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- PDB-6dks: Structure of the Rbpj-SHARP-DNA Repressor Complex -

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Basic information

Entry
Database: PDB / ID: 6dks
TitleStructure of the Rbpj-SHARP-DNA Repressor Complex
Components
  • DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')
  • DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')
  • Maltose/maltodextrin-binding periplasmic protein
  • Recombining binding protein suppressor of hairless
KeywordsTRANSCRIPTION/DNA / Notch Signaling / Rbpjk / SHARP / MINT / CSL / Transport-DNA Binding-DNA complex / TRANSCRIPTION-DNA complex
Function / homology
Function and homology information


regulation of timing of cell differentiation / club cell differentiation / blood vessel endothelial cell fate specification / positive regulation of ERBB signaling pathway / blood vessel lumenization / arterial endothelial cell fate commitment / regulation of generation of precursor metabolites and energy / sebaceous gland development / epidermal cell fate specification / determination of heart left/right asymmetry ...regulation of timing of cell differentiation / club cell differentiation / blood vessel endothelial cell fate specification / positive regulation of ERBB signaling pathway / blood vessel lumenization / arterial endothelial cell fate commitment / regulation of generation of precursor metabolites and energy / sebaceous gland development / epidermal cell fate specification / determination of heart left/right asymmetry / positive regulation of ephrin receptor signaling pathway / auditory receptor cell fate commitment / dorsal aorta morphogenesis / pulmonary valve development / endocardium morphogenesis / MAML1-RBP-Jkappa- ICN1 complex / NOTCH1 Intracellular Domain Regulates Transcription / RUNX3 regulates NOTCH signaling / positive regulation of transcription of Notch receptor target / secondary heart field specification / hair follicle maturation / CSL-Notch-Mastermind transcription factor complex / Notch-HLH transcription pathway / aortic valve development / epithelial to mesenchymal transition involved in endocardial cushion formation / pituitary gland development / heart induction / positive regulation of cell proliferation involved in heart morphogenesis / cardiac left ventricle morphogenesis / endocardium development / labyrinthine layer blood vessel development / myeloid dendritic cell differentiation / cardiac muscle cell myoblast differentiation / negative regulation of smooth muscle cell migration / regulation of cell adhesion involved in heart morphogenesis / atrioventricular canal development / somitogenesis / inflammatory response to antigenic stimulus / ventricular trabecula myocardium morphogenesis / negative regulation of ossification / positive regulation of BMP signaling pathway / somatic stem cell population maintenance / artery morphogenesis / negative regulation of cold-induced thermogenesis / heart looping / ventricular septum morphogenesis / blood vessel remodeling / humoral immune response / hemopoiesis / cell fate commitment / positive regulation of cardiac muscle cell proliferation / outflow tract morphogenesis / negative regulation of smooth muscle cell apoptotic process / keratinocyte differentiation / negative regulation of cell differentiation / Notch signaling pathway / epithelial to mesenchymal transition / carbohydrate transmembrane transporter activity / transcription repressor complex / maltose binding / maltose transport / maltodextrin transmembrane transport / B cell differentiation / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / positive regulation of smooth muscle cell proliferation / negative regulation of neuron projection development / protein-DNA complex / heart development / angiogenesis / positive regulation of canonical Wnt signaling pathway / regulation of gene expression / neuron differentiation / regulation of cell population proliferation / outer membrane-bounded periplasmic space / transcription regulator complex / DNA-binding transcription activator activity, RNA polymerase II-specific / DNA-binding transcription factor binding / sequence-specific DNA binding / RNA polymerase II-specific DNA-binding transcription factor binding / DNA-binding transcription factor activity, RNA polymerase II-specific / defense response