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- PDB-6avm: STRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WIT... -

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Basic information

Entry
Database: PDB / ID: 6avm
TitleSTRUCTURE OF HIV-1 REVERSE TRANSCRIPTASE (RT) TERNARY COMPLEX WITH A DOUBLE STRANDED DNA AND AN INCOMING D4TTP AT PH 9.5 WITH CROSS-LINKING TO SECOND BASE TEMPLATE OVERHANG
Components
  • (HIV-1 REVERSE TRANSCRIPTASE ...) x 2
  • DNA (27-MER)
  • DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*(DDG))-3')
KeywordsTRANSFERASE/DNA / RT / DNA / CROSSLINK / N SITE COMPLEX / D4T (STAVUDINE) / PYROPHOSPHOROLYSIS / P51 / P66 / TRANSFERASE-DNA COMPLEX / VIRAL PROTEIN / TRANSCRIPTION-DNA complex
Function / homology
Function and homology information


HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency ...HIV-1 retropepsin / symbiont-mediated activation of host apoptosis / retroviral ribonuclease H / exoribonuclease H / exoribonuclease H activity / host multivesicular body / DNA integration / viral genome integration into host DNA / RNA-directed DNA polymerase / establishment of integrated proviral latency / symbiont-mediated suppression of host gene expression / viral penetration into host nucleus / RNA stem-loop binding / RNA-directed DNA polymerase activity / RNA-DNA hybrid ribonuclease activity / Transferases; Transferring phosphorus-containing groups; Nucleotidyltransferases / host cell / viral nucleocapsid / DNA recombination / DNA-directed DNA polymerase / Hydrolases; Acting on ester bonds / aspartic-type endopeptidase activity / DNA-directed DNA polymerase activity / symbiont entry into host cell / viral translational frameshifting / lipid binding / host cell nucleus / host cell plasma membrane / structural molecule activity / virion membrane / proteolysis / DNA binding / zinc ion binding / membrane
Similarity search - Function
HIV Type 1 Reverse Transcriptase, subunit A, domain 1 / HIV Type 1 Reverse Transcriptase; Chain A, domain 1 / Reverse transcriptase/Diguanylate cyclase domain / Ribonuclease H-like superfamily/Ribonuclease H / Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. ...HIV Type 1 Reverse Transcriptase, subunit A, domain 1 / HIV Type 1 Reverse Transcriptase; Chain A, domain 1 / Reverse transcriptase/Diguanylate cyclase domain / Ribonuclease H-like superfamily/Ribonuclease H / Reverse transcriptase connection / Reverse transcriptase connection domain / Reverse transcriptase thumb / Reverse transcriptase thumb domain / Integrase Zinc binding domain / Zinc finger integrase-type profile. / Integrase-like, N-terminal / Integrase DNA binding domain / Integrase, C-terminal domain superfamily, retroviral / Integrase, N-terminal zinc-binding domain / Integrase, C-terminal, retroviral / Integrase DNA binding domain profile. / Immunodeficiency lentiviral matrix, N-terminal / gag gene protein p17 (matrix protein) / RNase H / Integrase core domain / Integrase, catalytic core / Integrase catalytic domain profile. / Retropepsin-like catalytic domain / RNase H type-1 domain profile. / Ribonuclease H domain / Matrix protein, lentiviral and alpha-retroviral, N-terminal / Retroviral nucleocapsid Gag protein p24, C-terminal domain / Gag protein p24 C-terminal domain / Reverse transcriptase domain / Reverse transcriptase (RT) catalytic domain profile. / Retropepsins / Retroviral aspartyl protease / Aspartyl protease, retroviral-type family profile. / Peptidase A2A, retrovirus, catalytic / Reverse transcriptase (RNA-dependent DNA polymerase) / Retroviral matrix protein / Retrovirus capsid, C-terminal / Retrovirus capsid, N-terminal / zinc finger / Zinc knuckle / Zinc finger, CCHC-type superfamily / Zinc finger, CCHC-type / Zinc finger CCHC-type profile. / Nucleotidyltransferase; domain 5 / Aspartic peptidase, active site / Eukaryotic and viral aspartyl proteases active site. / Ribonuclease H superfamily / Aspartic peptidase domain superfamily / Ribonuclease H-like superfamily / Reverse transcriptase/Diguanylate cyclase domain / Alpha-Beta Plaits / Roll / DNA/RNA polymerase superfamily / 2-Layer Sandwich / Alpha Beta
Similarity search - Domain/homology
Chem-D4T / DNA / DNA (> 10) / Gag-Pol polyprotein
