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- PDB-6em4: State B architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing th... -

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Entry
Database: PDB / ID: 6em4
TitleState B architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes
Components
  • (60S ribosomal protein ...) x 19
  • (Ribosome biogenesis protein ...) x 6
  • 25S ribosomal RNA
  • 5.8S ribosomal RNA
  • ATP-dependent RNA helicase HAS1
  • Eukaryotic translation initiation factor 6
  • Nucleolar protein 16
  • Pescadillo homolog
  • Proteasome-interacting protein CIC1
  • Protein MAK16
  • Ribosomal RNA-processing protein 1
  • Ribosome production factor 1
  • internal transcribed spacer 1
  • rRNA-processing protein EBP2
KeywordsRIBOSOME / Large Subunit Biogenesis Nucleolus
Function/homologyEukaryotic rRNA processing / Mak16 protein / cell cycle DNA replication / snoRNA release from pre-rRNA / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein BRX1 / WDR74/Nsa1 / Nucleolar, Nop52 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 ...Eukaryotic rRNA processing / Mak16 protein / cell cycle DNA replication / snoRNA release from pre-rRNA / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein BRX1 / WDR74/Nsa1 / Nucleolar, Nop52 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 / Domain of unknown function DUF4217 / Eukaryotic rRNA processing protein EBP2 / nuclear division / Pescadillo / Ribosome biogenesis protein 15, RNA recognition motif / Mak16 protein C-terminal region / exonucleolytic trimming to generate mature 5'-end of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosome biogenesis protein Nop16 / regulation of rRNA processing / BOP1NT (NUC169) domain / Domain of unknown function (DUF4217) / Nucleolar protein,Nop52 / PeBoW complex / Pescadillo N-terminus / rRNA primary transcript binding / Brix domain / Brix domain profile. / Translation initiation factor IF6 / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / actomyosin contractile ring assembly / eIF-6 family / Brix domain / mature ribosome assembly / regulation of cell division / RNA-dependent ATPase activity / ribosomal subunit export from nucleus / Ribosomal L28e/Mak16 / BRCT domain, a BRCA1 C-terminus domain / cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / Ribosomal L28e protein family / ATPase activator activity / ribosomal large subunit binding / preribosome, small subunit precursor / DEAD-box subfamily ATP-dependent helicases signature. / preribosome, large subunit precursor / ATP-dependent RNA helicase DEAD-box, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L13e, conserved site / TRASH domain / Ribosomal protein L13e / 60S ribosomal protein L18a/ L20, eukaryotes / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L6e signature. / RNA helicase, DEAD-box type, Q motif / DEAD-box RNA helicase Q motif profile. / proteasome binding / SRP-dependent cotranslational protein targeting to membrane / GTP hydrolysis and joining of the 60S ribosomal subunit / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / RNA secondary structure unwinding / Ribosomal protein L36e signature. / Formation of a pool of free 40S subunits / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / 60S ribosomal protein L6E / Ribosomal protein L7, eukaryotic / Ribosomal protein L30, N-terminal / Ribosomal protein L7A/L8 / Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) / Ribosomal protein L36e / Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) / 60S ribosomal protein L4, C-terminal domain / Ribosomal protein L35A / Ribosomal protein L14e domain / Ribosomal protein L18e signature. / Ribosomal protein L18e, conserved site / ribosomal large subunit export from nucleus / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L18e / Ribosomal protein L13e / ribosomal large subunit biogenesis / L13a-mediated translational silencing of Ceruloplasmin expression / Ribosomal protein L15e, conserved site / Ribosomal protein L15e signature. / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / ribosomal small subunit biogenesis / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L37e, conserved site / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L7, eukaryotic/archaeal / Ribosomal protein L1e signature. / Ribosomal protein L37e signature. / Ribosomal protein L30, central domain, archaeal type / Ribosomal protein L37e / Ribosomal protein L24e-related / Ribosomal protein L22/L17, eukaryotic/archaeal / ATP-dependent RNA helicase activity
Function and homology information
Specimen sourceSaccharomyces cerevisiae / / yeast /
MethodElectron microscopy (4.1 Å resolution / Particle / Single particle) / Transmission electron microscopy
AuthorsKater, L. / Cheng, J. / Barrio-Garcia, C. / Hurt, E. / Beckmann, R.
CitationJournal: Cell / Year: 2017
Title: Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.
Authors: Lukas Kater / Matthias Thoms / Clara Barrio-Garcia / Jingdong Cheng / Sherif Ismail / Yasar Luqman Ahmed / Gert Bange / Dieter Kressler / Otto Berninghausen / Irmgard Sinning / Ed Hurt / Roland Beckmann
Abstract: Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural ...Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural information, this process is poorly understood. Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 Å to 4.5 Å resolution, thereby providing insights into their sequential folding and assembly pathway. Besides revealing distinct immature rRNA conformations, we map 25 assembly factors in six different assembly states. Notably, the Nsa1-Rrp1-Rpf1-Mak16 module stabilizes the solvent side of the 60S subunit, and the Erb1-Ytm1-Nop7 complex organizes and connects through Erb1's meandering N-terminal extension, eight assembly factors, three ribosomal proteins, and three 25S rRNA domains. Our structural snapshots reveal the order of integration and compaction of the six major 60S domains within early nucleolar 60S particles developing stepwise from the solvent side around the exit tunnel to the central protuberance.
Copyright: 2017 The Authors. Published by Elsevier Inc. All rights reserved.
Validation Report
SummaryFull reportAbout validation report
DateDeposition: Oct 1, 2017 / Release: Dec 27, 2017
RevisionDateData content typeGroupCategoryItemProviderType
1.0Dec 27, 2017Structure modelrepositoryInitial release
1.1Mar 21, 2018Structure modelOther / Structure summaryentity / pdbx_database_status_entity.pdbx_description / _pdbx_database_status.process_site