to bacterium / periplasmic space / RNA polymerase II cis-regulatory region sequence-specific DNA binding / negative regulation of cell population proliferation / negative regulation of DNA-templated transcription / chromatin binding / positive regulation of gene expression / positive regulation of cell population proliferation / regulation of transcription by RNA polymerase II / nucleolus / positive regulation of DNA-templated transcription / protein-containing complex binding / negative regulation of transcription by RNA polymerase II / positive regulation of transcription by RNA polymerase II / protein-containing complex / DNA binding / nucleoplasm / nucleus / cytoplasm
Similarity search - Function
LAG1, DNA binding domain / Beta-trefoil DNA-binding domain / RBP-J/Cbf11/Cbf12, DNA binding / Beta-trefoil domain superfamily / RBP-J/Cbf11, DNA binding domain superfamily / RBP-Jkappa, IPT domain / Suppressor of hairless-like / Beta-trefoil DNA-binding domain / LAG1, DNA binding / TIG domain ...LAG1, DNA binding domain / Beta-trefoil DNA-binding domain / RBP-J/Cbf11/Cbf12, DNA binding / Beta-trefoil domain superfamily / RBP-J/Cbf11, DNA binding domain superfamily / RBP-Jkappa, IPT domain / Suppressor of hairless-like / Beta-trefoil DNA-binding domain / LAG1, DNA binding / TIG domain / LAG1, DNA binding / Beta-trefoil DNA-binding domain / Trefoil (Acidic Fibroblast Growth Factor, subunit A) - #50 / Trefoil (Acidic Fibroblast Growth Factor, subunit A) / Trefoil / p53-like transcription factor, DNA-binding / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Periplasmic binding protein-like II / D-Maltodextrin-Binding Protein; domain 2 / Immunoglobulin E-set / Immunoglobulin-like fold / Immunoglobulins / Immunoglobulin-like / Sandwich / 3-Layer(aba) Sandwich / Mainly Beta / Alpha Beta
Similarity search - Domain/homology
alpha-maltose / DNA / DNA (> 10) / Maltose/maltodextrin-binding periplasmic protein / Recombining binding protein suppressor of hairless
Similarity search - Component
Biological speciesMus musculus (house mouse)
Escherichia coli O157:H7 (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.78 Å
AuthorsKovall, R.A. / VanderWielen, B.D. / Yuan, Z.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Cancer Institute (NIH/NCI)NCI CA178974 United States
CitationJournal: Cell Rep / Year: 2019
Title: Structural and Functional Studies of the RBPJ-SHARP Complex Reveal a Conserved Corepressor Binding Site.
Authors: Yuan, Z. / VanderWielen, B.D. / Giaimo, B.D. / Pan, L. / Collins, C.E. / Turkiewicz, A. / Hein, K. / Oswald, F. / Borggrefe, T. / Kovall, R.A.
History
DepositionMay 30, 2018Deposition site: RCSB / Processing site: RCSB
Revision 1.0Jan 2, 2019Provider: repository / Type: Initial release
Revision 1.1Feb 20, 2019Group: Author supporting evidence / Data collection / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.2Jul 17, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.journal_volume / _citation.page_first / _citation.page_last / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.3Dec 4, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 2.0Jul 29, 2020Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Non-polymer description / Structure summary
Category: atom_site / chem_comp ...atom_site / chem_comp / entity / entity_name_com / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_molecule_features / pdbx_nonpoly_scheme / struct_conn / struct_conn_type / struct_site / struct_site_gen
Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _entity.formula_weight / _entity.pdbx_description / _entity.type
Description: Carbohydrate remediation / Provider: repository / Type: Remediation
Revision 2.1Oct 11, 2023Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary
Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model
Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')
B: DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')
C: Recombining binding protein suppressor of hairless
D: Maltose/maltodextrin-binding periplasmic protein
E: DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')
F: DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')
G: Recombining binding protein suppressor of hairless
H: Maltose/maltodextrin-binding periplasmic protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)203,47210
Polymers202,7878
Non-polymers6852
Water00
1


