+Open data
-Basic information
Entry | Database: PDB / ID: 6eto | ||||||
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Title | Atomic resolution structure of RNase A (data collection 5) | ||||||
Components | Ribonuclease pancreatic | ||||||
Keywords | HYDROLASE / Raman microspectroscopy / ribonuclease / atomic resolution / radiation damage / photodamage | ||||||
Function / homology | Function and homology information pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.02 Å | ||||||
Authors | Caterino, M. / Vergara, A. / Merlino, A. | ||||||
Citation | Journal: Chembiochem / Year: 2018 Title: The Alkylquinolone Repertoire of Pseudomonas aeruginosa is Linked to Structural Flexibility of the FabH-like 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) Biosynthesis Enzyme PqsBC. Authors: Witzgall, F. / Depke, T. / Hoffmann, M. / Empting, M. / Bronstrup, M. / Muller, R. / Blankenfeldt, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6eto.cif.gz | 99.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6eto.ent.gz | 79 KB | Display | PDB format |
PDBx/mmJSON format | 6eto.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/et/6eto ftp://data.pdbj.org/pub/pdb/validation_reports/et/6eto | HTTPS FTP |
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-Related structure data
Related structure data | 6eszC 6et0C 6et1C 6et2C 6et3C 1kf3S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 13708.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Bos taurus (cattle) / References: UniProt: P61823, EC: 3.1.27.5 | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.68 % |
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Crystal grow | Temperature: 293 K / Method: liquid diffusion Details: Free-interface diffusion of a 10 uL of 30 mg ml-1 protein solution, 20 mM sodium citrate at pH 5.3 agaist 10 ul isopropanol 99.9% isopropanol. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SLS / Beamline: X10SA / Wavelength: 0.7749 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Sep 26, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.7749 Å / Relative weight: 1 |
Reflection | Resolution: 1.02→50 Å / Num. obs: 54830 / % possible obs: 98.9 % / Redundancy: 6.6 % / Rmerge(I) obs: 0.094 / Net I/σ(I): 10.15 |
Reflection shell | Resolution: 1.02→1.06 Å / Redundancy: 6.4 % / Rmerge(I) obs: 0.451 / Mean I/σ(I) obs: 4.29 / % possible all: 97.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1KF3 Resolution: 1.02→50 Å / Cross valid method: FREE R-VALUE
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Displacement parameters | Biso mean: 19.17 Å2 | ||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.02→50 Å
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