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Structure visualization

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Assembly

Deposited unit
x: Ribosome production factor 1
F: 60S ribosomal protein L7-A
3: Protein MAK16
4: Ribosomal RNA-processing protein 1
5: Ribosome biogenesis protein NSA1
A: Ribosome biogenesis protein BRX1
J: rRNA-processing protein EBP2
u: Ribosome biogenesis protein RLP24
v: Nucleolar protein 16
y: Eukaryotic translation initiation factor 6
B: 60S ribosomal protein L3
C: 60S ribosomal protein L4-A
e: 60S ribosomal protein L32
E: 60S ribosomal protein L6-A
f: 60S ribosomal protein L33-A
G: 60S ribosomal protein L8-A
h: 60S ribosomal protein L35-A
i: 60S ribosomal protein L36-B
j: 60S ribosomal protein L37-A
L: 60S ribosomal protein L13-A
M: 60S ribosomal protein L14-A
N: 60S ribosomal protein L15-A
O: 60S ribosomal protein L16-A
P: 60S ribosomal protein L17-A
Q: 60S ribosomal protein L18-A
S: 60S ribosomal protein L20-A
V: 60S ribosomal protein L23-A
Y: 60S ribosomal protein L26-A
1: 25S ribosomal RNA
2: 5.8S ribosomal RNA
D: ATP-dependent RNA helicase HAS1
K: Proteasome-interacting protein CIC1
n: Pescadillo homolog
o: Ribosome biogenesis protein 15
t: Ribosome biogenesis protein RLP7
6: internal transcribed spacer 1
H: Ribosome biogenesis protein ERB1


Theoretical massNumber of molelcules
Total (without water)2,258,68237
Polyers2,258,68237
Non-polymers00
Water0
1


TypeNameSymmetry operationNumber
identity operation1_5551

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Components

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Protein/peptide , 9 types, 9 molecules x34JvyDKn

#1: Protein/peptide Ribosome production factor 1 / Ribosome biogenesis protein RPF1


Mass: 35184.387 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P38805
#3: Protein/peptide Protein MAK16 / Maintenance of killer protein 16


Mass: 35754.605 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P10962
#4: Protein/peptide Ribosomal RNA-processing protein 1