  • Idetical with deposited unit
  • defined by author
  • Evidence: isothermal titration calorimetry
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area14570 Å2
ΔGint-79 kcal/mol
Surface area78350 Å2
2
A: DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')
B: DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')
C: Recombining binding protein suppressor of hairless
D: Maltose/maltodextrin-binding periplasmic protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)101,7365
Polymers101,3944
Non-polymers3421
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6870 Å2
ΔGint-31 kcal/mol
Surface area39870 Å2
MethodPISA
3
E: DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')
F: DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')
G: Recombining binding protein suppressor of hairless
H: Maltose/maltodextrin-binding periplasmic protein
hetero molecules


Theoretical massNumber of molelcules
Total (without water)101,7365
Polymers101,3944
Non-polymers3421
Water0
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area6690 Å2
ΔGint-37 kcal/mol
Surface area39500 Å2
MethodPISA
Unit cell
Length a, b, c (Å)54.450, 231.570, 90.260
Angle α, β, γ (deg.)90.00, 99.88, 90.00
Int Tables number4
Space group name H-MP1211

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Components

#1: DNA chain DNA (5'-D(*AP*AP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*T)-3')


Mass: 4503.949 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Mus musculus (house mouse)
#2: DNA chain DNA (5'-D(*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*A)-3')


Mass: 4673.059 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) Mus musculus (house mouse)
#3: Protein Recombining binding protein suppressor of hairless / J kappa-recombination signal-binding protein / RBP-J kappa


Mass: 47869.641 Da / Num. of mol.: 2 / Fragment: residues 53-474
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Mus musculus (house mouse) / Gene: Rbpj, Igkjrb1, Igkrsbp, Rbpsuh / Production host: Escherichia coli (E. coli) / References: UniProt: P31266
#4: Protein Maltose/maltodextrin-binding periplasmic protein / MMBP / Maltodextrin-binding protein / Maltose-binding protein / MBP


Mass: 44347.074 Da / Num. of mol.: 2 / Fragment: residues 28-392
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Escherichia coli O157:H7 (bacteria) / Gene: malE, Z5632, ECs5017 / Production host: Escherichia coli (E. coli) / References: UniProt: P0AEY0
#5: Polysaccharide alpha-D-glucopyranose-(1-4)-alpha-D-glucopyranose / alpha-maltose


Type: oligosaccharide, Oligosaccharide / Class: Nutrient / Mass: 342.297 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Details: oligosaccharide / References: alpha-maltose
DescriptorTypeProgram
DGlcpa1-4DGlcpa1-ROHGlycam Condensed SequenceGMML 1.0
WURCS=2.0/1,2,1/[a2122h-1a_1-5]/1-1/a4-b1WURCSPDB2Glycan 1.1.0
[][a-D-Glcp]{[(4+1)][a-D-Glcp]{}}LINUCSPDB-CARE

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.78 Å3/Da / Density % sol: 55.81 %
Crystal growTemperature: 277 K / Method: microbatch / pH: 6.6
Details: Bis-Tris pH6.6 100mM NaCl 40% PEG400 200mM NDSB-256

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Data collection

DiffractionMean temperature: 200 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 21-ID-F / Wavelength: 0.978 Å
DetectorType: MARMOSAIC 225 mm CCD / Detector: CCD / Date: Feb 8, 2012
RadiationMonochromator: Diamond [111] / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.978 Å / Relative weight: 1
ReflectionResolution: 2.78→50 Å / Num. obs: 54503 / % possible obs: 99.3 % / Redundancy: 3.7 % / Biso Wilson estimate: 72.68 Å2 / Rmerge(I) obs: 0.084 / Net I/σ(I): 14.9
Reflection shellResolution: 2.8→2.85 Å / Rmerge(I) obs: 0.48 / Num. unique obs: 3903 / % possible all: 95.14

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Processing

Software
NameVersionClassification
BUSTER2.10.2refinement
XDSdata reduction
XDSdata scaling
PHENIXphasing
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: 3BRG, 3H4Z