Similarity search - Component
Biological speciesHuman immunodeficiency virus type 1 group M subtype B
Human immunodeficiency virus type 1 BH10
Homo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.502 Å
AuthorsMartinez, S.E. / Das, K. / Arnold, E.
Funding support United States, 1items
OrganizationGrant numberCountry
National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)R37 A1027690 United States
CitationJournal: Protein Sci. / Year: 2019
Title: Structure of HIV-1 reverse transcriptase/d4TTP complex: Novel DNA cross-linking site and pH-dependent conformational changes.
Authors: Martinez, S.E. / Bauman, J.D. / Das, K. / Arnold, E.
History
DepositionSep 3, 2017Deposition site: RCSB / Processing site: RCSB
Revision 1.0Aug 15, 2018Provider: repository / Type: Initial release
Revision 1.1Dec 19, 2018Group: Data collection / Database references / Derived calculations
Category: citation / citation_author ...citation / citation_author / struct_site / struct_site_gen
Item: _citation.country / _citation.journal_abbrev ..._citation.country / _citation.journal_abbrev / _citation.journal_id_ASTM / _citation.journal_id_CSD / _citation.journal_id_ISSN / _citation.pdbx_database_id_DOI / _citation.pdbx_database_id_PubMed / _citation.title / _citation.year
Revision 1.2Feb 20, 2019Group: Data collection / Database references / Category: citation / citation_author
Item: _citation.journal_volume / _citation.page_first ..._citation.journal_volume / _citation.page_first / _citation.page_last / _citation.title / _citation.year / _citation_author.identifier_ORCID
Revision 1.3Dec 11, 2019Group: Author supporting evidence / Category: pdbx_audit_support / Item: _pdbx_audit_support.funding_organization
Revision 1.4Oct 4, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_conn_type
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.conn_type_id / _struct_conn.id / _struct_conn.pdbx_dist_value / _struct_conn.pdbx_leaving_atom_flag / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_conn_type.id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT
B: HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT
T: DNA (27-MER)
P: DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*(DDG))-3')
C: HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT
D: HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT
E: DNA (27-MER)
F: DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*(DDG))-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)263,61826
Polymers261,4878
Non-polymers2,13118
Water11,908661
1
A: HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT
B: HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT
T: DNA (27-MER)
P: DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*(DDG))-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)131,90114
Polymers130,7444
Non-polymers1,15710
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area15650 Å2
ΔGint-61 kcal/mol
Surface area47800 Å2
MethodPISA
2
C: HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT
D: HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT
E: DNA (27-MER)
F: DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*(DDG))-3')
hetero molecules


Theoretical massNumber of molelcules
Total (without water)131,71712
Polymers130,7444
Non-polymers9738
Water724
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Buried area14850 Å2
ΔGint-64 kcal/mol
Surface area48530 Å2
MethodPISA
Unit cell
Length a, b, c (Å)90.264, 133.333, 140.203
Angle α, β, γ (deg.)90.000, 97.630, 90.000
Int Tables number4
Space group name H-MP1211

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Components

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HIV-1 REVERSE TRANSCRIPTASE ... , 2 types, 4 molecules ACBD

#1: Protein HIV-1 REVERSE TRANSCRIPTASE P66 SUBUNIT / Pr160Gag-Pol


Mass: 64038.367 Da / Num. of mol.: 2 / Mutation: C280S, I63C
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 group M subtype B (isolate BH10)
Strain: isolate BH10 / Gene: gag-pol / Plasmid: pCDF-2 Ek/LIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): BL21 CodonPlus RIL
References: UniProt: P03366, RNA-directed DNA polymerase, DNA-directed DNA polymerase
#2: Protein HIV-1 REVERSE TRANSCRIPTASE P51 SUBUNIT / Pr160Gag-Pol


