+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6cxi | ||||||
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タイトル | Cardiac thin filament decorated with C0C1 fragment of cardiac myosin binding protein C mode 1 | ||||||
要素 |
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キーワード | MOTOR PROTEIN / myosin binding protein C | ||||||
機能・相同性 | 機能・相同性情報 basal body patch / C zone / regulation of muscle filament sliding / striated muscle myosin thick filament / tight junction assembly / regulation of transepithelial transport / morphogenesis of a polarized epithelium / cardiac myofibril / regulation of striated muscle contraction / structural constituent of postsynaptic actin cytoskeleton ...basal body patch / C zone / regulation of muscle filament sliding / striated muscle myosin thick filament / tight junction assembly / regulation of transepithelial transport / morphogenesis of a polarized epithelium / cardiac myofibril / regulation of striated muscle contraction / structural constituent of postsynaptic actin cytoskeleton / profilin binding / protein localization to bicellular tight junction / dense body / Formation of annular gap junctions / Gap junction degradation / Cell-extracellular matrix interactions / Adherens junctions interactions / regulation of stress fiber assembly / positive regulation of ATP-dependent activity / Striated Muscle Contraction / Sensory processing of sound by outer hair cells of the cochlea / Interaction between L1 and Ankyrins / Sensory processing of sound by inner hair cells of the cochlea / regulation of synaptic vesicle endocytosis / A band / ventricular cardiac muscle tissue morphogenesis / structural constituent of muscle / apical junction complex / regulation of focal adhesion assembly / positive regulation of wound healing / myosin binding / maintenance of blood-brain barrier / myofibril / sarcomere organization / NuA4 histone acetyltransferase complex / myosin heavy chain binding / Recycling pathway of L1 / filamentous actin / calyx of Held / ATPase activator activity / EPH-ephrin mediated repulsion of cells / RHO GTPases Activate WASPs and WAVEs / RHO GTPases activate IQGAPs / RHOBTB2 GTPase cycle / heart morphogenesis / cardiac muscle contraction / titin binding / EPHB-mediated forward signaling / phagocytic vesicle / sarcomere / axonogenesis / Translocation of SLC2A4 (GLUT4) to the plasma membrane / RHO GTPases Activate Formins / cell motility / actin filament / FCGR3A-mediated phagocytosis / 加水分解酵素; 酸無水物に作用; 酸無水物に作用・細胞または細胞小器官の運動に関与 / Signaling by high-kinase activity BRAF mutants / Schaffer collateral - CA1 synapse / MAP2K and MAPK activation / structural constituent of cytoskeleton / Regulation of actin dynamics for phagocytic cup formation / platelet aggregation / VEGFA-VEGFR2 Pathway / cellular response to type II interferon / Signaling by RAF1 mutants / Signaling by moderate kinase activity BRAF mutants / Paradoxical activation of RAF signaling by kinase inactive BRAF / Signaling downstream of RAS mutants / Signaling by BRAF and RAF1 fusions / cell-cell junction / Clathrin-mediated endocytosis / actin binding / angiogenesis / cytoskeleton / blood microparticle / hydrolase activity / cell adhesion / positive regulation of cell migration / axon / focal adhesion / ubiquitin protein ligase binding / synapse / positive regulation of gene expression / protein kinase binding / extracellular space / extracellular exosome / ATP binding / identical protein binding / membrane / nucleus / metal ion binding / plasma membrane / cytoplasm / cytosol 類似検索 - 分子機能 | ||||||
生物種 | Homo sapiens (ヒト) | ||||||
手法 | 電子顕微鏡法 / らせん対称体再構成法 / クライオ電子顕微鏡法 / 解像度: 11 Å | ||||||
