Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Homologous Recombination (HRR) / post-anal tail morphogenesis / DNA repair complex / inner cell mass cell proliferation / mesoderm development / embryonic organ development / somitogenesis / animal organ morphogenesis ...Homologous DNA Pairing and Strand Exchange / Resolution of D-loop Structures through Holliday Junction Intermediates / HDR through Homologous Recombination (HRR) / post-anal tail morphogenesis / DNA repair complex / inner cell mass cell proliferation / mesoderm development / embryonic organ development / somitogenesis / animal organ morphogenesis / double-strand break repair via homologous recombination / multicellular organism growth / in utero embryonic development / apoptotic process / negative regulation of apoptotic process / protein-containing complex / DNA binding / nucleoplasm Similarity search - Function
Partner and localiser of BRCA2, WD40 domain / Partner and localizer of BRCA2 / Partner and localizer of BRCA2 WD40 domain / WD40-repeat-containing domain superfamily / WD40/YVTN repeat-like-containing domain superfamily Similarity search - Domain/homology
Evidence: gel filtration, X-filtered Noisy experiment shows the two chains of the homodimer to be oriented anti-parallel to each other. N15 T1/T2 measurements demonstrate it's a dimer.
Type
Name
Symmetry operation
Number
identity operation
1_555
1
Buried area
2000 Å2
ΔGint
-12 kcal/mol
Surface area
11730 Å2
NMR ensembles
Data
Criteria
Number of conformers (submitted / calculated)
20 / 100
target function
Representative
Model #1
lowest energy
-
Components
#1: Protein
PartnerandlocalizerofBRCA2
Mass: 7948.166 Da / Num. of mol.: 2 / Fragment: residues 1-60 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Mus musculus (house mouse) / Gene: Palb2 / Production host: Escherichia coli (E. coli) / References: UniProt: Q3U0P1
-
Experimental details
-
Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D TROSY-HSQC
1
2
1
isotropic
1
2D 1H-13C HSQC aliphatic
1
3
1
isotropic
1
3D TROSY-HN(CO)CA
1
4
1
isotropic
1
3D TROSY-HNCA
1
5
1
isotropic
1
3D TROSY-HN(CO)CACB
1
7
1
isotropic
1
3D TROSY-HN(CA)CB
1
15
1
isotropic
1
3D TROSY-HNCO
1
6
1
isotropic
1
3D TROSY-HBHA(CO)NH
1
8
1
isotropic
1
3D (H)CCH-TOCSY
1
10
1
isotropic
1
3D CCH-TOCSY
1
9
1
isotropic
1
AROMATIC CCH-TOCSY
1
11
1
isotropic
1
3D 1H-13C NOESY
1
13
1
isotropic
1
3D 1H-13C NOESY aromatic
1
12
1
isotropic
1
3D simultaneous 13C-aromatic,13C-aliphatic,15N edited 1H-1H NOESY
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