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Open data
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Basic information
Entry | Database: PDB / ID: 4i2f | ||||||
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Title | Binary complex of mouse TdT with ssDNA | ||||||
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![]() | TRANSFERASE/DNA / Terminal transferase / TRANSFERASE-DNA complex | ||||||
Function / homology | ![]() DNA nucleotidylexotransferase / DNA nucleotidylexotransferase activity / DNA modification / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / DNA metabolic process / response to ATP / euchromatin / nuclear matrix / double-strand break repair via nonhomologous end joining / DNA-directed DNA polymerase activity ...DNA nucleotidylexotransferase / DNA nucleotidylexotransferase activity / DNA modification / Hydrolases; Acting on ester bonds; Exodeoxyribonucleases producing 5'-phosphomonoesters / DNA metabolic process / response to ATP / euchromatin / nuclear matrix / double-strand break repair via nonhomologous end joining / DNA-directed DNA polymerase activity / hydrolase activity / chromatin / DNA binding / nucleoplasm / nucleus / metal ion binding / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Gouge, J. / Delarue, M. | ||||||
![]() | ![]() Title: Structures of Intermediates along the Catalytic Cycle of Terminal Deoxynucleotidyltransferase: Dynamical Aspects of the Two-Metal Ion Mechanism. Authors: Gouge, J. / Rosario, S. / Romain, F. / Beguin, P. / Delarue, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 174 KB | Display | ![]() |
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PDB format | ![]() | 133.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 4i27C ![]() 4i28C ![]() 4i29C ![]() 4i2aC ![]() 4i2bC ![]() 4i2cC ![]() 4i2dC ![]() 4i2eC ![]() 4i2gC ![]() 4i2hC ![]() 4i2iC ![]() 4i2jC ![]() 1jmsS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
-Protein / DNA chain , 2 types, 2 molecules AC
#1: Protein | Mass: 45704.008 Da / Num. of mol.: 1 / Fragment: SEE REMARK 999 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: DNA chain | Mass: 1521.077 Da / Num. of mol.: 1 / Source method: obtained synthetically |
-Non-polymers , 4 types, 399 molecules ![](data/chem/img/MG.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/NA.gif)
![](data/chem/img/ZN.gif)
![](data/chem/img/HOH.gif)
#3: Chemical | ChemComp-MG / |
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#4: Chemical | ChemComp-NA / |
#5: Chemical | ChemComp-ZN / |
#6: Water | ChemComp-HOH / |
-Details
Sequence details | PROTEIN FRAGMENT IS THE CATALYTIC CORE OF THE TDT-S (TDT-SMALL) ISOFORM (UNP RESIDUES 132-482, 503-530). |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.41 Å3/Da / Density % sol: 49 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop Details: 20-26% PEG4000, 100 mM HEPES, 200 mM ammonium sulfate, pH 6.0-7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K PH range: 6.0-7.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315r / Detector: CCD / Date: Jul 14, 2012 |
Radiation | Monochromator: single crystal Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.28149 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→47.4 Å / Num. obs: 27387 / % possible obs: 99.8 % / Redundancy: 4.7 % / Biso Wilson estimate: 32.59 Å2 / Rmerge(I) obs: 0.116 |
Reflection shell | Resolution: 2.1→2.21 Å / Redundancy: 4.8 % / Rmerge(I) obs: 0.727 / Mean I/σ(I) obs: 2.1 / Num. unique all: 3936 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1JMS Resolution: 2.1→43.61 Å / Cor.coef. Fo:Fc: 0.942 / Cor.coef. Fo:Fc free: 0.9405 / SU R Cruickshank DPI: 0.179 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 0.203 / SU Rfree Blow DPI: 0.153 / SU Rfree Cruickshank DPI: 0.146 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 38.12 Å2
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Refine analyze | Luzzati coordinate error obs: 0.245 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.1→43.61 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.1→2.18 Å / Total num. of bins used: 14
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Refinement TLS params. | Method: refined / Refine-ID: X-RAY DIFFRACTION
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Refinement TLS group |
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