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TitleStructural Basis for Enzymatic Off-Loading of Hybrid Polyketides by Dieckmann Condensation.
Journal, issue, pagesAcs Chem. Biol., Year 2020
Publish dateJul 25, 2018 (structure data deposition date)
AuthorsCogan, D.P. / Ly, J. / Nair, S.K.
External linksAcs Chem. Biol. / PubMed:33017142
MethodsX-ray diffraction
Resolution1.55 - 1.6 Å
Structure data

PDB-6e6t:
Dieckmann cyclase, NcmC, bound to cerulenin
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6e6u:
Variant C89S of Dieckmann cyclase, NcmC
Method: X-RAY DIFFRACTION / Resolution: 1.55 Å

PDB-6e6y:
Dieckmann cyclase, NcmC
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

Chemicals

ChemComp-SO4:
SULFATE ION

ChemComp-HVV:
(4S,5R)-4,5-dihydroxy-5-[(3E,6E)-octa-3,6-dien-1-yl]pyrrolidin-2-one

ChemComp-HOH:
WATER

Source
  • saccharothrix syringae (bacteria)
KeywordsLYASE/ANTIBIOTIC / Dieckmann cyclase / off-loading / Dieckmann condensation / LYASE-ANTIBIOTIC complex / BIOSYNTHETIC PROTEIN

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