+Open data
-Basic information
Entry | Database: PDB / ID: 5a15 | |||||||||
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Title | Crystal structure of the BTB domain of human KCTD16 | |||||||||
Components | BTB/POZ DOMAIN-CONTAINING PROTEIN KCTD16 | |||||||||
Keywords | SIGNALING PROTEIN | |||||||||
Function / homology | Function and homology information regulation of G protein-coupled receptor signaling pathway / cell projection / protein homooligomerization / presynaptic membrane / postsynaptic membrane / receptor complex Similarity search - Function | |||||||||
Biological species | HOMO SAPIENS (human) | |||||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.76 Å | |||||||||
Authors | Pinkas, D.M. / Sanvitale, C.E. / Solcan, N. / Goubin, S. / Canning, P. / Dixon Clarke, S.E. / Talon, R. / Wiggers, H.J. / Fitzpatrick, F. / Tallant, C. ...Pinkas, D.M. / Sanvitale, C.E. / Solcan, N. / Goubin, S. / Canning, P. / Dixon Clarke, S.E. / Talon, R. / Wiggers, H.J. / Fitzpatrick, F. / Tallant, C. / Kopec, J. / Chalk, R. / Doutch, J. / Krojer, T. / Burgess-Brown, N.A. / von Delft, F. / Arrowsmith, C.H. / Edwards, A.M. / Bountra, C. / Bullock, A. | |||||||||
Citation | Journal: Biochem. J. / Year: 2017 Title: Structural complexity in the KCTD family of Cullin3-dependent E3 ubiquitin ligases. Authors: Pinkas, D.M. / Sanvitale, C.E. / Bufton, J.C. / Sorrell, F.J. / Solcan, N. / Chalk, R. / Doutch, J. / Bullock, A.N. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 5a15.cif.gz | 541 KB | Display | PDBx/mmCIF format |
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PDB format | pdb5a15.ent.gz | 456.7 KB | Display | PDB format |
PDBx/mmJSON format | 5a15.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/a1/5a15 ftp://data.pdbj.org/pub/pdb/validation_reports/a1/5a15 | HTTPS FTP |
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-Related structure data
Related structure data | 4crhC 4uijC 5a6rC 5ftaC 4ues C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
-Components
#1: Protein | Mass: 13962.897 Da / Num. of mol.: 15 / Fragment: BTB DOMAIN, UNP RESIDUES 16-133 Source method: isolated from a genetically manipulated source Source: (gene. exp.) HOMO SAPIENS (human) / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21 / References: UniProt: Q68DU8 |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 3.1 Å3/Da / Density % sol: 60.3 % / Description: NONE |
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Crystal grow | pH: 6 / Details: 21% PEG3350, 0.1M BIS-TRIS PH 6.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: Diamond / Beamline: I03 / Wavelength: 0.97623 |
Detector | Type: DECTRIS PIXEL / Detector: PIXEL / Date: Apr 25, 2014 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97623 Å / Relative weight: 1 |
Reflection | Resolution: 2.76→36.55 Å / Num. obs: 50928 / % possible obs: 97.9 % / Observed criterion σ(I): -3 / Redundancy: 3.5 % / Biso Wilson estimate: 81.92 Å2 / Rmerge(I) obs: 0.05 / Net I/σ(I): 13.7 |
Reflection shell | Resolution: 2.76→2.83 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.65 / Mean I/σ(I) obs: 2.1 / % possible all: 98.2 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 4UES 4ues Resolution: 2.76→34.65 Å / SU ML: 0.39 / σ(F): 1.96 / Phase error: 33.61 / Stereochemistry target values: ML
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 115.9 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.76→34.65 Å
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Refine LS restraints |
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LS refinement shell |
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