+
Open data
-
Basic information
Entry | Database: PDB / ID: 6fms | ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Title | IMISX-EP of Se-LspA | ||||||||||||
![]() |
| ||||||||||||
![]() | HYDROLASE / Serial crystallography / experimental phasing / in meso crystallization / in situ diffraction data collection / membrane protein structure / MEMBRANE PROTEIN | ||||||||||||
Function / homology | ![]() signal peptidase II / signal peptide processing / aspartic-type endopeptidase activity / plasma membrane Similarity search - Function | ||||||||||||
Biological species | ![]() ![]() synthetic construct (others) | ||||||||||||
Method | ![]() ![]() ![]() | ||||||||||||
![]() | Huang, C.-Y. / Olieric, V. / Howe, N. / Warshamanage, R. / Weinert, T. / Panepucci, E. / Vogeley, L. / Basu, S. / Diederichs, K. / Caffrey, M. / Wang, M. | ||||||||||||
Funding support | ![]() ![]()
| ||||||||||||
![]() | ![]() Title: In situ serial crystallography for rapid de novo membrane protein structure determination. Authors: Huang, C.Y. / Olieric, V. / Howe, N. / Warshamanage, R. / Weinert, T. / Panepucci, E. / Vogeley, L. / Basu, S. / Diederichs, K. / Caffrey, M. / Wang, M. | ||||||||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 155 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 121.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.1 MB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 3.2 MB | Display | |
Data in XML | ![]() | 29.7 KB | Display | |
Data in CIF | ![]() | 36.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6fmrC ![]() 6fmtC ![]() 6fmvC ![]() 6fmwC ![]() 6fmxC ![]() 6fmyC C: citing same article ( |
---|---|
Similar structure data |
-
Links
-
Assembly
Deposited unit | ![]()
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| ||||||||
2 | ![]()
| ||||||||
3 | ![]()
| ||||||||
4 | ![]()
| ||||||||
Unit cell |
|
-
Components
#1: Protein | Mass: 21287.877 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: lspA, ls, PA4559 / Production host: ![]() ![]() #2: Protein/peptide | #3: Chemical | ChemComp-OLC / ( #4: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 3.78 Å3/Da / Density % sol: 67.49 % |
---|---|
Crystal grow | Temperature: 293 K / Method: lipidic cubic phase Details: 35 % PEG 400, 100 mM MES, pH 5.6, and 100 mM ammonium phosphate monobasic |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS EIGER X 16M / Detector: PIXEL / Date: Feb 16, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97858 Å / Relative weight: 1 |
Reflection | Resolution: 3→46.27 Å / Num. obs: 41032 / % possible obs: 99.9 % / Redundancy: 22.7 % / CC1/2: 0.99 / Rrim(I) all: 0.31 / Net I/σ(I): 9.14 |
Reflection shell | Resolution: 3→3.08 Å / Redundancy: 22.7 % / Mean I/σ(I) obs: 1.45 / Num. unique obs: 3031 / CC1/2: 0.4 / Rrim(I) all: 3.46 / % possible all: 99.8 |
-
Processing
Software |
| ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: ![]()
| ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3→46.268 Å
|