Mass: 33250.531 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P35178
#7: Protein/peptide rRNA-processing protein EBP2 / EBNA1-binding protein homolog


Mass: 49842.613 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P36049
#9: Protein/peptide Nucleolar protein 16


Mass: 26954.447 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P40007
#10: Protein/peptide Eukaryotic translation initiation factor 6 / eIF-6


Mass: 26476.605 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q12522
#31: Protein/peptide ATP-dependent RNA helicase HAS1 / Helicase associated with SET1 protein 1


Mass: 56798.348 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q03532, EC:3.6.4.13 (RNA helicase)
#32: Protein/peptide Proteasome-interacting protein CIC1 / Core interacting component 1


Mass: 42596.691 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P38779
#33: Protein/peptide Pescadillo homolog / Nucleolar protein 7 / Ribosomal RNA-processing protein 13


Mass: 69984.148 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P53261

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60S ribosomal protein ... , 19 types, 19 molecules FBCeEfGhijLMNOPQSVY

#2: Protein/peptide 60S ribosomal protein L7-A / / L6 / Large ribosomal subunit protein uL30-A / RP11 / YL8


Mass: 27686.281 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P05737
#11: Protein/peptide 60S ribosomal protein L3 / / Large ribosomal subunit protein uL3 / Maintenance of killer protein 8 / RP1 / Trichodermin resistance protein / YL1


Mass: 43850.793 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P14126
#12: Protein/peptide 60S ribosomal protein L4-A / / L2 / Large ribosomal subunit protein uL4-A / RP2 / YL2


Mass: 39159.125 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P10664
#13: Protein/peptide 60S ribosomal protein L32 / / Large ribosomal subunit protein eL32


Mass: 14809.441 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P38061
#14: Protein/peptide 60S ribosomal protein L6-A / / L17 / Large ribosomal subunit protein eL6-A / RP18 / YL16


Mass: 20000.564 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q02326
#15: Protein/peptide 60S ribosomal protein L33-A / / L37 / Large ribosomal subunit protein eL33-A / RP47 / YL37


Mass: 12177.130 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P05744
#16: Protein/peptide 60S ribosomal protein L8-A / / L4 / L4-2 / L7a-1 / Large ribosomal subunit protein eL8-A / Maintenance of killer protein 7 / RP6 / YL5


Mass: 28175.820 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P17076
#17: Protein/peptide 60S ribosomal protein L35-A / / Large ribosomal subunit protein uL29-A


Mass: 13942.640 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P0CX84
#18: Protein/peptide 60S ribosomal protein L36-B / / L39 / Large ribosomal subunit protein eL36-B / YL39


Mass: 11162.285 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:O14455
#19: Protein/peptide 60S ribosomal protein L37-A / / L43 / Large ribosomal subunit protein eL37-A / YL35 / YP55


Mass: 9877.395 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P49166
#20: Protein/peptide 60S ribosomal protein L13-A / / Large ribosomal subunit protein eL13-A


Mass: 22604.164 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q12690
#21: Protein/peptide 60S ribosomal protein L14-A / / Large ribosomal subunit protein eL14-A


Mass: 15195.066 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P36105
#22: Protein/peptide 60S ribosomal protein L15-A / / L13 / Large ribosomal subunit protein eL15-A / RP15R / YL10 / YP18


Mass: 24482.357 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P05748
#23: Protein/peptide 60S ribosomal protein L16-A / / L13a / L21 / Large ribosomal subunit protein uL13-A / RP22 / YL15


Mass: 22247.227 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P26784
#24: Protein/peptide 60S ribosomal protein L17-A / / L20A / Large ribosomal subunit protein uL22-A / YL17


Mass: 20589.518 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P05740
#25: Protein/peptide 60S ribosomal protein L18-A / / Large ribosomal subunit protein eL18-A / RP28


Mass: 20609.252 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P0CX49
#26: Protein/peptide 60S ribosomal protein L20-A / / L18a / Large ribosomal subunit protein eL20-A


Mass: 20478.852 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P0CX23
#27: Protein/peptide 60S ribosomal protein L23-A / / L17a / Large ribosomal subunit protein uL14-A / YL32


