OXIDOREDUCTASE / PROTEIN ENGINEERING: 4-HYDROXYPHENYLACETATE 3-MONOOXYGENASE / OXYGENASE COMPONENT / OXIDOREDUCTASE ACTIVE SITE LOOP MUTANT 7.2
機能・相同性
機能・相同性情報
oxidoreductase activity, acting on the CH-CH group of donors / phenylacetate catabolic process / oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen / flavin adenine dinucleotide binding 類似検索 - 分子機能
マシュー密度: 3.66 Å3/Da / 溶媒含有率: 66.42 % 解説: Crystal is a clear colorless prism measuring 80 BY 50 BY 10 microns
結晶化
温度: 291 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 7 詳細: HANGING DROP VAPOR DIFFUSION AT 291K USING 2 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN SOLUTION AND A WELL SOLUTION CONTAINING 1.0 M SUCCINIC ACID, 0.1 M HEPES, AND 1% W/V ...詳細: HANGING DROP VAPOR DIFFUSION AT 291K USING 2 MICROLITER DROPS CONTAINING EQUAL VOLUMES OF PROTEIN SOLUTION AND A WELL SOLUTION CONTAINING 1.0 M SUCCINIC ACID, 0.1 M HEPES, AND 1% W/V POLYETHYLENE GLYCOL MONOMETHYL ETHER 2,000 AT PH 7.0. CRYOPROTECTANT WAS MADE BY ADDING 30 % (V/V) GLYCEROL TO THE PRECIPITANT COCKTAIL. PH範囲: 7