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Open data
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Basic information
Entry | Database: PDB / ID: 6hdu | ||||||
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Title | Crystal structure of human Rab38 in complex with GTP | ||||||
![]() | Ras-related protein Rab-38 | ||||||
![]() | SIGNALING PROTEIN / Rab small GTPase / membrane trafficking / cellular dynamics | ||||||
Function / homology | ![]() BLOC-2 complex binding / AP-3 adaptor complex binding / phagosome acidification / positive regulation of phosphatidylcholine biosynthetic process / AP-1 adaptor complex binding / endosome to melanosome transport / platelet dense granule organization / mitochondria-associated endoplasmic reticulum membrane contact site / melanosome assembly / positive regulation of melanin biosynthetic process ...BLOC-2 complex binding / AP-3 adaptor complex binding / phagosome acidification / positive regulation of phosphatidylcholine biosynthetic process / AP-1 adaptor complex binding / endosome to melanosome transport / platelet dense granule organization / mitochondria-associated endoplasmic reticulum membrane contact site / melanosome assembly / positive regulation of melanin biosynthetic process / GTP-dependent protein binding / melanosome membrane / RAB geranylgeranylation / melanosome organization / RAB GEFs exchange GTP for GDP on RABs / protein localization to membrane / positive regulation of protein localization to cell periphery / small GTPase-mediated signal transduction / vesicle-mediated transport / phagocytic vesicle / mitochondrion organization / intracellular protein transport / trans-Golgi network / phagocytic vesicle membrane / melanosome / protein transport / cell body / lysosome / early endosome / GTPase activity / GTP binding / perinuclear region of cytoplasm / endoplasmic reticulum / mitochondrion / membrane / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | McGrath, E. / Waschbusch, D. / Khan, A.R. | ||||||
Funding support | ![]()
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![]() | ![]() Title: LRRK2 binds to the Rab32 subfamily in a GTP-dependent mannerviaits armadillo domain. Authors: McGrath, E. / Waschbusch, D. / Baker, B.M. / Khan, A.R. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 170.7 KB | Display | ![]() |
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PDB format | ![]() | 134.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 35.8 KB | Display | |
Data in CIF | ![]() | 51.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6ff8SC ![]() 6hh2C S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 20891.076 Da / Num. of mol.: 4 / Mutation: Q69L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | ChemComp-GTP / #3: Chemical | ChemComp-MG / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.17 Å3/Da / Density % sol: 43.22 % |
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Crystal grow | Temperature: 288 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: Morpheus A9 10% PEG 20000 20% PEG 550 MME 0.02M divalent cations 0.1M bicine/Trizma base pH 8.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 9M / Detector: PIXEL / Date: Jan 31, 2018 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98 Å / Relative weight: 1 |
Reflection | Resolution: 1.79→49.28 Å / Num. obs: 66651 / % possible obs: 98.7 % / Redundancy: 6.8 % / Rmerge(I) obs: 0.075 / Rrim(I) all: 0.082 / Net I/σ(I): 12.9 |
Reflection shell | Resolution: 1.79→1.83 Å / Rmerge(I) obs: 0.652 / Rrim(I) all: 0.718 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6ff8 Resolution: 1.793→49.28 Å / SU ML: 0.18 / Cross valid method: FREE R-VALUE / σ(F): 1.84 / Phase error: 25.88
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.793→49.28 Å
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Refine LS restraints |
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LS refinement shell |
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