A: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') B: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') C: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') D: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') E: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') F: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') G: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3') H: DNA (5'-D(*CP*GP*CP*GP*CP*G)-3')
Mass: 18.015 Da / Num. of mol.: 41 / Source method: isolated from a natural source / Formula: H2O
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Experimental details
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Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
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Sample preparation
Crystal
Density Matthews: 1.52 Å3/Da / Density % sol: 45.19 %
Crystal grow
Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 80 mM KCl, 20 mM MgCl2, 40 mM Na cacodylate pH 6.0, 12 mM spermine, 45% MPD
Resolution: 1.55→30.54 Å / Cor.coef. Fo:Fc: 0.972 / Cor.coef. Fo:Fc free: 0.976 / SU B: 6.111 / SU ML: 0.091 / Cross valid method: THROUGHOUT / ESU R: 0.037 / ESU R Free: 0.021 / Details: HYDROGENS HAVE BEEN USED IF PRESENT IN THE INPUT
Rfactor
Num. reflection
% reflection
Selection details
Rfree
0.22682
657
5 %
RANDOM
Rwork
0.20877
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obs
0.2097
12475
99.21 %
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Solvent computation
Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å