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Open data
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Basic information
Entry | Database: PDB / ID: 6f3z | ||||||
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Title | Complex of E. coli LolA and periplasmic domain of LolC | ||||||
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![]() | PROTEIN TRANSPORT / Lipoprotein trafficking | ||||||
Function / homology | ![]() lipoprotein releasing activity / protein localization to outer membrane / lipoprotein localization to outer membrane / plasma membrane protein complex / lipoprotein transport / ATP-binding cassette (ABC) transporter complex / outer membrane-bounded periplasmic space / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kaplan, E. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Insights into bacterial lipoprotein trafficking from a structure of LolA bound to the LolC periplasmic domain. Authors: Kaplan, E. / Greene, N.P. / Crow, A. / Koronakis, V. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 178.4 KB | Display | ![]() |
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PDB format | ![]() | 140.1 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 460 KB | Display | ![]() |
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Full document | ![]() | 470.4 KB | Display | |
Data in XML | ![]() | 31.6 KB | Display | |
Data in CIF | ![]() | 44.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6f49C ![]() 6fhmC ![]() 1iwlS ![]() 5naaS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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2 | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Refine code: _
NCS ensembles :
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Components
#1: Protein | Mass: 25623.129 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Protein | Mass: 22935.209 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.39 Å3/Da / Density % sol: 48.6 % |
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Crystal grow | Temperature: 288.15 K / Method: vapor diffusion, sitting drop Details: 45% w/v Poly(acrylic acid sodium salt) 2100, 100 mM HEPES pH 6.5 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Apr 8, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9763 Å / Relative weight: 1 |
Reflection | Resolution: 2→73.01 Å / Num. obs: 61745 / % possible obs: 95.8 % / Redundancy: 10.2 % / Rmerge(I) obs: 0.103 / Net I/σ(I): 10.6 |
Reflection shell | Resolution: 2→2.05 Å / Redundancy: 9.9 % / Mean I/σ(I) obs: 2.2 / Num. unique all: 4379 / CC1/2: 0.883 / Rsym value: 0.844 / % possible all: 97 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 5NAA, 1IWL Resolution: 2→73.01 Å / Cor.coef. Fo:Fc: 0.963 / Cor.coef. Fo:Fc free: 0.945 / SU B: 4.715 / SU ML: 0.126 / Cross valid method: THROUGHOUT / ESU R: 0.192 / ESU R Free: 0.174 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 45.619 Å2
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Refinement step | Cycle: 1 / Resolution: 2→73.01 Å
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Refine LS restraints |
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