Mass: 51928.629 Da / Num. of mol.: 2 / Mutation: C879S
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Human immunodeficiency virus type 1 group M subtype B (isolate BH10)
Strain: isolate BH10 / Gene: gag-pol / Plasmid: pCDF-2 Ek/LIC / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): BL21 CodonPlus RIL
References: UniProt: P03366, RNA-directed DNA polymerase, DNA-directed DNA polymerase

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DNA chain , 2 types, 4 molecules TEPF

#3: DNA chain DNA (27-MER)


Mass: 8376.399 Da / Num. of mol.: 2 / Source method: obtained synthetically
Details: COMMERCIAL DNA OLIGO SYNTHESIS: INTEGRATED DNA TECHNOLOGIES
Source: (synth.) Human immunodeficiency virus type 1 BH10
#4: DNA chain DNA (5'-D(*A*CP*AP*GP*TP*CP*CP*CP*TP*GP*TP*TP*CP*GP*GP*GP*CP*GP*CP*CP*(DDG))-3')


Mass: 6400.123 Da / Num. of mol.: 2 / Source method: obtained synthetically
Details: COMMERCIAL DNA OLIGO SYNTHESIS: MIDLAND CERTIFIED REAGENT COMPANY
Source: (synth.) Homo sapiens (human)

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Non-polymers , 4 types, 679 molecules

#5: Chemical
ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 4 / Source method: obtained synthetically / Formula: Mg
#6: Chemical ChemComp-D4T / 2',3'-DEHYDRO-2',3'-DEOXY-THYMIDINE 5'-TRIPHOSPHATE


Mass: 464.153 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: C10H15N2O13P3
#7: Chemical
ChemComp-GOL / GLYCEROL / GLYCERIN / PROPANE-1,2,3-TRIOL


Mass: 92.094 Da / Num. of mol.: 12 / Source method: obtained synthetically / Formula: C3H8O3
#8: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 661 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 3.2 Å3/Da / Density % sol: 61.54 % / Description: PARALLELOGRAMS
Crystal growTemperature: 277 K / Method: vapor diffusion, sitting drop / pH: 9.5
Details: PEG 8000, NaCl, CHES (N-Cyclohexyl-2-aminoethanesulfonic acid), TRIS, MgCl2, d4T triphosphate
PH range: 9.5 - 10.5

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: CHESS / Beamline: F1 / Wavelength: 0.9186 Å
DetectorType: ADSC QUANTUM 270 / Detector: CCD / Date: Jun 10, 2010
Details: White beam collimating mirror, horizontally focusing monochromator using single bent triangular Si(111) crystal, vertically focusing Rh-coated Si mirror
RadiationMonochromator: Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 0.9186 Å / Relative weight: 1
ReflectionResolution: 2.5→50 Å / Num. obs: 112162 / % possible obs: 99.3 % / Redundancy: 6.6 % / Biso Wilson estimate: 54.62 Å2 / Rmerge(I) obs: 0.101 / Χ2: 1.087 / Net I/σ(I): 17
Reflection shell

Diffraction-ID: 1

Resolution (Å)Redundancy (%)Rmerge(I) obsMean I/σ(I) obsNum. unique obsΧ2% possible all
2.5-2.595.20.7851.8111910.99599.7
2.59-2.695.80.6872.4112431.015100
2.69-2.826.20.563.2112611.069100
2.82-2.966.40.4174.5112331.131100
2.96-3.156.60.2837.1112741.195100
3.15-3.396.90.17711.8113111.215100
3.39-3.736.90.11816.8112701.22199.9
3.73-4.277.30.07325112781.10299.9
4.27-5.387.40.05629113190.96899.8
5.38-507.10.04629.8107820.93194

