Entry | Database: PDB / ID: 6dd5 |
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Title | Crystal Structure of the Cas6 Domain of Marinomonas mediterranea MMB-1 Cas6-RT-Cas1 Fusion Protein |
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Components | MMB-1 Cas6 Fused to Maltose Binding Protein,CRISPR-associated endonuclease Cas1 |
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Keywords | HYDROLASE / Crispr Cas Protein / Endoribonuclease |
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Function / homology | Function and homology information
maintenance of CRISPR repeat elements / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / RNA-directed DNA polymerase activity / outer membrane-bounded periplasmic space / endonuclease activity / defense response to virus ...maintenance of CRISPR repeat elements / carbohydrate transmembrane transporter activity / maltose binding / maltose transport / maltodextrin transmembrane transport / ATP-binding cassette (ABC) transporter complex, substrate-binding subunit-containing / RNA-directed DNA polymerase activity / outer membrane-bounded periplasmic space / endonuclease activity / defense response to virus / Hydrolases; Acting on ester bonds / DNA binding / RNA binding / metal ion bindingSimilarity search - Function RNA-directed DNA polymerase (reverse transcriptase), msDNA / CRISPR-associated endonuclease Cas1, N-terminal domain / CRISPR-associated protein Cas1 / CRISPR-associated endonuclease Cas1, C-terminal domain / CRISPR associated protein Cas1 / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein ...RNA-directed DNA polymerase (reverse transcriptase), msDNA / CRISPR-associated endonuclease Cas1, N-terminal domain / CRISPR-associated protein Cas1 / CRISPR-associated endonuclease Cas1, C-terminal domain / CRISPR associated protein Cas1 / Maltose/Cyclodextrin ABC transporter, substrate-binding protein / Solute-binding family 1, conserved site / Bacterial extracellular solute-binding proteins, family 1 signature. / Bacterial extracellular solute-binding protein / Bacterial extracellular solute-binding protein / Reverse transcriptase (RNA-dependent DNA polymerase) / Reverse transcriptase domain / Reverse transcriptase (RT) catalytic domain profile. / Reverse transcriptase/Diguanylate cyclase domain / DNA/RNA polymerase superfamilySimilarity search - Domain/homology |
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Biological species | ![](img/tx_bacteria.gif) Escherichia coli O157:H7 (bacteria)
Marinomonas mediterranea MMB-1 (bacteria) |
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Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.85 Å |
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Authors | Stamos, J.L. / Mohr, G. / Silas, S. / Makarova, K.S. / Markham, L.M. / Yao, J. / Lucas-Elio, P. / Sanchez-Amat, A. / Fire, A.Z. / Koonin, E.V. / Lambowitz, A.M. |
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Citation | Journal: Mol. Cell / Year: 2018 Title: A Reverse Transcriptase-Cas1 Fusion Protein Contains a Cas6 Domain Required for Both CRISPR RNA Biogenesis and RNA Spacer Acquisition. Authors: Mohr, G. / Silas, S. / Stamos, J.L. / Makarova, K.S. / Markham, L.M. / Yao, J. / Lucas-Elio, P. / Sanchez-Amat, A. / Fire, A.Z. / Koonin, E.V. / Lambowitz, A.M. |
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History | Deposition | May 9, 2018 | Deposition site: RCSB / Processing site: RCSB |
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Revision 1.0 | Oct 17, 2018 | Provider: repository / Type: Initial release |
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Revision 1.1 | Nov 7, 2018 | Group: Data collection / Database references / Category: citation Item: _citation.journal_abbrev / _citation.journal_id_ISSN ..._citation.journal_abbrev / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title |
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Revision 1.2 | Nov 28, 2018 | Group: Data collection / Database references / Category: citation / Item: _citation.journal_volume / _citation.page_first |
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Revision 2.0 | Jul 29, 2020 | Group: Atomic model / Data collection ...Atomic model / Data collection / Derived calculations / Non-polymer description / Structure summary Category: atom_site / chem_comp ...atom_site / chem_comp / entity / entity_name_com / pdbx_branch_scheme / pdbx_chem_comp_identifier / pdbx_entity_branch / pdbx_entity_branch_descriptor / pdbx_entity_branch_link / pdbx_entity_branch_list / pdbx_entity_nonpoly / pdbx_molecule_features / pdbx_nonpoly_scheme / pdbx_struct_assembly_gen / struct_asym / struct_conn / struct_site / struct_site_gen Item: _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ..._atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_asym_id / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.type_symbol / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.pdbx_synonyms / _chem_comp.type / _entity.formula_weight / _entity.pdbx_description / _entity.type / _pdbx_struct_assembly_gen.asym_id_list / _struct_asym.entity_id Description: Carbohydrate remediation / Provider: repository / Type: Remediation |
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Revision 2.1 | Oct 11, 2023 | Group: Data collection / Database references ...Data collection / Database references / Refinement description / Structure summary Category: chem_comp / chem_comp_atom ...chem_comp / chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / struct_ncs_dom_lim Item: _chem_comp.pdbx_synonyms / _database_2.pdbx_DOI ..._chem_comp.pdbx_synonyms / _database_2.pdbx_DOI / _database_2.pdbx_database_accession / _struct_ncs_dom_lim.beg_auth_comp_id / _struct_ncs_dom_lim.beg_label_asym_id / _struct_ncs_dom_lim.beg_label_comp_id / _struct_ncs_dom_lim.beg_label_seq_id / _struct_ncs_dom_lim.end_auth_comp_id / _struct_ncs_dom_lim.end_label_asym_id / _struct_ncs_dom_lim.end_label_comp_id / _struct_ncs_dom_lim.end_label_seq_id |
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