+Open data
-Basic information
Entry | Database: PDB / ID: 6go4 | ||||||
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Title | TdT chimera (Loop1 of pol mu) - binary complex with ddCTP | ||||||
Components | DNA nucleotidylexotransferase,DNA-directed DNA/RNA polymerase mu,DNA nucleotidylexotransferase | ||||||
Keywords | DNA BINDING PROTEIN / NHEJ pathway / DNA bridging / DNA polymerase polX | ||||||
Function / homology | Function and homology information DNA nucleotidylexotransferase / DNA nucleotidylexotransferase activity / Nonhomologous End-Joining (NHEJ) / DNA modification / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / DNA metabolic process / response to ATP / somatic hypermutation of immunoglobulin genes / B cell differentiation / euchromatin ...DNA nucleotidylexotransferase / DNA nucleotidylexotransferase activity / Nonhomologous End-Joining (NHEJ) / DNA modification / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / DNA metabolic process / response to ATP / somatic hypermutation of immunoglobulin genes / B cell differentiation / euchromatin / nuclear matrix / double-strand break repair via nonhomologous end joining / DNA recombination / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / hydrolase activity / chromatin / DNA binding / nucleoplasm / nucleus / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | Mus musculus (house mouse) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.96 Å | ||||||
Authors | Loc'h, J. / Gerodimos, C.A. / Rosario, S. / Lieber, M.R. / Delarue, M. | ||||||
Funding support | France, 1items
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Citation | Journal: J.Biol.Chem. / Year: 2019 Title: Structural evidence for an intransbase selection mechanism involving Loop1 in polymerase mu at an NHEJ double-strand break junction. Authors: Loc'h, J. / Gerodimos, C.A. / Rosario, S. / Tekpinar, M. / Lieber, M.R. / Delarue, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6go4.cif.gz | 170 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6go4.ent.gz | 130.1 KB | Display | PDB format |
PDBx/mmJSON format | 6go4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6go4_validation.pdf.gz | 776.1 KB | Display | wwPDB validaton report |
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Full document | 6go4_full_validation.pdf.gz | 777.1 KB | Display | |
Data in XML | 6go4_validation.xml.gz | 18.6 KB | Display | |
Data in CIF | 6go4_validation.cif.gz | 28.6 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/go/6go4 ftp://data.pdbj.org/pub/pdb/validation_reports/go/6go4 | HTTPS FTP |
-Related structure data
Related structure data | 6go3C 6go5C 6go6C 6go7C 1jmsS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 45848.984 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: There is an insertion of one residue between loop1 of pol mu and TdT (Q394). This insertion explain the numbering shift with wt-TdT.,There is an insertion of one residue between loop1 of pol ...Details: There is an insertion of one residue between loop1 of pol mu and TdT (Q394). This insertion explain the numbering shift with wt-TdT.,There is an insertion of one residue between loop1 of pol mu and TdT (Q394). This insertion explain the numbering shift with wt-TdT.,There is an insertion of one residue between loop1 of pol mu and TdT (Q394). This insertion explain the numbering shift with wt-TdT. Source: (gene. exp.) Mus musculus (house mouse) / Gene: Dntt, Tdt, Polm, polmu / Production host: Escherichia coli (E. coli) References: UniProt: P09838, UniProt: Q9JIW4, DNA nucleotidylexotransferase, DNA-directed DNA polymerase |
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#2: Chemical | ChemComp-NA / |
#3: Chemical | ChemComp-MG / |
#4: Chemical | ChemComp-DCT / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.19 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 6 Details: 20-24% PEG 6000, 400-800 mM lithium chloride, 100 mM MES |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SOLEIL / Beamline: PROXIMA 1 / Wavelength: 0.9785 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Nov 24, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9785 Å / Relative weight: 1 |
Reflection | Resolution: 1.96→47.78 Å / Num. obs: 34181 / % possible obs: 99.7 % / Redundancy: 6.5 % / Biso Wilson estimate: 46.53 Å2 / CC1/2: 0.998 / Rmerge(I) obs: 0.101 / Net I/σ(I): 11.02 |
Reflection shell | Resolution: 1.96→2.07 Å / Rmerge(I) obs: 1.666 / Num. unique obs: 5333 / CC1/2: 0.498 / % possible all: 98.5 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1JMS Resolution: 1.96→47.71 Å / Cor.coef. Fo:Fc: 0.946 / Cor.coef. Fo:Fc free: 0.93 / SU R Cruickshank DPI: 0.158 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.175 / SU Rfree Blow DPI: 0.157 / SU Rfree Cruickshank DPI: 0.15
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Displacement parameters | Biso mean: 55.94 Å2
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Refine analyze | Luzzati coordinate error obs: 0.28 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: 1 / Resolution: 1.96→47.71 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.98→2.04 Å / Total num. of bins used: 17
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Refinement TLS params. | Method: refined / Origin x: -15.1829 Å / Origin y: 12.1982 Å / Origin z: -20.1836 Å
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Refinement TLS group | Selection details: { A|* } |