+Open data
-Basic information
Entry | Database: PDB / ID: 6f4i | ||||||
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Title | Crystal structure of Drosophila melanogaster SNF | ||||||
Components | U1 small nuclear ribonucleoprotein A | ||||||
Keywords | SPLICING / SNF / U2A'. RRM / Evolution | ||||||
Function / homology | Function and homology information primary sex determination, soma / snRNA stem-loop binding / pre-mRNA intronic pyrimidine-rich binding / mRNA Splicing - Major Pathway / female germ-line sex determination / small nuclear ribonucleoprotein complex / poly(U) RNA binding / U2 snRNP / U1 snRNP / oogenesis ...primary sex determination, soma / snRNA stem-loop binding / pre-mRNA intronic pyrimidine-rich binding / mRNA Splicing - Major Pathway / female germ-line sex determination / small nuclear ribonucleoprotein complex / poly(U) RNA binding / U2 snRNP / U1 snRNP / oogenesis / precatalytic spliceosome / regulation of alternative mRNA splicing, via spliceosome / U2 snRNA binding / U1 snRNA binding / catalytic step 2 spliceosome / RNA splicing / spliceosomal complex / mRNA splicing, via spliceosome / ribonucleoprotein complex / mRNA binding / protein-containing complex / identical protein binding / nucleus Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.49 Å | ||||||
Authors | Weber, G. / Holton, N. / Hall, K.B. / DeKoster, G. / Wahl, M.C. | ||||||
Citation | Journal: Nat Commun / Year: 2018 Title: Molecular principles underlying dual RNA specificity in the Drosophila SNF protein. Authors: Weber, G. / DeKoster, G.T. / Holton, N. / Hall, K.B. / Wahl, M.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6f4i.cif.gz | 238.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6f4i.ent.gz | 194.6 KB | Display | PDB format |
PDBx/mmJSON format | 6f4i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6f4i_validation.pdf.gz | 468.5 KB | Display | wwPDB validaton report |
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Full document | 6f4i_full_validation.pdf.gz | 476.2 KB | Display | |
Data in XML | 6f4i_validation.xml.gz | 27.9 KB | Display | |
Data in CIF | 6f4i_validation.cif.gz | 42.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/f4/6f4i ftp://data.pdbj.org/pub/pdb/validation_reports/f4/6f4i | HTTPS FTP |
-Related structure data
Related structure data | 6f4gC 6f4hC 6f4jC 1urnS S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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Unit cell |
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-Components
#1: Protein | Mass: 11082.006 Da / Num. of mol.: 6 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Gene: snf, D25, fs(1)1621, liz, CG4528 / Production host: Escherichia coli (E. coli) / References: UniProt: P43332 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.89 Å3/Da / Density % sol: 57.44 % |
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Crystal grow | Temperature: 277 K / Method: evaporation Details: 0.1 M Tris-HCl, pH 7.5, 0.2 M sodium chloride, 1 M sodium citrate |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: BESSY / Beamline: 14.1 / Wavelength: 0.918417 Å |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Jul 28, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.918417 Å / Relative weight: 1 |
Reflection | Resolution: 1.49→50 Å / Num. obs: 125117 / % possible obs: 98.6 % / Redundancy: 4.5 % / Rrim(I) all: 0.034 / Rsym value: 0.03 / Net I/σ(I): 22.15 |
Reflection shell | Resolution: 1.49→1.58 Å |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1URN Resolution: 1.49→24.329 Å / SU ML: 0.15 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 20.68
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.49→24.329 Å
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Refine LS restraints |
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LS refinement shell |
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