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Open data
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Basic information
Entry | Database: PDB / ID: 4r3q | ||||||
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Title | Crystal structure of SYCE3 | ||||||
![]() | Synaptonemal complex central element protein 3 | ||||||
![]() | CELL CYCLE / coiled-coil / Synaptonemal Complex | ||||||
Function / homology | ![]() positive regulation of developmental process / central element / positive regulation of reproductive process / synaptonemal complex assembly / reciprocal meiotic recombination / ectopic germ cell programmed cell death / chromosome / spermatogenesis / positive regulation of apoptotic process / cell division ...positive regulation of developmental process / central element / positive regulation of reproductive process / synaptonemal complex assembly / reciprocal meiotic recombination / ectopic germ cell programmed cell death / chromosome / spermatogenesis / positive regulation of apoptotic process / cell division / apoptotic process / nucleoplasm / nucleus Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Lu, J. / Feng, J. / Zhou, W. / Yang, X. / Shen, Y. | ||||||
![]() | ![]() Title: Structural insight into the central element assembly of the synaptonemal complex Authors: Lu, J. / Gu, Y. / Feng, J. / Zhou, W. / Yang, X. / Shen, Y. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 72.7 KB | Display | ![]() |
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PDB format | ![]() | 55.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 428.5 KB | Display | ![]() |
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Full document | ![]() | 429.7 KB | Display | |
Data in XML | ![]() | 7.9 KB | Display | |
Data in CIF | ![]() | 10.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 10480.013 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.66 Å3/Da / Density % sol: 53.79 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, sitting drop / pH: 3.5 Details: 0.1M Citric acid, 7% 2-propanol, 1% PEG 20000, pH 3.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Jun 30, 2013 |
Radiation | Monochromator: Si 111 CHANNEL / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9796 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. all: 16498 / Num. obs: 15986 / % possible obs: 96.89 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Biso Wilson estimate: 33.16 Å2 |
Reflection shell | Resolution: 1.9→1.93 Å / % possible all: 93.94 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.72 Å / VDW probe radii: 1 Å / Solvent model: FLAT BULK SOLVENT MODEL / Bsol: 70.032 Å2 / ksol: 0.406 e/Å3 | |||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 98.21 Å2 / Biso mean: 45.65 Å2 / Biso min: 21.42 Å2
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Refinement step | Cycle: LAST / Resolution: 1.901→23.661 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 6
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Refinement TLS params. | Method: refined / Origin x: 31.2242 Å / Origin y: -10.5981 Å / Origin z: -0.0676 Å
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Refinement TLS group | Selection details: ALL |