response to biotic stimulus / abscisic acid binding / abscisic acid-activated signaling pathway / protein phosphatase inhibitor activity / defense response / signaling receptor activity Similarity search - Function
Pathogenesis-related proteins Bet v I family signature. / Bet v I type allergen / Bet v I/Major latex protein / Pathogenesis-related protein Bet v 1 family / START domain / Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4 / START-like domain superfamily / 2-Layer Sandwich / Alpha Beta Similarity search - Domain/homology
Mass: 17250.387 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Fagus sylvatica (European beech) / Plasmid: pET28b / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / Variant (production host): Star / References: UniProt: B7TWE6
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Sample state
Spectrometer-ID
Type
1
1
1
isotropic
1
2D 1H-15N HSQC
1
2
1
isotropic
1
2D 1H-13C HSQC aliphatic
1
3
1
isotropic
2
2D 1H-13C HSQC aromatic
1
4
1
isotropic
3
3DCBCA(CO)NH
1
5
1
isotropic
3
3D HN(CA)CB
1
7
1
isotropic
3
3D HNCA
1
6
1
isotropic
3
3D HNCO
1
10
1
isotropic
2
3DHBHA(CO)NH
1
9
1
isotropic
2
3DHN(CO)CA
1
8
1
isotropic
2
3D (H)CCH-TOCSY
1
12
1
isotropic
2
3D 1H-15N NOESY
1
11
1
isotropic
2
3D (H)CCH-COSY
1
14
1
isotropic
2
3D 1H-13C NOESY aliphatic
1
13
1
isotropic
1
3D 1H-13C NOESY aromatic
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Sample preparation
Details
Type: solution Contents: 600 uM [U-99% 13C; U-99% 15N] Fag s 1, 10 mM sodium phosphate, 150 mM sodium chloride, 5 % [U-100% 2H] TFE, 10 % [U-100% 2H] D2O, 90% H2O/10% D2O Details: 600 micromolar of isotopically labeled (15N, 13C) Fag s 1 in 10 mM of sodium phosphate, 150 mM NaCl pH 7.8 containing 5 % of deuterated 2,2,2 trifluoroethanol-D2 (TFE) and 10 % of D2O. Label: 15N_13C_sample / Solvent system: 90% H2O/10% D2O
Sample
Conc. (mg/ml)
Component
Isotopic labeling
Solution-ID
600uM
Fags1
[U-99% 13C; U-99% 15N]
1
10mM
sodiumphosphate
naturalabundance
1
150mM
sodiumchloride
naturalabundance
1
5 %
TFE
[U-100% 2H]
1
10 %
D2O
[U-100% 2H]
1
Sample conditions
Details: 600 micromolar of isotopically labeled (15N, 13C) Fag s 1 in 10 mM of sodium phosphate, 150 mM NaCl pH 7.8 containing 5 % of deuterated 2,2,2 trifluoroethanol-D2 (TFE) and 10 % of D2O. The ...Details: 600 micromolar of isotopically labeled (15N, 13C) Fag s 1 in 10 mM of sodium phosphate, 150 mM NaCl pH 7.8 containing 5 % of deuterated 2,2,2 trifluoroethanol-D2 (TFE) and 10 % of D2O. The experiments were acquired at 308 K. Ionic strength: 0.187 M / Label: condition 1 / pH: 7.8 / PH err: 0.1 / Pressure: 1 atm / Pressure err: 0.1 / Temperature: 308 K / Temperature err: 1
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