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Phasing

PhasingMethod: molecular replacement
Phasing MR
Highest resolutionLowest resolution
Rotation8.61 Å34.37 Å
Translation8.61 Å34.37 Å

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Processing

Software
NameVersionClassification
HKL-2000data reduction
HKL-2000data scaling
PHASER2.5.6phasing
PHENIX1.11.1 2575refinement
PDB_EXTRACT3.22data extraction
Coot0.8.8model building
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: PDBID 6AMO

6amo


Resolution: 2.502→34.372 Å / SU ML: 0.36 / Cross valid method: FREE R-VALUE / σ(F): 1.36 / Phase error: 25.05
RfactorNum. reflection% reflection
Rfree0.2291 2003 1.79 %
Rwork0.191 --
obs0.1916 112126 98.91 %
Solvent computationShrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å
Displacement parametersBiso max: 295.43 Å2 / Biso mean: 92.9455 Å2 / Biso min: 19.18 Å2
Refinement stepCycle: final / Resolution: 2.502→34.372 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms15778 1716 132 661 18287
Biso mean--94.31 64.96 -
Num. residues----2008
Refine LS restraints
Refine-IDTypeDev idealNumber
X-RAY DIFFRACTIONf_bond_d0.00618270
X-RAY DIFFRACTIONf_angle_d0.44325129
X-RAY DIFFRACTIONf_chiral_restr0.042722
X-RAY DIFFRACTIONf_plane_restr0.0032859
X-RAY DIFFRACTIONf_dihedral_angle_d12.93910627
LS refinement shell

Refine-ID: X-RAY DIFFRACTION / Rfactor Rfree error: 0 / Total num. of bins used: 14

Resolution (Å)Rfactor RfreeNum. reflection RfreeRfactor RworkNum. reflection RworkNum. reflection all% reflection obs (%)
2.502-2.56450.34371330.31637435756894
2.5645-2.63380.33351490.299679118060100
2.6338-2.71130.37441450.286178998044100
2.7113-2.79880.32911450.263779238068100
2.7988-2.89870.2571470.244679508097100
2.8987-3.01470.2951390.231979248063100
3.0147-3.15190.26441460.212879048050100
3.1519-3.31790.2671440.205479428086100
3.3179-3.52560.22931400.193779298069100
3.5256-3.79750.20031440.186679728116100
3.7975-4.1790.20271460.156679348080100
4.179-4.78240.16891430.147379728115100
4.7824-6.020.18421450.164679898134100
6.02-34.3750.23121370.17597439757692
Refinement TLS params.

