+データを開く
-基本情報
登録情報 | データベース: PDB / ID: 6c0a | ||||||
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タイトル | Actinin-1 EF-Hand bound to the Cav1.2 IQ Motif | ||||||
要素 |
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キーワード | SIGNALING PROTEIN / actinin-1 / EF hand / Calcium Calmodulin / Neuronal signaling / Voltage Gated Calcium Channel / Cav1.2 / Voltage Gated Calcium Channel 1.2 / Hippocampal Neurons / IQ Motif | ||||||
機能・相同性 | 機能・相同性情報 cytoskeletal regulatory protein binding / dense core granule membrane / Regulation of cytoskeletal remodeling and cell spreading by IPP complex components / structural constituent of postsynapse / RHOF GTPase cycle / RHOBTB2 GTPase cycle / RHOD GTPase cycle / voltage-gated calcium channel activity involved in AV node cell action potential / voltage-gated calcium channel activity involved in cardiac muscle cell action potential / immune system development ...cytoskeletal regulatory protein binding / dense core granule membrane / Regulation of cytoskeletal remodeling and cell spreading by IPP complex components / structural constituent of postsynapse / RHOF GTPase cycle / RHOBTB2 GTPase cycle / RHOD GTPase cycle / voltage-gated calcium channel activity involved in AV node cell action potential / voltage-gated calcium channel activity involved in cardiac muscle cell action potential / immune system development / actin filament network formation / membrane depolarization during atrial cardiac muscle cell action potential / Phase 2 - plateau phase / calcium ion transmembrane transport via high voltage-gated calcium channel / positive regulation of muscle contraction / membrane depolarization during AV node cell action potential / positive regulation of adenylate cyclase activity / Platelet degranulation / high voltage-gated calcium channel activity / cardiac conduction / L-type voltage-gated calcium channel complex / vinculin binding / cortical cytoskeleton organization / membrane depolarization during cardiac muscle cell action potential / fascia adherens / cell communication by electrical coupling involved in cardiac conduction / muscle cell development / regulation of ventricular cardiac muscle cell action potential / focal adhesion assembly / cardiac muscle cell action potential involved in contraction / camera-type eye development / NCAM1 interactions / embryonic forelimb morphogenesis / calcium ion transport into cytosol / voltage-gated calcium channel complex / platelet formation / cortical actin cytoskeleton / cortical cytoskeleton / calcium ion import across plasma membrane / Phase 0 - rapid depolarisation / alpha-actinin binding / regulation of heart rate by cardiac conduction / brush border / actin filament bundle assembly / voltage-gated calcium channel activity / regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion / stress fiber / ruffle / sarcomere / nuclear receptor coactivator activity / secretory granule / cell projection / Regulation of insulin secretion / actin filament / calcium ion transmembrane transport / postsynaptic density membrane / Z disc / Adrenaline,noradrenaline inhibits insulin secretion / double-stranded RNA binding / actin filament binding / integrin binding / cell junction / heart development / positive regulation of cytosolic calcium ion concentration / actin cytoskeleton organization / perikaryon / transmembrane transporter binding / dendritic spine / postsynaptic density / cytoskeleton / calmodulin binding / protein domain specific binding / focal adhesion / glutamatergic synapse / dendrite / calcium ion binding / protein homodimerization activity / membrane / metal ion binding / plasma membrane / cytoplasm 類似検索 - 分子機能 | ||||||
生物種 | Mus musculus (ハツカネズミ) Homo sapiens (ヒト) | ||||||
手法 | 溶液NMR / simulated annealing | ||||||
データ登録者 | Turner, M.L. / Ames, J.B. | ||||||
資金援助 | 米国, 1件
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引用 | ジャーナル: To be Published タイトル: Structural Basis of the Localization and Activation of Neuronal L-type Ca2+ Channels by a-Actinin1 and Calmodulin 著者: Turner, M.L. / Anderson, D.E. / Coleman, A. / Horn, M. / Navedo, M. / Ames, J.B. / Hell, J.W. #1: ジャーナル: Biomol NMR Assign / 年: 2016 タイトル: Chemical shift assignments of the C-terminal EF-hand domain of a-actinin-1. 著者: Turner, M. / Anderson, D.E. / Rajan, S. / Hell, J.W. / Ames, J.B. | ||||||
履歴 |
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-構造の表示
構造ビューア | 分子: MolmilJmol/JSmol |
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-ダウンロードとリンク
-ダウンロード
PDBx/mmCIF形式 | 6c0a.cif.gz | 126.1 KB | 表示 | PDBx/mmCIF形式 |
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PDB形式 | pdb6c0a.ent.gz | 99.8 KB | 表示 | PDB形式 |
PDBx/mmJSON形式 | 6c0a.json.gz | ツリー表示 | PDBx/mmJSON形式 | |
その他 | その他のダウンロード |
-検証レポート
文書・要旨 | 6c0a_validation.pdf.gz | 467.2 KB | 表示 | wwPDB検証レポート |
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文書・詳細版 | 6c0a_full_validation.pdf.gz | 525.2 KB | 表示 | |
XML形式データ | 6c0a_validation.xml.gz | 13.2 KB | 表示 | |
CIF形式データ | 6c0a_validation.cif.gz | 17.5 KB | 表示 | |
アーカイブディレクトリ | https://data.pdbj.org/pub/pdb/validation_reports/c0/6c0a ftp://data.pdbj.org/pub/pdb/validation_reports/c0/6c0a | HTTPS FTP |
-関連構造データ
類似構造データ | |
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その他のデータベース |
-リンク
-集合体
登録構造単位 |
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1 |
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NMR アンサンブル |
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-要素
#1: タンパク質 | 分子量: 7848.695 Da / 分子数: 1 / 断片: EF-hand 3,4 (UNP residues 822-892) / 由来タイプ: 組換発現 / 由来: (組換発現) Mus musculus (ハツカネズミ) / 遺伝子: Actn1 発現宿主: Escherichia coli 'BL21-Gold(DE3)pLysS AG' (大腸菌) 参照: UniProt: Q7TPR4 |
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#2: タンパク質・ペプチド | 分子量: 2920.431 Da / 分子数: 1 / 断片: IQ Motif (UNP residues 1587-1609) / 由来タイプ: 合成 / 由来: (合成) Homo sapiens (ヒト) / 参照: UniProt: Q13936 |
-実験情報
-実験
実験 | 手法: 溶液NMR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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NMR実験 |
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-試料調製
詳細 |
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試料 |
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試料状態 | 詳細: All samples were performed with the following buffer system: 20 mM Tris-d11 (pH 7.5) with 1 mM DTT d10, 1 mM EDTA-d12 and 95 % H2O/5 % D2O イオン強度: 0 mM / Label: Sample Conditions all Samples / pH: 7.5 / 圧: 1 atm / 温度: 303 K |
-NMR測定
NMRスペクトロメーター |
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-解析
NMR software |
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精密化 | 手法: simulated annealing / ソフトェア番号: 2 / 詳細: also distance geometry and torsion angle dynamics | |||||||||||||||||||||
代表構造 | 選択基準: none | |||||||||||||||||||||
NMRアンサンブル | コンフォーマー選択の基準: structures with the lowest energy 計算したコンフォーマーの数: 194 / 登録したコンフォーマーの数: 4 |