Protein / Protein/peptide , 2 types, 3 molecules ABC
#1: Protein
NAD-dependentproteindeacylasesirtuin-5, mitochondrial / Regulatory protein SIR2 homolog 5
Mass: 31495.012 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Danio rerio (zebrafish) / Gene: sirt5, si:ch211-121a2.1 / Production host: Escherichia coli (E. coli) References: UniProt: Q6DHI5, Hydrolases; Acting on carbon-nitrogen bonds, other than peptide bonds; In linear amides
#2: Protein/peptide
3(S)-(naphthylthio)succinyl-CPS1peptide
Mass: 1210.398 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) Homo sapiens (human)
Resolution: 2.5→19.85 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.911 / SU B: 24.622 / SU ML: 0.446 / Cross valid method: THROUGHOUT / ESU R: 0.48 / ESU R Free: 0.333 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.31055
1279
5 %
RANDOM
Rwork
0.24158
-
-
-
obs
0.24513
24278
99.56 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK