- PDB-6g7b: Nt2 domain of the TssA component from the type VI secretion syste... -
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ID or keywords:
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Basic information
Entry
Database: PDB / ID: 6g7b
Title
Nt2 domain of the TssA component from the type VI secretion system of Aeromonas hydrophila.
Components
ImpA-related domain protein
Keywords
TRANSPORT PROTEIN / Type VI secretion system component / alpha helical protein / TssA / Aeromonas hydrophila
Function / homology
Type VI secretion system-associated, VCA0119 / Type VI secretion, EvfE, EvfF, ImpA, BimE, VC_A0119, VasJ / ImpA, N-terminal / ImpA, N-terminal, type VI secretion system / ImpA-related domain protein
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 0.9795 Å / Relative weight: 1
Reflection
Resolution: 1.76→101.32 Å / Num. obs: 59629 / % possible obs: 99.9 % / Redundancy: 3.6 % / Biso Wilson estimate: 19.3 Å2 / Rmerge(I) obs: 0.065 / Rpim(I) all: 0.039 / Net I/σ(I): 7
Reflection shell
Resolution: 1.76→1.79 Å
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Processing
Software
Name
Version
Classification
REFMAC
5.8.0189
refinement
xia2
datareduction
pointless
datascaling
PHASER
phasing
Refinement
Method to determine structure: SAD / Resolution: 1.76→101.32 Å / Cor.coef. Fo:Fc: 0.956 / Cor.coef. Fo:Fc free: 0.94 / SU B: 4.904 / SU ML: 0.145 / Cross valid method: THROUGHOUT / ESU R: 0.179 / ESU R Free: 0.162 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.30575
2805
4.7 %
RANDOM
Rwork
0.27227
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obs
0.27382
56717
99.7 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å