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TitleThe Alkylquinolone Repertoire of Pseudomonas aeruginosa is Linked to Structural Flexibility of the FabH-like 2-Heptyl-3-hydroxy-4(1H)-quinolone (PQS) Biosynthesis Enzyme PqsBC.
Journal, issue, pagesChembiochem, Vol. 19, Page 1531-1544, Year 2018
Publish dateOct 25, 2017 (structure data deposition date)
AuthorsWitzgall, F. / Depke, T. / Hoffmann, M. / Empting, M. / Bronstrup, M. / Muller, R. / Blankenfeldt, W.
External linksChembiochem / PubMed:29722462
MethodsX-ray diffraction
Resolution1.02 - 2.65 Å
Structure data

PDB-6esz:
Crystal structure of PqsBC from Pseudomonas aeruginosa (crystal form 1)
Method: X-RAY DIFFRACTION / Resolution: 1.84 Å

PDB-6et0:
Crystal structure of PqsBC (C129A) mutant from Pseudomonas aeruginosa (crystal form 1)
Method: X-RAY DIFFRACTION / Resolution: 1.53 Å

PDB-6et1:
Crystal structure of PqsBC from Pseudomonas aeruginosa (crystal form 2)
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-6et2:
Crystal structure of PqsBC (C129A) mutant from Pseudomonas aeruginosa (crystal form 3)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-6et3:
Crystal structure of PqsBC (C129S) mutant from Pseudomonas aeruginosa (crystal form 4)
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-6eto:
Atomic resolution structure of RNase A (data collection 5)
Method: X-RAY DIFFRACTION / Resolution: 1.02 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-MRD:
(4R)-2-METHYLPENTANE-2,4-DIOL / precipitant*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-MPO:
3[N-MORPHOLINO]PROPANE SULFONIC ACID / pH buffer*YM

ChemComp-BU3:
(R,R)-2,3-BUTANEDIOL

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM

Source
  • pseudomonas aeruginosa pao1 (bacteria)
  • bos taurus (domestic cattle)
KeywordsTRANSFERASE / Pseudomonas Quinolone Signal / FabH / HHQ / HYDROLASE / Raman microspectroscopy / ribonuclease / atomic resolution / radiation damage / photodamage

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