Method: refined / Refine-ID: X-RAY DIFFRACTION

IDL11 (°2)L12 (°2)L13 (°2)L22 (°2)L23 (°2)L33 (°2)S11 (Å °)S12 (Å °)S13 (Å °)S21 (Å °)S22 (Å °)S23 (Å °)S31 (Å °)S32 (Å °)S33 (Å °)T11 (Å2)T12 (Å2)T13 (Å2)T22 (Å2)T23 (Å2)T33 (Å2)Origin x (Å)Origin y (Å)Origin z (Å)
1-0.08540.04090.00930.0089-0.17220.57110.11710.09430.5321-0.52890.20260.5662-0.4462-0.5288-0.2071.5627-0.2463-0.07951.04220.16271.016331.880923.072822.8859
21.2850.1472-0.47761.4736-0.35092.70090.20820.36780.6674-0.51630.15120.1172-0.68660.2188-0.25481.1934-0.29050.04950.67860.0730.827440.839525.216938.7352
30.99011.2146-0.53541.7861-0.44762.270.0350.06270.7272-0.2340.16910.4699-0.7687-0.1497-0.10570.5766-0.0306-0.03890.3947-0.0860.731824.571719.711862.8318
43.39661.0802-1.23121.3125-0.25871.6622-0.10820.10390.0514-0.04490.16470.2223-0.1136-0.2283-0.01260.31790.0517-0.06750.4114-0.06270.45316.8409-2.05573.7053
51.57520.9679-0.29323.7396-0.06771.9944-0.22360.3176-0.3997-0.71210.2952-0.49270.06390.5958-0.05030.5994-0.11240.10950.7692-0.22770.621342.2918-14.334442.1017
61.3424-1.2717-1.94121.70771.29162.5858-0.7834-0.1475-0.60510.25780.11370.23671.01710.11910.65420.67040.0280.12840.5455-0.05120.789914.2267-27.795468.8178
71.88671.25110.70412.79451.55713.4172-0.1759-0.145-0.1001-0.1025-0.03350.18120.1805-0.00870.17160.332-0.0208-0.01460.4058-0.12040.521125.8688-15.305158.7999
80.1526-0.14830.35881.7181-0.85291.26830.46780.1782-0.2470.0465-0.05420.3905-0.1125-0.3868-0.47821.69290.12020.10131.0192-0.08410.841922.7839-61.502-13.9734
90.06380.14790.29080.5209-0.3681.0691-0.23410.4335-0.0313-0.74080.23070.60730.416-0.7619-0.04111.8751-0.0614-0.19011.42240.00350.918316.2754-53.9041-12.8854
101.11470.1521-0.71990.6135-0.32822.88650.4020.82010.3171-0.8275-0.03570.2149-0.417-0.7379-0.21381.60170.19020.03761.05930.10180.704524.0078-40.7346-5.0713
111.0826-0.2763-0.59811.4138-0.50863.68980.24170.50980.3159-0.48780.03650.296-0.4244-0.7957-0.17360.71550.0432-0.03960.53230.00390.556111.1075-41.670822.7071
121.55170.7154-0.41112.2174-0.61322.0108-0.0485-0.2774-0.1231-0.18670.09970.0434-0.0312-0.2064-0.04180.2677-0.0105-0.05440.4663-0.05720.428311.5964-59.545347.797
133.10920.14620.42133.06010.53882.29670.02450.15350.0024-0.7639-0.1722-0.38850.3591.0906-0.01561.09380.11250.25040.85210.02080.630542.2025-56.913511.3567
141.0174-0.3452-0.24682.325-0.32231.60990.19120.0885-0.0553-0.7228-0.3849-0.67320.49811.28730.05181.03660.30350.37771.27870.11650.840652.039-65.862819.0682
150.4048-0.00920.13970.5092-0.61330.4082-0.0870.1499-0.0555-0.3646-0.1111-0.37450.15880.61570.10150.46720.00660.09470.92930.0680.691237.3869-69.442850.3541
161.149-0.423-0.37451.7979-1.60073.67140.048-0.0918-0.2942-0.4877-0.3051-0.20930.51030.49320.21650.43340.023-0.00450.5541-0.03820.536133.5764-72.530948.3511
172.896-0.66970.79144.8138-0.30324.05630.118-0.2508-0.1239-0.3468-0.42230.28720.38260.32970.2130.64240.04980.08250.7173-0.06250.491333.2748-62.36233.2371
184.87933.4465-0.85734.86340.40779.63740.37611.65371.7906-0.22570.63552.4033-0.9007-0.9738-0.59451.5574-0.0587-0.15271.26170.42331.551726.322621.469434.6652
195.8912.01231.58653.0409-0.09951.0174-0.6270.6902-0.246-1.15730.44850.50510.7372-0.4090.05811.1475-0.2794-0.05530.9275-0.00230.970110.39673.711950.5269