3brg

3h4z


Resolution: 2.78→38.53 Å / Cor.coef. Fo:Fc: 0.9193 / Cor.coef. Fo:Fc free: 0.8941 / SU R Cruickshank DPI: 2.291 / Cross valid method: THROUGHOUT / σ(F): 0 / SU R Blow DPI: 1.014 / SU Rfree Blow DPI: 0.301 / SU Rfree Cruickshank DPI: 0.314
RfactorNum. reflection% reflectionSelection details
Rfree0.2279 2756 5.06 %RANDOM
Rwork0.1949 ---
obs0.1966 54503 98.85 %-
Displacement parametersBiso mean: 77.8 Å2
Baniso -1Baniso -2Baniso -3
1--19.7861 Å20 Å213.699 Å2
2--6.2534 Å20 Å2
3---13.5327 Å2
Refine analyzeLuzzati coordinate error obs: 0.369 Å
Refinement stepCycle: 1 / Resolution: 2.78→38.53 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms12174 1218 46 0 13438
Refine LS restraints
Refine-IDTypeDev idealNumberRestraint functionWeight
X-RAY DIFFRACTIONt_bond_d0.00813877HARMONIC2
X-RAY DIFFRACTIONt_angle_deg0.9619190HARMONIC2
X-RAY DIFFRACTIONt_dihedral_angle_d4292SINUSOIDAL2
X-RAY DIFFRACTIONt_incorr_chiral_ct
X-RAY DIFFRACTIONt_pseud_angle
X-RAY DIFFRACTIONt_trig_c_planes268HARMONIC2
X-RAY DIFFRACTIONt_gen_planes1930HARMONIC5
X-RAY DIFFRACTIONt_it13877HARMONIC20
X-RAY DIFFRACTIONt_nbd
X-RAY DIFFRACTIONt_omega_torsion2.18
X-RAY DIFFRACTIONt_other_torsion18.89
X-RAY DIFFRACTIONt_improper_torsion
X-RAY DIFFRACTIONt_chiral_improper_torsion1887SEMIHARMONIC5
X-RAY DIFFRACTIONt_sum_occupancies
X-RAY DIFFRACTIONt_utility_distance
X-RAY DIFFRACTIONt_utility_angle
X-RAY DIFFRACTIONt_utility_torsion
X-RAY DIFFRACTIONt_ideal_dist_contact14466SEMIHARMONIC4
LS refinement shellResolution: 2.78→2.85 Å / Total num. of bins used: 20
RfactorNum. reflection% reflection
Rfree0.2723 207 5.3 %
Rwork0.2489 3696 -
all0.2501 3903 -
obs--95.14 %
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
12.69213.2902-1.2411.4695-1.66191.28520.0204-0.15310.2113-0.12350.0021-0.3065-0.18310.2225-0.02250.4327-0.3127-0.0196-0.0931-0.1758-0.0966-28.435444.272-52.8423
2-1.0603-5.09923.30786.9650.40333.50910.03110.1510.3218-0.12510.05520.1457-0.1825-0.2316-0.08630.65560.2318-0.1913-0.6720.107-0.0838-41.09640.6758-67.5445
36.3488-0.2669-1.93210.9836-2.6640.9940.05580.3816-0.1987-0.1707-0.05190.025-0.0302-0.0528-0.00390.33640.0631-0.1656-0.32210.167-0.0385-39.33833.0369-84.7012
44.4113-3.8148-2.12220.10841.55753.89070.0537-0.15940.23420.1259-0.02940.2639-0.2666-0.0108-0.02430.5920.2789-0.3140.17340.4951-0.2346-47.932736.1065-83.7127
52.1485-5.2916-1.51721.0014-4.85060.18130.0604-0.06360.2097-0.03880.0331-0.1766-0.62250.038-0.09360.2322-0.1248-0.1141-0.63260.1507-0.4003-35.681535.3005-71.1078
