Mass: 34430.543 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: WE mutated this residue to alter the substrate specifictiy Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: B4417_3194 / Production host: Escherichia coli (E. coli) / References: UniProt: A0A164TT67, UniProt: Q45462*PLUS
Resolution: 1.42→63.4 Å / Cor.coef. Fo:Fc: 0.961 / Cor.coef. Fo:Fc free: 0.934 / SU B: 1.429 / SU ML: 0.056 / Cross valid method: THROUGHOUT / ESU R: 0.073 / ESU R Free: 0.077 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.21933
2196
4.8 %
RANDOM
Rwork
0.18078
-
-
-
obs
0.1827
43364
98.19 %
-
Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å