[English] 日本語
Yorodumi- PDB-6ff8: Crystal structure of uncomplexed Rab32 in the active GTP-bound st... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6ff8 | ||||||
---|---|---|---|---|---|---|---|
Title | Crystal structure of uncomplexed Rab32 in the active GTP-bound state at 2.13 Angstrom resolution | ||||||
Components | Ras-related protein Rab-32 | ||||||
Keywords | SIGNALING PROTEIN / small GTPase / Rab / GTP / vesicle trafficking / cell dynamics | ||||||
Function / homology | Function and homology information BLOC-2 complex binding / AP-3 adaptor complex binding / AP-1 adaptor complex binding / endosome to melanosome transport / phagosome maturation / mitochondria-associated endoplasmic reticulum membrane contact site / melanosome assembly / melanosome organization / melanosome membrane / GTP-dependent protein binding ...BLOC-2 complex binding / AP-3 adaptor complex binding / AP-1 adaptor complex binding / endosome to melanosome transport / phagosome maturation / mitochondria-associated endoplasmic reticulum membrane contact site / melanosome assembly / melanosome organization / melanosome membrane / GTP-dependent protein binding / RAB geranylgeranylation / RAB GEFs exchange GTP for GDP on RABs / protein localization to membrane / antigen processing and presentation / endomembrane system / phagocytic vesicle / vesicle-mediated transport / mitochondrion organization / intracellular protein transport / trans-Golgi network / phagocytic vesicle membrane / melanosome / mitochondrial outer membrane / early endosome / GTPase activity / GTP binding / endoplasmic reticulum / mitochondrion / membrane / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.13 Å | ||||||
Authors | Khan, A.R. / Kecman, T. | ||||||
Funding support | Ireland, 1items
| ||||||
Citation | Journal: Small GTPases / Year: 2019 Title: LRRK2 binds to the Rab32 subfamily in a GTP-dependent mannerviaits armadillo domain. Authors: McGrath, E. / Waschbusch, D. / Baker, B.M. / Khan, A.R. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6ff8.cif.gz | 155.9 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6ff8.ent.gz | 122 KB | Display | PDB format |
PDBx/mmJSON format | 6ff8.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ff/6ff8 ftp://data.pdbj.org/pub/pdb/validation_reports/ff/6ff8 | HTTPS FTP |
---|
-Related structure data
Related structure data | 6hduC 6hh2C 4cymS S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
2 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 20588.467 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Homo sapiens (human) / Gene: RAB32 / Production host: Escherichia coli (E. coli) / References: UniProt: Q13637 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.27 Å3/Da / Density % sol: 45.78 % |
---|---|
Crystal grow | Temperature: 293 K / Method: vapor diffusion Details: O.2M sodium potassium tartrate, 0.1M Bis-Tris propane pH 8.5, 20% w/v PEG 3350 |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 2 / Wavelength: 0.9801 Å |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Sep 16, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9801 Å / Relative weight: 1 |
Reflection | Resolution: 2.13→41.36 Å / Num. obs: 20447 / % possible obs: 99.3 % / Redundancy: 4.2 % / Net I/σ(I): 12.4 |
Reflection shell | Resolution: 2.13→2.19 Å / Redundancy: 4.2 % / Rmerge(I) obs: 0.891 / Mean I/σ(I) obs: 1.5 / Num. unique obs: 1646 / CC1/2: 0.588 / Rpim(I) all: 0.488 / Rrim(I) all: 1.02 / Rsym value: 0 / % possible all: 99.7 |
-Processing
Software |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 4cym Resolution: 2.13→41.352 Å / SU ML: 0.22 / Cross valid method: FREE R-VALUE / σ(F): 0 / Phase error: 22.49
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.13→41.352 Å
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell |
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
| |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement TLS group |
|