- PDB-6gy3: Crystal Structure of C. glutamicum AmtR bound to glnA operator DNA -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6gy3
Title
Crystal Structure of C. glutamicum AmtR bound to glnA operator DNA
Components
AmtR protein
DNA (5'-D(*GP*TP*CP*TP*AP*TP*AP*GP*AP*TP*CP*GP*AP*TP*AP*GP*AP*C)-3')
DNA (5'-D(*GP*TP*CP*TP*AP*TP*CP*GP*AP*TP*CP*TP*AP*TP*AP*GP*AP*C)-3')
Keywords
TRANSCRIPTION / transcription regulator / TetR family / nitrogen regulation
Function / homology
Bacterial regulatory proteins, tetR family / DNA-binding HTH domain, TetR-type / TetR-type HTH domain profile. / Homeobox-like domain superfamily / DNA binding / DNA / DNA (> 10) / AmtR protein / Repressor of the high-affinity (Methyl) ammonium uptake system
A: AmtR protein B: AmtR protein C: DNA (5'-D(*GP*TP*CP*TP*AP*TP*AP*GP*AP*TP*CP*GP*AP*TP*AP*GP*AP*C)-3') D: DNA (5'-D(*GP*TP*CP*TP*AP*TP*CP*GP*AP*TP*CP*TP*AP*TP*AP*GP*AP*C)-3')
Mass: 22327.311 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: first 14 residues cleaved off due to factor Xa protease treatment electron density allowed tracing of residues 19-220 in both protomers Source: (gene. exp.) Corynebacterium glutamicum (bacteria) / Gene: amtR / Plasmid: pMALc2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: H7C699, UniProt: Q79VH8*PLUS
#2: DNA chain
DNA (5'-D(*GP*TP*CP*TP*AP*TP*AP*GP*AP*TP*CP*GP*AP*TP*AP*GP*AP*C)-3')
Mass: 5539.616 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Corynebacterium glutamicum (bacteria)
#3: DNA chain
DNA (5'-D(*GP*TP*CP*TP*AP*TP*CP*GP*AP*TP*CP*TP*AP*TP*AP*GP*AP*C)-3')
Mass: 5490.578 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Corynebacterium glutamicum (bacteria)
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 2.86 Å3/Da / Density % sol: 57 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 0.2 M ammonium acetate, 0.1 M Tris-HCl, pH 8.5, 25% PEG 3350