201.37890.60681.12510.51180.18321.2995-0.14060.1152-0.1542-0.4718-0.3595-0.0879-0.0361-0.59450.47212.1175-1.0143-0.01952.5485-0.45252.6182-11.6661-13.711552.316
215.4401-0.03240.6975.20123.73627.96670.84850.6123-1.7679-1.6364-0.2701-0.01861.4351-1.0504-0.32512.1352-0.498-0.28461.9474-0.23791.3511-4.2577-12.076947.6288
221.3972.0528-0.08873.05630.19610.1441-0.69620.66010.5885-1.54820.68760.3414-0.0375-0.4860.0391.1658-0.2666-0.07611.04690.20481.071715.560711.552548.3354
231.86080.5514-1.76028.03150.94915.8978-0.65611.1542-0.2693-0.28671.05230.8870.0402-1.3045-0.35221.708-0.0119-0.01971.40420.04730.886714.0677-53.8458-1.023
241.5406-0.3377-0.42823.4703-0.92931.7093-0.06980.6789-0.6606-1.50270.32930.78671.7191-0.1301-0.20871.6553-0.2001-0.0830.9402-0.02620.973311.5655-60.237618.2279
258.0312-5.2849-5.15156.74065.5834.74440.72530.1024-1.48260.7671-0.4562-1.04711.62250.0655-0.20621.5422-0.10510.10941.45870.35861.823313.9735-80.023337.5105
262.9151-0.03180.3631.27270.01681.0149-0.9936-0.3253-0.983-0.17770.817-0.09291.14411.34890.00832.6709-0.24650.6231.7713-0.47642.087813.4818-85.825633.2709
270.2275-0.83730.73792.761-3.04292.97210.27930.7633-0.1574-2.0291-0.04080.55211.425-0.4495-0.30371.5268-0.0855-0.08261.1329-0.02080.802711.85-59.208617.6703
Refinement TLS group
IDRefine-IDRefine TLS-IDSelection detailsAuth asym-IDAuth seq-ID
1X-RAY DIFFRACTION1chain 'A' and (resid -1 through 96 )A-1 - 96
2X-RAY DIFFRACTION2chain 'A' and (resid 97 through 269 )A97 - 269
3X-RAY DIFFRACTION3chain 'A' and (resid 270 through 382 )A270 - 382
4X-RAY DIFFRACTION4chain 'A' and (resid 383 through 553 )A383 - 553
5X-RAY DIFFRACTION5chain 'B' and (resid 4 through 210 )B4 - 210
6X-RAY DIFFRACTION6chain 'B' and (resid 211 through 319 )B211 - 319
7X-RAY DIFFRACTION7chain 'B' and (resid 320 through 428 )B320 - 428
8X-RAY DIFFRACTION8chain 'C' and (resid -1 through 27 )C-1 - 27
9X-RAY DIFFRACTION9chain 'C' and (resid 28 through 96 )C28 - 96
10X-RAY DIFFRACTION10chain 'C' and (resid 97 through 253 )C97 - 253
11X-RAY DIFFRACTION11chain 'C' and (resid 254 through 382 )C254 - 382
12X-RAY DIFFRACTION12chain 'C' and (resid 383 through 553 )C383 - 553
13X-RAY DIFFRACTION13chain 'D' and (resid 4 through 71 )D4 - 71
14X-RAY DIFFRACTION14chain 'D' and (resid 72 through 210 )D72 - 210
15X-RAY DIFFRACTION15chain 'D' and (resid 211 through 269 )D211 - 269
16X-RAY DIFFRACTION16chain 'D' and (resid 270 through 382 )D270 - 382
17X-RAY DIFFRACTION17chain 'D' and (resid 383 through 428 )D383 - 428
18X-RAY DIFFRACTION18chain 'T' and (resid 704 through 708 )T704 - 708
19X-RAY DIFFRACTION19chain 'T' and (resid 709 through 723 )T709 - 723
20X-RAY DIFFRACTION20chain 'T' and (resid 724 through 725 )T724 - 725
21X-RAY DIFFRACTION21chain 'P' and (resid 803 through 807 )P803 - 807
22X-RAY DIFFRACTION22chain 'P' and (resid 808 through 821 )P808 - 821
23X-RAY DIFFRACTION23chain 'E' and (resid 704 through 708 )E704 - 708
24X-RAY DIFFRACTION24chain 'E' and (resid 709 through 718 )E709 - 718
25X-RAY DIFFRACTION25chain 'E' and (resid 719 through 725 )E719 - 725
26X-RAY DIFFRACTION26chain 'F' and (resid 803 through 807 )F803 - 807
27X-RAY DIFFRACTION27chain 'F' and (resid 808 through 821 )F808 - 821

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