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Open data
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Basic information
Entry | Database: PDB / ID: 6d3t | ||||||
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Title | NMR solution structure of tamapin, mutant DP30 | ||||||
![]() | Potassium channel toxin alpha-KTx 5.4 | ||||||
![]() | TOXIN / Tamapin mutant / DP30 / CS-alpha/beta / SK channels | ||||||
Function / homology | Scorpion short toxins signature. / Scorpion short chain toxin, potassium channel inhibitor / Scorpion short toxin, BmKK2 / Knottin, scorpion toxin-like superfamily / ion channel inhibitor activity / potassium channel regulator activity / toxin activity / extracellular region / Potassium channel toxin alpha-KTx 5.4![]() | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / molecular dynamics | ||||||
![]() | del Rio Portilla, F. / Melchor Meneses, C.M. / Titaux Delgado, G. / Mayorga Flores, M. | ||||||
![]() | ![]() Title: Novel Blocker of Onco SK3 Channels Derived from Scorpion Toxin Tamapin and Active against Migration of Cancer Cells Authors: Mayorga-Flores, M. / Chantome, A. / Melchor Meneses, C.M. / Domingo, I. / Titaux Delgado, G. / Galindo Murillo, R. / Vandier, C. / del Rio Portilla, F. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 178.4 KB | Display | ![]() |
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PDB format | ![]() | 152.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 377 KB | Display | ![]() |
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Full document | ![]() | 468.7 KB | Display | |
Data in XML | ![]() | 10.9 KB | Display | |
Data in CIF | ![]() | 18 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 6d8hC ![]() 6d8qC ![]() 6d8rC ![]() 6d8sC ![]() 6d8tC ![]() 6d8uC ![]() 6d8yC ![]() 6d93C ![]() 6d9oC ![]() 6d9pC C: citing same article ( |
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Similar structure data | |
Other databases |
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein/peptide | Mass: 3373.071 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Details: mutant DP30 Source: (gene. exp.) ![]() Production host: ![]() ![]() |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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Sample preparation
Details | Type: solution / Contents: 1.0 mM Tamapin mutant DP30, 95% H2O/5% D2O / Label: H1_sample / Solvent system: 95% H2O/5% D2O |
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Sample | Conc.: 1.0 mM / Component: Tamapin mutant DP30 / Isotopic labeling: natural abundance |
Sample conditions | Ionic strength: 0 Not defined / Label: 1 / pH: 6.5 / Pressure: 1 atm / Temperature: 298 K |
-NMR measurement
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 500 MHz |
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Processing
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Refinement | Method: molecular dynamics / Software ordinal: 1 / Details: used also for simulated annealing | ||||||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 200 / Conformers submitted total number: 20 |