60.5532-1.2723-1.79727.07243.33352.40010.0086-0.2720.2292-0.05390.13940.0578-0.2628-0.2065-0.1480.7826-0.2079-0.0786-0.2757-0.0535-0.1495-36.262745.9838-57.1072
71.0523-0.20620.53313.80590.1171.7822-0.0125-0.04770.1815-0.3333-0.00270.2705-0.0927-0.15760.0151-0.17620.01470.07280.06090.0285-0.0164-38.32491.9824-61.1441
82.2406-0.5109-0.22162.5494-0.85133.31380.03670.11690.28730.0364-0.07690.5031-0.44320.02930.0402-0.13030.04190.0409-0.02030.0742-0.0507-39.20614.9977-60.5389
94.53132.0586-1.34082.322-0.25678.5943-0.33050.248-0.1731-0.2403-0.1601-0.5667-1.20871.68690.4906-0.1956-0.27070.01450.13030.2083-0.1554-18.698622.8798-70.767
103.983-1.40014.11782.3129-1.33296.0415-0.413-0.18080.37380.68660.0574-0.2595-0.61-0.13130.3556-0.0148-0.0388-0.0323-0.16870.0324-0.1954-27.3731-7.0787-42.2841
113.22121.4417-1.05732.8885-1.69753.90820.4956-0.05970.72870.1988-0.0810.3674-0.52080.1535-0.41460.2687-0.11110.0721-0.3262-0.0341-0.13125.723727.5224-33.851
121.70210.9243-1.10853.7523-2.15543.43310.3914-0.42680.1480.4514-0.3071-0.0853-0.20930.5512-0.08420.2104-0.1513-0.0927-0.1826-0.0353-0.29356.407612.0636-20.1413
131.56181.64342.37127.2931.28992.1980.05940.0166-0.0184-0.08980.0160.7651-0.2289-0.5476-0.07540.0798-0.1075-0.1713-0.08340.0043-0.0397-13.02774.8221-22.6374
144.59024.1865-5.07622.89915.01592.52140.02660.45140.0248-0.0070.1468-0.1781-0.35760.2303-0.17340.2347-0.102-0.2564-0.22990.056-0.247-13.6536-5.9928-35.6193
157.54331.5686-3.59915.80932.1819-1.3821-0.0174-0.06680.01990.17570.15-0.29560.05180.143-0.1327-0.38130.02690.2030.43360.22210.0181-9.288312.6907-69.7728
165.01040.2455-2.2751.0780.65131.72980.0440.10950.0637-0.2064-0.051-0.3448-0.0090.08420.0070.3778-0.2284-0.32740.2364-0.5026-0.042513.2025-79.60153.0776
1710.4585-4.4063-0.80516.85814.5311-0.0236-0.0409-0.1111-0.180.16210.15370.06630.13510.203-0.11280.0126-0.2568-0.1439-0.6066-0.0167-0.1111.3388-72.873218.276
186.29831.6655-3.70622.0614-1.79742.69040.1054-0.3906-0.20790.0895-0.032-0.2468-0.1540.3177-0.07340.15630.1003-0.5004-0.40880.10670.42726.3562-67.937233.6671
192.44475.29331.36751.3034-3.52753.4702-0.03350.0902-0.17730.14260.00320.230.2198-0.10280.03030.46710.3087-0.33740.18240.5117-0.046423.0333-69.32842.288
200.0657-1.94594.04644.3311-6.03292.38840.04160.171-0.28-0.22470.1351-0.33870.1150.0015-0.1768-0.2171-0.1076-0.2106-0.64780.0050.083620.7631-65.812325.223
216.2541-0.546-2.003312.5414-2.0731-0.2523-0.031-0.0817-0.5494-0.08520.08130.03060.2778-0.245-0.05030.4365-0.0896-0.2597-0.5448-0.20050.291613.1133-78.078915.5043
