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Open data
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Basic information
Entry | Database: PDB / ID: 6d8q | ||||||
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Title | NMR solution structure of tamapin, mutant DP30/Y31+N | ||||||
![]() | Potassium channel toxin alpha-KTx 5.4 | ||||||
![]() | TOXIN / Tamapin mutant / DP30/Y31+N / CSalpha/beta / SK channels | ||||||
Function / homology | Scorpion short toxins signature. / Scorpion short chain toxin, potassium channel inhibitor / Scorpion short toxin, BmKK2 / Knottin, scorpion toxin-like superfamily / ion channel inhibitor activity / potassium channel regulator activity / toxin activity / extracellular region / Potassium channel toxin alpha-KTx 5.4![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
![]() | del Rio Portilla, F. / Melchor Meneses, C.M. / Titaux Delgado, G.A. / Mayorga Flores, M. | ||||||
![]() | ![]() Title: Novel Blocker of Onco SK3 Channels Derived from Scorpion Toxin Tamapin and Active against Migration of Cancer Cells Authors: Flores, M. / Chantome, A. / Melchor-Meneses, C.M. / Domingo, I. / Titaux Delgado, G.A. / Galindo-Murillo, R. / Vandier, C. / del Rio Portilla, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 183.9 KB | Display | ![]() |
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PDB format | ![]() | 156.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 373.2 KB | Display | ![]() |
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Full document | ![]() | 463.7 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 18.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6d3tC ![]() 6d8hC ![]() 6d8rC ![]() 6d8sC ![]() 6d8tC ![]() 6d8uC ![]() 6d8yC ![]() 6d93C ![]() 6d9oC ![]() 6d9pC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3487.174 Da / Num. of mol.: 1 / Mutation: P30Y, Y31N Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution Contents: 2.0 mM Potassium channel toxin alpha-KTx 5.4, 95% H2O/5% D2O Label: 1H_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 2.0 mM / Component: Potassium channel toxin alpha-KTx 5.4 / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength: 0 Not defined / Label: 1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE III / Manufacturer: Bruker / Model: AVANCE III / Field strength: 700 MHz |
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Processing
NMR software |
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Refinement | Method: molecular dynamics / Software ordinal: 1 / Details: used also for simulated annealing | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |