+Open data
-Basic information
Entry | Database: PDB / ID: 4v0l | ||||||
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Title | Crystal structure of the CrARL6DN in the GTP bound form | ||||||
Components | ARF-LIKE SMALL GTPASE | ||||||
Keywords | HYDROLASE | ||||||
Function / homology | Function and homology information protein localization to cilium / axoneme / cilium assembly / vesicle-mediated transport / intracellular protein transport / GTPase activity / GTP binding Similarity search - Function | ||||||
Biological species | CHLAMYDOMONAS REINHARDTII (plant) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.197 Å | ||||||
Authors | Mourao, A. / Lorentzen, E. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2014 Title: Structural Basis for Membrane Targeting of the Bbsome by Arl6 Authors: Mourao, A. / Nager, A.R. / Nachury, M.V. / Lorentzen, E. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 4v0l.cif.gz | 86.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb4v0l.ent.gz | 66.5 KB | Display | PDB format |
PDBx/mmJSON format | 4v0l.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/v0/4v0l ftp://data.pdbj.org/pub/pdb/validation_reports/v0/4v0l | HTTPS FTP |
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-Related structure data
Related structure data | 4v0kC 4v0mC 4v0nC 4v0oC 2h57S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 18806.646 Da / Num. of mol.: 2 / Fragment: GTPASE, RESIDUES 16-180 Source method: isolated from a genetically manipulated source Source: (gene. exp.) CHLAMYDOMONAS REINHARDTII (plant) / Plasmid: PEC-A-HT-HIS / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: A8JF99 #2: Chemical | #3: Chemical | #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 3.97 Å3/Da / Density % sol: 69.04 % / Description: NONE |
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Crystal grow | Details: 30% V/V PENTAERYTHRITOL ETHOXYLATE, 50MM BIS-TRIS PH 6.5, 50MM AMMONIUM SULFATE |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X06DA / Wavelength: 1.0332 |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.0332 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 29942 / % possible obs: 99.9 % / Observed criterion σ(I): 1.5 / Redundancy: 12.7 % / Biso Wilson estimate: 43.52 Å2 / Rmerge(I) obs: 0.03 / Net I/σ(I): 21.1 |
Reflection shell | Resolution: 2.2→2.32 Å / Redundancy: 12.2 % / Rmerge(I) obs: 0.55 / Mean I/σ(I) obs: 1.5 / % possible all: 99.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 2H57 Resolution: 2.197→48.515 Å / SU ML: 0.29 / σ(F): 1.91 / Phase error: 23.12 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.197→48.515 Å
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Refine LS restraints |
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LS refinement shell |
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