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- PDB-6h5s: Cryo-EM map of in vitro assembled Measles virus N into nucleocaps... -

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Entry
Database: PDB / ID: 6h5s
TitleCryo-EM map of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to viral genomic 5-prime RNA hexamers.
Components
  • NucleocapsidCapsid
  • RNA (5'-R(*AP*CP*CP*AP*GP*A)-3')
KeywordsVIRAL PROTEIN / Measles / Nucleocapsid / RNA / helical
Function / homologyParamyxovirus nucleocapsid protein / Paramyxovirus nucleocapsid protein / helical viral capsid / viral nucleocapsid / host cell cytoplasm / structural molecule activity / RNA binding / Nucleocapsid
Function and homology information
Biological speciesMeasles morbillivirus
synthetic construct (others)
MethodELECTRON MICROSCOPY / helical reconstruction / cryo EM / Resolution: 3.3 Å
AuthorsDesfosses, A. / Milles, S. / Ringkjobing Jensen, M. / Guseva, S. / Colletier, J.P. / Maurin, D. / Schoehn, G. / Gutsche, I. / Ruigrok, R. / Blackledge, M.
Funding supportFrance , 4件
OrganizationGrant numberCountry
GRALANR-10-LABX-49-01France
FRISBIANR-10-INSB-05-02France
European CommissionEMBOCOFUND2012, GA-2012-600394
Fondation Recherche Medicale (FRM)ARF20160936266France
CitationJournal: Proc. Natl. Acad. Sci. U.S.A. / Year: 2019
Title: Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication.
Authors: Ambroise Desfosses / Sigrid Milles / Malene Ringkjøbing Jensen / Serafima Guseva / Jacques-Philippe Colletier / Damien Maurin / Guy Schoehn / Irina Gutsche / Rob W H Ruigrok / Martin Blackledge /
Abstract: Assembly of paramyxoviral nucleocapsids on the RNA genome is an essential step in the viral cycle. The structural basis of this process has remained obscure due to the inability to control ...Assembly of paramyxoviral nucleocapsids on the RNA genome is an essential step in the viral cycle. The structural basis of this process has remained obscure due to the inability to control encapsidation. We used a recently developed approach to assemble measles virus nucleocapsid-like particles on specific sequences of RNA hexamers (poly-Adenine and viral genomic 5') in vitro, and determined their cryoelectron microscopy maps to 3.3-Å resolution. The structures unambiguously determine 5' and 3' binding sites and thereby the binding-register of viral genomic RNA within nucleocapsids. This observation reveals that the 3' end of the genome is largely exposed in fully assembled measles nucleocapsids. In particular, the final three nucleotides of the genome are rendered accessible to the RNA-dependent RNA polymerase complex, possibly enabling efficient RNA processing. The structures also reveal local and global conformational changes in the nucleoprotein upon assembly, in particular involving helix α6 and helix α13 that form edges of the RNA binding groove. Disorder is observed in the bound RNA, localized at one of the two backbone conformational switch sites. The high-resolution structure allowed us to identify putative nucleobase interaction sites in the RNA-binding groove, whose impact on assembly kinetics was measured using real-time NMR. Mutation of one of these sites, R195, whose sidechain stabilizes both backbone and base of a bound nucleic acid, is thereby shown to be essential for nucleocapsid-like particle assembly.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Jul 25, 2018 / Release: Jun 12, 2019
RevisionDateData content typeProviderType
1.0Jun 12, 2019Structure modelrepositoryInitial release

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Assembly

Deposited unit
C: Nucleocapsid
E: RNA (5'-R(*AP*CP*CP*AP*GP*A)-3')


Theoretical massNumber of molelcules
Total (without water)48,3242
Polymers48,3242
Non-polymers00
Water0
1
C: Nucleocapsid
E: RNA (5'-R(*AP*CP*CP*AP*GP*A)-3')
x 15


Theoretical massNumber of molelcules
Total (without water)724,86130
Polymers724,86130
Non-polymers00
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z3
point symmetry operation12
MethodUCSF CHIMERA

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Components

#1: Protein/peptide Nucleocapsid / Capsid / Nucleocapsid protein


Mass: 46425.863 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Measles morbillivirus / Production host: Escherichia coli (E. coli) / References: UniProt: B8PZP0
#2: RNA chain RNA (5'-R(*AP*CP*CP*AP*GP*A)-3')


Mass: 1898.229 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) synthetic construct (others) / Production host: synthetic construct (others)

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: HELICAL ARRAY / 3D reconstruction method: helical reconstruction

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Sample preparation

Component

Type: COMPLEX

IDNameEntity IDParent-IDSource
1Cryo-EM map of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to viral genomic 5-prime RNA hexamers.1, 20MULTIPLE SOURCES
2Measles virus N11RECOMBINANT
35-prime RNA21RECOMBINANT
Molecular weightExperimental value: NO
Source (natural)
IDEntity assembly-IDOrganismNcbi tax-ID
22Measles virus11234
33synthetic construct (others)32630
Source (recombinant)
IDEntity assembly-IDOrganismNcbi tax-ID
22Escherichia coli (E. coli)562
33synthetic construct (others)32630
Buffer solutionpH: 6 / Details: 50 mM Na-phosphate pH 6, 150 mM NaCl, 2 mM DTT
SpecimenConc.: 1 mg/ml / Embedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationInstrument: FEI VITROBOT MARK IV / Cryogen name: ETHANE / Humidity: 100 % / Chamber temperature: 293.15 K

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Electron microscopy imaging

Experimental equipment
Model: Tecnai Polara / Image courtesy: FEI Company
MicroscopyModel: FEI POLARA 300
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELDBright-field microscopy
Image recordingElectron dose: 30 e/Å2 / Film or detector model: GATAN K2 SUMMIT (4k x 4k)

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Processing

CTF correctionType: PHASE FLIPPING AND AMPLITUDE CORRECTION
Helical symmertyAngular rotation/subunit: -29.15924186 ° / Axial rise/subunit: 3.934877693 Å / Axial symmetry: C1
3D reconstructionResolution: 3.3 Å / Resolution method: FSC 0.143 CUT-OFF / Num. of particles: 102353 / Symmetry type: HELICAL
RefinementHighest resolution: 3.3 Å

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