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Structure paper

TitleStructural evidence for an intransbase selection mechanism involving Loop1 in polymerase mu at an NHEJ double-strand break junction.
Journal, issue, pagesJ. Biol. Chem., Vol. 294, Page 10579-10595, Year 2019
Publish dateJun 1, 2018 (structure data deposition date)
AuthorsLoc'h, J. / Gerodimos, C.A. / Rosario, S. / Tekpinar, M. / Lieber, M.R. / Delarue, M.
External linksJ. Biol. Chem. / PubMed:31138645
MethodsX-ray diffraction
Resolution1.96 - 2.55 Å
Structure data

PDB-6go3:
TdT chimera (Loop1 of pol mu) - apoenzyme
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-6go4:
TdT chimera (Loop1 of pol mu) - binary complex with ddCTP
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

PDB-6go5:
TdT chimera (Loop1 of pol mu) - Ternary complex with 1-nt gapped DNA substrate
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-6go6:
TdT chimera (Loop1 of pol mu) - ternary complex with downstream dsDNA
Method: X-RAY DIFFRACTION / Resolution: 2.09 Å

PDB-6go7:
TdT chimera (Loop1 of pol mu) - full DNA synapsis complex
Method: X-RAY DIFFRACTION / Resolution: 2.55 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-MG:
Unknown entry

ChemComp-DCT:
2',3'-DIDEOXYCYTIDINE 5'-TRIPHOSPHATE

ChemComp-XC5:
2'-deoxy-5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine

Source
  • mus musculus (house mouse)
  • synthetic construct (others)
KeywordsDNA BINDING PROTEIN / NHEJ pathway / DNA bridging / DNA polymerase polX

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