LS restraints |
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