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Yorodumi- PDB-6h86: Rebuilt and re-refined PDB entry 4R3Q: Crystal structure of SYCE3 -
+Open data
-Basic information
Entry | Database: PDB / ID: 6h86 | |||||||||
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Title | Rebuilt and re-refined PDB entry 4R3Q: Crystal structure of SYCE3 | |||||||||
Components | Synaptonemal complex central element protein 3 | |||||||||
Keywords | STRUCTURAL PROTEIN / Meiosis / synaptonemal complex | |||||||||
Function / homology | Function and homology information positive regulation of developmental process / central element / positive regulation of reproductive process / synaptonemal complex assembly / reciprocal meiotic recombination / ectopic germ cell programmed cell death / chromosome / spermatogenesis / positive regulation of apoptotic process / cell division ...positive regulation of developmental process / central element / positive regulation of reproductive process / synaptonemal complex assembly / reciprocal meiotic recombination / ectopic germ cell programmed cell death / chromosome / spermatogenesis / positive regulation of apoptotic process / cell division / apoptotic process / nucleoplasm / nucleus Similarity search - Function | |||||||||
Biological species | Mus musculus (house mouse) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.901 Å | |||||||||
Authors | Davies, O.R. | |||||||||
Funding support | United Kingdom, 2items
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Citation | Journal: J.Biol.Chem. / Year: 2019 Title: A molecular model for self-assembly of the synaptonemal complex protein SYCE3. Authors: Dunne, O.M. / Davies, O.R. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6h86.cif.gz | 108.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6h86.ent.gz | 86.9 KB | Display | PDB format |
PDBx/mmJSON format | 6h86.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/h8/6h86 ftp://data.pdbj.org/pub/pdb/validation_reports/h8/6h86 | HTTPS FTP |
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-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Components on special symmetry positions |
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-Components
#1: Protein | Mass: 10480.013 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Syce3, Tseg2 / Production host: Escherichia coli (E. coli) / References: UniProt: B5KM66 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.79 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop Details: 0.1M Citric acid, 7% 2-propanol, 1% PEG 20000, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRF / Beamline: BL17U / Wavelength: 0.9796 Å |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 30, 2013 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9796 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 16498 / % possible obs: 96.89 % / Redundancy: 3.9 % / Rsym value: 0.059 / Net I/σ(I): 22 |
Reflection shell | Resolution: 1.9→1.93 Å / Rsym value: 0.486 |
-Processing
Software |
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Refinement | Resolution: 1.901→23.661 Å / SU ML: 0.2 / Cross valid method: FREE R-VALUE / σ(F): 2.01 / Phase error: 25.32
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.901→23.661 Å
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Refine LS restraints |
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LS refinement shell |
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Refinement TLS params. | Method: refined / Origin x: 31.5493 Å / Origin y: -11.0764 Å / Origin z: -0.2192 Å
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Refinement TLS group | Selection details: all |