Mass: 14493.950 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P0CX41
#28: Protein/peptide 60S ribosomal protein L26-A / / L33 / Large ribosomal subunit protein uL24-A / YL33


Mass: 14265.784 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P05743

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Ribosome biogenesis protein ... , 6 types, 6 molecules 5AuotH

#5: Protein/peptide Ribosome biogenesis protein NSA1 / / NOP7-associated protein 1


Mass: 51982.457 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P53136
#6: Protein/peptide Ribosome biogenesis protein BRX1 /


Mass: 33585.414 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q08235
#8: Protein/peptide Ribosome biogenesis protein RLP24 / / Ribosomal protein L24-like


Mass: 24027.650 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q07915
#34: Protein/peptide Ribosome biogenesis protein 15 / / Nucleolar protein 15


Mass: 25499.186 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P53927
#35: Protein/peptide Ribosome biogenesis protein RLP7 / / Ribosomal protein L7-like


Mass: 36621.074 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:P40693
#37: Protein/peptide Ribosome biogenesis protein ERB1 / / Eukaryotic ribosome biogenesis protein 1


Mass: 91830.609 Da / Num. of mol.: 1 / Details: Backbone model
Source: (natural) Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Strain: ATCC 204508 / S288c / References: UniProt:Q04660

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RNA chain , 3 types, 3 molecules 126

#29: RNA chain 25S ribosomal RNA /


Mass: 1097493.875 Da / Num. of mol.: 1 / Details: Backbone model / Source: (natural) Saccharomyces cerevisiae / References: GenBank:834774822
#30: RNA chain 5.8S ribosomal RNA


Mass: 50682.922 Da / Num. of mol.: 1 / Details: Backbone model / Source: (natural) Saccharomyces cerevisiae / References: GenBank:1214879358
#36: RNA chain internal transcribed spacer 1


Mass: 74308.391 Da / Num. of mol.: 1 / Details: Backbone model / Source: (natural) Saccharomyces cerevisiae / References: GenBank:1102641490

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Experimental details

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Experiment

ExperimentMethod: ELECTRON MICROSCOPY
EM experimentAggregation state: PARTICLE / Reconstruction method: SINGLE PARTICLE

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Sample preparation

ComponentName: Nsa1-TAP Flag-Ytm1 / Type: RIBOSOME
Details: Nucleolar 60S biogenesis intermediates purified via Nsa1-TAP Flag-Ytm1
Entity ID: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37
Source: NATURAL
Source (natural)Organism: Saccharomyces cerevisiae
Buffer solutionpH: 7.5
SpecimenEmbedding applied: NO / Shadowing applied: NO / Staining applied: NO / Vitrification applied: YES
VitrificationCryogen name: ETHANE

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Electron microscopy imaging

Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company
MicroscopyMicroscope model: FEI TITAN KRIOS
Electron gunElectron source: FIELD EMISSION GUN / Accelerating voltage: 300 kV / Illumination mode: FLOOD BEAM
Electron lensMode: BRIGHT FIELD / Cs: 2.7 mm
Specimen holderCryogen: NITROGEN / Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER
Image recordingElectron dose: 27 e/Å2 / Detector mode: INTEGRATING / Film or detector model: FEI FALCON II (4k x 4k)
Image scansSampling size: 14 microns / Dimension width: 4096 / Dimension height: 4096 / Movie frames/image: 10 / Used frames/image: 1-6

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Processing

EM software
IDNameVersionCategory
1GautomatchPARTICLE SELECTION
4GctfCTF CORRECTION
9RELION2.0INITIAL EULER ASSIGNMENT
10RELION2.0FINAL EULER ASSIGNMENT
11RELION2.0CLASSIFICATION
12RELION2.0RECONSTRUCTION
CTF correctionType: NONE
SymmetryPoint symmetry: C1
3D reconstructionResolution: 4.1 Å / Resolution method: FSC 0.143 CUT-OFF / Number of particles: 65539 / Symmetry type: POINT
Atomic model buildingDetails: Backbone model / Ref protocol: RIGID BODY FIT / Ref space: REAL

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