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Open data
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Basic information
Entry | Database: PDB / ID: 6etl | ||||||
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Title | Atomic resolution structure of RNase A (data collection 2) | ||||||
![]() | Ribonuclease pancreatic | ||||||
![]() | HYDROLASE / Raman microspectroscopy / ribonuclease / atomic resolution / radiation damage / photodamage | ||||||
Function / homology | ![]() pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Caterino, M. / Vergara, A. / Merlino, A. | ||||||
![]() | ![]() Title: Raman-markers of X-ray radiation damage of proteins. Authors: Vergara, A. / Caterino, M. / Merlino, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 92.4 KB | Display | ![]() |
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PDB format | ![]() | 76.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Arichive directory | ![]() ![]() | HTTPS FTP |
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-Related structure data
Related structure data | ![]() 6etkC ![]() 6etmC ![]() 6etnC ![]() 6etpC ![]() 6etqC ![]() 6etrC C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Components
#1: Protein | Mass: 13708.326 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.04 Å3/Da / Density % sol: 39.68 % |
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Crystal grow | Temperature: 293 K / Method: liquid diffusion / pH: 5.3 Details: Free-interface diffusion of a 10 uL of 30 mg ml-1 protein solution, 20 mM sodium citrate at pH 5.3 agaist 10 ul isopropanol 99.9% isopropanol. |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Sep 26, 2011 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.7749 Å / Relative weight: 1 |
Reflection | Resolution: 0.85→50 Å / Num. obs: 95598 / % possible obs: 97.8 % / Redundancy: 3.3 % / Rmerge(I) obs: 0.047 / Net I/σ(I): 38.7 |
Reflection shell | Resolution: 0.85→0.88 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.353 / Mean I/σ(I) obs: 2.5 / % possible all: 95.9 |
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Processing
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Refinement | Resolution: 0.85→50 Å / Cross valid method: FREE R-VALUE
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Refinement step | Cycle: LAST / Resolution: 0.85→50 Å
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