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- PDB-6eic: Crystal structure of Rv0183, a Monoglyceride Lipase from Mycobact... -
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Open data
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Basic information
Entry | Database: PDB / ID: 6eic | ||||||
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Title | Crystal structure of Rv0183, a Monoglyceride Lipase from Mycobacterium Tuberculosis | ||||||
![]() | Mycobacterium Tuberculosis Monoglyceride Lipase | ||||||
![]() | HYDROLASE / Monoglycerid Lipase / Lipase / Alpha/Beta hydrolase | ||||||
Function / homology | ![]() symbiont-mediated activation of host apoptosis / acylglycerol lipase / glycerolipid catabolic process / monoacylglycerol lipase activity / lipase activity / peptidoglycan-based cell wall / extracellular region / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Aschauer, P. / Pavkov-Keller, T. / Oberer, M. | ||||||
Funding support | ![]()
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![]() | ![]() Title: The crystal structure of monoacylglycerol lipase from M. tuberculosis reveals the basis for specific inhibition. Authors: Aschauer, P. / Zimmermann, R. / Breinbauer, R. / Pavkov-Keller, T. / Oberer, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 174.2 KB | Display | ![]() |
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PDB format | ![]() | 139.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 290.4 KB | Display | ![]() |
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Full document | ![]() | 298.7 KB | Display | |
Data in XML | ![]() | 17.7 KB | Display | |
Data in CIF | ![]() | 28.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components on special symmetry positions |
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Components
#1: Protein | Mass: 30298.533 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: Rv0183 / Production host: ![]() ![]() #2: Chemical | ChemComp-SO4 / | #3: Chemical | #4: Chemical | ChemComp-NO3 / | #5: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.32 % |
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Crystal grow | Temperature: 293.15 K / Method: vapor diffusion, sitting drop / pH: 7.8 Details: 0.03M sodium nitrate, 0.03M di-sodium hydrogenphosphate, 0.03M ammonium sulfate, 0.1M MOPS/HEPES, 12% 2-Methyl 2,4 pentandiol, 12% PEG1000, 12% PEG3350. Microseeding |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: DECTRIS PILATUS 6M / Detector: PIXEL / Date: Mar 1, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97895 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→49.209 Å / Num. obs: 77304 / % possible obs: 100 % / Redundancy: 9.6 % / CC1/2: 0.998 / Rmerge(I) obs: 0.105 / Rpim(I) all: 0.035 / Net I/σ(I): 16.4 |
Reflection shell | Resolution: 1.8→1.84 Å / Redundancy: 8.7 % / Rmerge(I) obs: 0.853 / Mean I/σ(I) obs: 2.6 / Num. unique obs: 4549 / CC1/2: 0.734 / Rpim(I) all: 0.307 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→49.209 Å
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Refine LS restraints |
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LS refinement shell |
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