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Open data
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Basic information
Entry | Database: PDB / ID: 6e6y | ||||||
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Title | Dieckmann cyclase, NcmC | ||||||
![]() | Dieckmann cyclase, NcmC | ||||||
![]() | BIOSYNTHETIC PROTEIN / Dieckmann cyclase / off-loading / Dieckmann condensation | ||||||
Function / homology | Alpha/Beta hydrolase fold / NcmC![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Cogan, D.P. / Nair, S.K. | ||||||
![]() | ![]() Title: Structural Basis for Enzymatic Off-Loading of Hybrid Polyketides by Dieckmann Condensation. Authors: Cogan, D.P. / Ly, J. / Nair, S.K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 121.6 KB | Display | ![]() |
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PDB format | ![]() | 92.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 449.3 KB | Display | ![]() |
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Full document | ![]() | 454.9 KB | Display | |
Data in XML | ![]() | 26.1 KB | Display | |
Data in CIF | ![]() | 38.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28814.383 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Chemical | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.19 Å3/Da / Density % sol: 43.76 % |
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Crystal grow | Temperature: 282 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 1.75 M ammonium sulfate, 1.9% (w/v) PEG 400, 0.1 M HEPES pH 7.0, 3 mM DTT |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 300 mm CCD / Detector: CCD / Date: Aug 22, 2015 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97858 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→80.43 Å / Num. obs: 64619 / % possible obs: 99 % / Redundancy: 13.3 % / Rmerge(I) obs: 0.087 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 1.6→1.657 Å / Rmerge(I) obs: 0.69 / Num. unique obs: 5773 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.263 Å2
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Refinement step | Cycle: 1 / Resolution: 1.6→80.43 Å
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Refine LS restraints |
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