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- PDB-6euv: Structure of the midlink and cap-binding domains of influenza A p... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6euv | ||||||
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Title | Structure of the midlink and cap-binding domains of influenza A polymerase PB2 subunit with a bound azaindole cap-binding inhibitor (VX-787) | ||||||
![]() | Polymerase basic protein 2 | ||||||
![]() | RNA BINDING PROTEIN / Influenza A polymerase / PB2 subunit / cap-binding domain / cap-binding inhibitor | ||||||
Function / homology | ![]() cap snatching / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / host cell mitochondrion / 7-methylguanosine mRNA capping / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / virion component / viral RNA genome replication / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / DNA-templated transcription ...cap snatching / symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / host cell mitochondrion / 7-methylguanosine mRNA capping / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / virion component / viral RNA genome replication / RNA-dependent RNA polymerase activity / virus-mediated perturbation of host defense response / DNA-templated transcription / host cell nucleus / RNA binding Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cusack, S. / Lethier, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: Capped RNA primer binding to influenza polymerase and implications for the mechanism of cap-binding inhibitors. Authors: Pflug, A. / Gaudon, S. / Resa-Infante, P. / Lethier, M. / Reich, S. / Schulze, W.M. / Cusack, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 234.6 KB | Display | ![]() |
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PDB format | ![]() | 189.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 3.2 MB | Display | ![]() |
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Full document | ![]() | 3.2 MB | Display | |
Data in XML | ![]() | 42.9 KB | Display | |
Data in CIF | ![]() | 56.4 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6euwC ![]() 6euxSC ![]() 6euyC ![]() 6evjC ![]() 6evkC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS domain:
NCS domain segments: Component-ID: _ / Beg auth comp-ID: ASN / Beg label comp-ID: ASN / Refine code: _
NCS ensembles :
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Components
#1: Protein | Mass: 32524.322 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Details: cap-midlink double domain residues 247-536 Source: (gene. exp.) ![]() Gene: PB2 / Plasmid: pETM11 / Production host: ![]() ![]() #2: Chemical | ChemComp-21G / ( #3: Chemical | ChemComp-NI / #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.05 Å3/Da / Density % sol: 59.67 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: Crystals were obtained by mixing protein at 16 mg per mL mixed with 2 mM VX-787 in 0.1 M MES pH6, 0.7 M sodium formate pH 6. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M-F / Detector: PIXEL / Date: Oct 3, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.966 Å / Relative weight: 1 |
Reflection | Resolution: 2.7→50 Å / Num. obs: 38468 / % possible obs: 94.8 % / Redundancy: 1.69 % / CC1/2: 0.991 / Rmerge(I) obs: 0.091 / Net I/σ(I): 5.99 |
Reflection shell | Resolution: 2.7→2.77 Å / Redundancy: 1.55 % / Rmerge(I) obs: 0.698 / Mean I/σ(I) obs: 1.02 / CC1/2: 0.508 / % possible all: 94.2 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 6EUX Resolution: 2.7→43.35 Å / Cor.coef. Fo:Fc: 0.911 / Cor.coef. Fo:Fc free: 0.877 / SU B: 16.915 / SU ML: 0.336 / Cross valid method: THROUGHOUT / ESU R: 3.957 / ESU R Free: 0.392 / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 49.627 Å2
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Refinement step | Cycle: 1 / Resolution: 2.7→43.35 Å
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Refine LS restraints |
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