データ登録者 | Galkin, V.E. / Schroeder, G.F. | ||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: Structure / 年: 2018 タイトル: N-Terminal Domains of Cardiac Myosin Binding Protein C Cooperatively Activate the Thin Filament. 著者: Cristina Risi / Betty Belknap / Eva Forgacs-Lonart / Samantha P Harris / Gunnar F Schröder / Howard D White / Vitold E Galkin / 要旨: Muscle contraction relies on interaction between myosin-based thick filaments and actin-based thin filaments. Myosin binding protein C (MyBP-C) is a key regulator of actomyosin interactions. Recent ...Muscle contraction relies on interaction between myosin-based thick filaments and actin-based thin filaments. Myosin binding protein C (MyBP-C) is a key regulator of actomyosin interactions. Recent studies established that the N'-terminal domains (NTDs) of MyBP-C can either activate or inhibit thin filaments, but the mechanism of their collective action is poorly understood. Cardiac MyBP-C (cMyBP-C) harbors an extra NTD, which is absent in skeletal isoforms of MyBP-C, and its role in regulation of cardiac contraction is unknown. Here we show that the first two domains of human cMyPB-C (i.e., C0 and C1) cooperate to activate the thin filament. We demonstrate that C1 interacts with tropomyosin via a positively charged loop and that this interaction, stabilized by the C0 domain, is required for thin filament activation by cMyBP-C. Our data reveal a mechanism by which cMyBP-C can modulate cardiac contraction and demonstrate a function of the C0 domain. | ||||||
履歴 |
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-構造の表示
ムービー |
ムービービューア |
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構造ビューア | 分子: MolmilJmol/JSmol |
-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6cxi.cif.gz | 533.7 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6cxi.ent.gz | 428.4 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6cxi.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6cxi_validation.pdf.gz | 1011.9 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6cxi_full_validation.pdf.gz | 1.1 MB | 表示 | |
XML形式データ | 6cxi_validation.xml.gz | 82.5 KB | 表示 | |
CIF形式データ | 6cxi_validation.cif.gz | 131.9 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/cx/6cxi ftp://data.pdbj.org/pub/pdb/validation_reports/cx/6cxi | HTTPS FTP |
-関連構造データ
-リンク
-集合体
登録構造単位 |
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-要素
#1: タンパク質 | 分子量: 41838.766 Da / 分子数: 5 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: ACTG1, ACTG / 発現宿主: Homo sapiens (ヒト) / 参照: UniProt: P63261 #2: タンパク質 | 分子量: 12180.806 Da / 分子数: 6 / Fragment: C1 Ig-domain (UNP residues 151-258) / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: MYBPC3 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q14896 #3: タンパク質 | 分子量: 10706.060 Da / 分子数: 5 / Fragment: C0 Ig-domain (UNP residues 1-101) / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 遺伝子: MYBPC3 / 発現宿主: Escherichia coli (大腸菌) / 参照: UniProt: Q14896 #4: タンパク質 | 分子量: 10826.337 Da / 分子数: 4 / 由来タイプ: 組換発現 / 由来: (組換発現) Homo sapiens (ヒト) / 発現宿主: Homo sapiens (ヒト) |
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-実験情報
-実験
実験 | 手法: 電子顕微鏡法 |
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EM実験 | 試料の集合状態: HELICAL ARRAY / 3次元再構成法: らせん対称体再構成法 |
-試料調製
構成要素 |
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分子量 | 実験値: NO | |||||||||||||||||||||||||||||||||||
由来(天然) |
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由来(組換発現) |
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緩衝液 | pH: 7 | |||||||||||||||||||||||||||||||||||
試料 | 包埋: NO / シャドウイング: NO / 染色: NO / 凍結: YES | |||||||||||||||||||||||||||||||||||
試料支持 | グリッドの材料: COPPER / グリッドのサイズ: 300 divisions/in. | |||||||||||||||||||||||||||||||||||
急速凍結 | 装置: FEI VITROBOT MARK II / 凍結剤: ETHANE / 湿度: 95 % / 凍結前の試料温度: 294 K |
-電子顕微鏡撮影
実験機器 | モデル: Titan Krios / 画像提供: FEI Company |
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顕微鏡 | モデル: FEI TITAN KRIOS |
電子銃 | 電子線源: FIELD EMISSION GUN / 加速電圧: 300 kV / 照射モード: FLOOD BEAM |
電子レンズ | モード: BRIGHT FIELD |
撮影 | 電子線照射量: 20 e/Å2 / 検出モード: INTEGRATING フィルム・検出器のモデル: FEI FALCON II (4k x 4k) |
-解析
EMソフトウェア |
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CTF補正 | タイプ: PHASE FLIPPING AND AMPLITUDE CORRECTION | ||||||||||||||||||||||||||||||||||||
らせん対称 | 回転角度/サブユニット: -166.6 ° / 軸方向距離/サブユニット: 27.5 Å / らせん対称軸の対称性: C1 | ||||||||||||||||||||||||||||||||||||
3次元再構成 | 解像度: 11 Å / 解像度の算出法: FSC 0.143 CUT-OFF / 粒子像の数: 6117 / アルゴリズム: BACK PROJECTION / 対称性のタイプ: HELICAL | ||||||||||||||||||||||||||||||||||||
原子モデル構築 | プロトコル: FLEXIBLE FIT / 空間: REAL / Target criteria: Correlation coefficient |