220.59520.23370.30082.94610.2931.0744-0.0404-0.0148-0.15450.3717-0.0129-0.20770.1309-0.14630.0533-0.1238-0.1080.028-0.08190.0484-0.08435.2477-35.490223.4271
238.475-8.5292-4.24571.57163.048213.5627-0.1086-0.1063-0.01470.2628-0.04610.04540.037-0.01460.15470.3286-0.0905-0.28330.17150.1798-0.17188.5753-44.213430.0894
244.67180.7075-1.22922.83420.3642.4815-0.2750.3759-0.9945-0.1010.319-0.93290.21190.1835-0.044-0.1869-0.12890.0226-0.2645-0.12070.195422.7908-51.984913.192
251.0378-0.58890.75571.8005-0.84095.30070.04020.1361-0.0367-0.4698-0.08960.37890.1071-0.08490.0494-0.1044-0.0769-0.0521-0.04360.0363-0.1502-4.9267-26.78964.3425
263.2552-0.7487-0.10383.8952-0.0732.7627-0.1894-0.31-0.9567-0.2447-0.1731-0.34811.26870.54710.36240.20630.21230.2606-0.21280.2311-0.050515.6523-57.469-26.8212
271.8907-0.5616-0.45152.29240.10333.2093-0.0830.1426-0.0583-0.442-0.2172-0.28480.36280.09820.30010.01840.03450.1244-0.15490.0821-0.13447.0634-40.7183-34.967
280.06181.248-0.24787.46331.13540.8093-0.1390.06880.02360.1694-0.00640.09690.0203-0.42490.1455-0.1706-0.05230.04360.0525-0.0319-0.1736-7.8799-36.7282-20.7027
293.38850.5699-0.90793.44666.38835.14460.13330.1456-0.4383-0.2978-0.24950.0111-0.20940.19690.1162-0.0424-0.01340.0068-0.06610.0692-0.1922-1.3065-25.4613-9.6499
302.49470.1335-4.54213.63852.17574.598-0.09120.32010.067-0.1510.11770.08570.057-0.1801-0.02660.0546-0.3437-0.1127-0.22240.08760.247931.8827-40.8765.4787
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection details
1X-RAY DIFFRACTION1{A|1 - 6}
2X-RAY DIFFRACTION2{A|7 - 11}
3X-RAY DIFFRACTION3{A|12 - 15}
4X-RAY DIFFRACTION4{B|1 - 5}
5X-RAY DIFFRACTION5{B|6 - 10}
6X-RAY DIFFRACTION6{B|11 - 15}
7X-RAY DIFFRACTION7{C|53 - 138}
8X-RAY DIFFRACTION8{C|139 - 205}
9X-RAY DIFFRACTION9{C|206 - 364}
10X-RAY DIFFRACTION10{C|365 - 474}
11X-RAY DIFFRACTION11{D|2 - 111}
12X-RAY DIFFRACTION12{D|112 - 343}
13X-RAY DIFFRACTION13{D|344 - 370}
14X-RAY DIFFRACTION14{D|2777 - 2798}
15X-RAY DIFFRACTION15{D|2799 - 2814}
16X-RAY DIFFRACTION16{E|1 - 4}
17X-RAY DIFFRACTION17{E|5 - 9}
18X-RAY DIFFRACTION18{E|10 - 15}
19X-RAY DIFFRACTION19{F|1 - 5}
20X-RAY DIFFRACTION20{F|6 - 9}
21X-RAY DIFFRACTION21{F|10 - 15}
22X-RAY DIFFRACTION22{G|53 - 131}
23X-RAY DIFFRACTION23{G|132 - 149}
24X-RAY DIFFRACTION24{G|150 - 364}
25X-RAY DIFFRACTION25{G|365 - 474}
26X-RAY DIFFRACTION26{H|3 - 91}
27X-RAY DIFFRACTION27{H|92 - 343}
28X-RAY DIFFRACTION28{H|344 - 370}
29X-RAY DIFFRACTION29{H|2777 - 2798}
30X-RAY DIFFRACTION30{H|2799 - 2813}

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