[English] 日本語
Yorodumi- PDB-6cjs: Candida albicans Hsp90 nucleotide binding domain in complex with ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6cjs | ||||||
---|---|---|---|---|---|---|---|
Title | Candida albicans Hsp90 nucleotide binding domain in complex with AUY922 | ||||||
Components | Heat shock protein 90 homolog | ||||||
Keywords | CHAPERONE / Hsp90. ATPase | ||||||
Function / homology | Function and homology information negative regulation of filamentous growth of a population of unicellular organisms / filamentous growth of a population of unicellular organisms / hyphal cell wall / fungal-type cell wall / filamentous growth / nuclear receptor-mediated steroid hormone signaling pathway / ATP-dependent protein folding chaperone / disordered domain specific binding / extracellular vesicle / unfolded protein binding ...negative regulation of filamentous growth of a population of unicellular organisms / filamentous growth of a population of unicellular organisms / hyphal cell wall / fungal-type cell wall / filamentous growth / nuclear receptor-mediated steroid hormone signaling pathway / ATP-dependent protein folding chaperone / disordered domain specific binding / extracellular vesicle / unfolded protein binding / protein folding / cellular response to heat / regulation of apoptotic process / protein stabilization / perinuclear region of cytoplasm / cell surface / ATP hydrolysis activity / protein-containing complex / ATP binding / membrane / plasma membrane / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | Candida albicans (yeast) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Kirkpatrick, M.G. / Pizarro, J.C. | ||||||
Funding support | United States, 1items
| ||||||
Citation | Journal: Nat Commun / Year: 2019 Title: Structural basis for species-selective targeting of Hsp90 in a pathogenic fungus. Authors: Whitesell, L. / Robbins, N. / Huang, D.S. / McLellan, C.A. / Shekhar-Guturja, T. / LeBlanc, E.V. / Nation, C.S. / Hui, R. / Hutchinson, A. / Collins, C. / Chatterjee, S. / Trilles, R. / Xie, ...Authors: Whitesell, L. / Robbins, N. / Huang, D.S. / McLellan, C.A. / Shekhar-Guturja, T. / LeBlanc, E.V. / Nation, C.S. / Hui, R. / Hutchinson, A. / Collins, C. / Chatterjee, S. / Trilles, R. / Xie, J.L. / Krysan, D.J. / Lindquist, S. / Porco Jr., J.A. / Tatu, U. / Brown, L.E. / Pizarro, J. / Cowen, L.E. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 6cjs.cif.gz | 152.5 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb6cjs.ent.gz | 119.4 KB | Display | PDB format |
PDBx/mmJSON format | 6cjs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 6cjs_validation.pdf.gz | 764.3 KB | Display | wwPDB validaton report |
---|---|---|---|---|
Full document | 6cjs_full_validation.pdf.gz | 764.4 KB | Display | |
Data in XML | 6cjs_validation.xml.gz | 11.7 KB | Display | |
Data in CIF | 6cjs_validation.cif.gz | 16.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/cj/6cjs ftp://data.pdbj.org/pub/pdb/validation_reports/cj/6cjs | HTTPS FTP |
-Related structure data
Related structure data | 6cjiSC 6cjjC 6cjlC 6cjpC 6cjrC S: Starting model for refinement C: citing same article (ref.) |
---|---|
Similar structure data |
-Links
-Assembly
Deposited unit |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||||||
Unit cell |
| ||||||||||||
Components on special symmetry positions |
|
-Components
#1: Protein | Mass: 26208.641 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida albicans (strain SC5314 / ATCC MYA-2876) (yeast) Strain: SC5314 / ATCC MYA-2876 Gene: HSP90, CAALFM_C702030WA, CaJ7.0234, CaO19.13868, CaO19.6515 Plasmid: pET15-MHL / Production host: Escherichia coli (E. coli) / Strain (production host): Rossetta 2 / References: UniProt: P46598 |
---|---|
#2: Chemical | ChemComp-2GJ / |
#3: Chemical | ChemComp-EDO / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 4 Å3/Da / Density % sol: 69.27 % |
---|---|
Crystal grow | Temperature: 298.15 K / Method: vapor diffusion, sitting drop / pH: 7.5 Details: 0.1M Hepes 0.02M MgCl2 22% Polyacrylic acid sodium salt 5100 |
-Data collection
Diffraction | Mean temperature: 120 K |
---|---|
Diffraction source | Source: SYNCHROTRON / Site: APS / Beamline: 23-ID-D / Wavelength: 1.03319 Å |
Detector | Type: DECTRIS PILATUS3 6M / Detector: PIXEL / Date: Nov 18, 2017 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.03319 Å / Relative weight: 1 |
Reflection | Resolution: 1.89→43.1 Å / Num. obs: 34601 / % possible obs: 99.9 % / Redundancy: 12.9 % / Rmerge(I) obs: 0.081 / Net I/σ(I): 16.9 |
Reflection shell | Resolution: 1.89→1.96 Å / Redundancy: 12.8 % / Mean I/σ(I) obs: 1.2 / Num. unique obs: 3371 / % possible all: 99.6 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6CJI Resolution: 1.9→43.1 Å / Cross valid method: FREE R-VALUE
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→43.1 Å
|