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- PDB-4v0o: Crystal structure of BBS1N in complex with ARL6DN, soaked with lead -
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Open data
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Basic information
Entry | Database: PDB / ID: 4v0o | ||||||
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Title | Crystal structure of BBS1N in complex with ARL6DN, soaked with lead | ||||||
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![]() | HYDROLASE/STRUCTURAL PROTEIN / HYDROLASE-STRUCTURAL PROTEIN COMPLEX / BBSOME / GTP / COAT COMPLEX | ||||||
Function / homology | ![]() BBSome / non-motile cilium assembly / protein localization to cilium / axoneme / cilium assembly / vesicle-mediated transport / intracellular protein transport / GTPase activity / GTP binding / metal ion binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Mourao, A. / Lorentzen, E. | ||||||
![]() | ![]() Title: Structural Basis for Membrane Targeting of the Bbsome by Arl6 Authors: Mourao, A. / Nager, A.R. / Nachury, M.V. / Lorentzen, E. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 371.3 KB | Display | ![]() |
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PDB format | ![]() | 305.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.4 MB | Display | ![]() |
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Full document | ![]() | 1.4 MB | Display | |
Data in XML | ![]() | 71 KB | Display | |
Data in CIF | ![]() | 95.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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4 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 18806.646 Da / Num. of mol.: 4 / Fragment: GTPASE, RESIDUES 16-180 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Protein | Mass: 46368.062 Da / Num. of mol.: 4 / Fragment: WD40, RESIDUES 1-425 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Chemical | ChemComp-GTP / #4: Chemical | ChemComp-MG / #5: Chemical | ChemComp-PB / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.73 Å3/Da / Density % sol: 67.02 % / Description: NONE |
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Crystal grow | Details: 29% PEG400, 0.1M TRIS PH 8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 2MF / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9497 Å / Relative weight: 1 |
Reflection | Resolution: 3.35→50 Å / Num. obs: 56947 / % possible obs: 99.6 % / Observed criterion σ(I): 1 / Redundancy: 8.2 % / Biso Wilson estimate: 113.5 Å2 / Rmerge(I) obs: 0.11 / Net I/σ(I): 8.6 |
Reflection shell | Resolution: 3.35→3.47 Å / Redundancy: 7.5 % / Rmerge(I) obs: 0.81 / Mean I/σ(I) obs: 1.6 / % possible all: 96.8 |
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Processing
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Refinement | Method to determine structure: OTHER Starting model: NONE Resolution: 3.351→96.273 Å / SU ML: 0.53 / σ(F): 1.91 / Phase error: 28.04 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 3.351→96.273 Å
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Refine LS restraints |
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LS refinement shell |
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