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Structure paper

TitleIn situ serial crystallography for rapid de novo membrane protein structure determination.
Journal, issue, pagesCommun Biol, Vol. 1, Page 124-124, Year 2018
Publish dateFeb 2, 2018 (structure data deposition date)
AuthorsHuang, C.Y. / Olieric, V. / Howe, N. / Warshamanage, R. / Weinert, T. / Panepucci, E. / Vogeley, L. / Basu, S. / Diederichs, K. / Caffrey, M. / Wang, M.
External linksCommun Biol / PubMed:30272004
MethodsX-ray diffraction
Resolution1.79 - 3 Å
Structure data

PDB-6fmr:
IMISX-EP of Se-PepTSt
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

PDB-6fms:
IMISX-EP of Se-LspA
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-6fmt:
IMISX-EP of Hg-BacA Soaking SAD
Method: X-RAY DIFFRACTION / Resolution: 3 Å

PDB-6fmv:
IMISX-EP of Hg-BacA soaking SIRAS
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6fmw:
IMISX-EP of Hg-BacA cocrystallization
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-6fmx:
IMISX-EP of W-PgpB
Method: X-RAY DIFFRACTION / Resolution: 1.79 Å

PDB-6fmy:
IMISX-EP of S-PepTSt
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-78M:
(2S)-2,3-DIHYDROXYPROPYL(7Z)-PENTADEC-7-ENOATE

ChemComp-PO4:
PHOSPHATE ION

ChemComp-HOH:
WATER

ChemComp-OLC:
(2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate

ChemComp-HG:
Unknown entry

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-FLC:
CITRATE ANION

ChemComp-WO4:
TUNGSTATE(VI)ION

ChemComp-PE5:
3,6,9,12,15,18,21,24-OCTAOXAHEXACOSAN-1-OL / precipitant*YM

Source
  • streptococcus thermophilus (strain atcc baa-250 / lmg 18311) (bacteria)
  • pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (bacteria)
  • synthetic construct (others)
  • escherichia coli k-12 (bacteria)
  • bacillus subtilis (strain 168) (bacteria)
KeywordsTRANSPORT PROTEIN / Serial crystallography / experimental phasing / in meso crystallization / in situ diffraction data collection / membrane protein structure. / HYDROLASE / membrane protein structure / MEMBRANE PROTEIN

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