Single alpha-helices involved in coiled-coils or other helix-helix interfaces - #80 / Phage major coat protein, Gp8 / Bacteriophage M13, G8P, capsid domain superfamily / Capsid protein G8P / Single alpha-helices involved in coiled-coils or other helix-helix interfaces / Up-down Bundle / Mainly Alpha 類似検索 - ドメイン・相同性
National Basic Research Program of China (973 Program)
2015CB910102
中国
National Natural Science Foundation of China
31470721
中国
引用
ジャーナル: Proc Natl Acad Sci U S A / 年: 2019 タイトル: Cryo-electron microscopy structure of the filamentous bacteriophage IKe. 著者: Jingwei Xu / Nir Dayan / Amir Goldbourt / Ye Xiang / 要旨: The filamentous bacteriophage IKe infects cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 Å. The major ...The filamentous bacteriophage IKe infects cells bearing IncN pili. We report the cryo-electron microscopy structure of the micrometer-long IKe viral particle at a resolution of 3.4 Å. The major coat protein [protein 8 (p8)] consists of 47 residues that fold into a ∼68-Å-long helix. An atomic model of the coat protein was built. Five p8 helices in a horizontal layer form a pentamer, and symmetrically neighboring p8 layers form a right-handed helical cylinder having a rise per pentamer of 16.77 Å and a twist of 38.52°. The inner surface of the capsid cylinder is positively charged and has direct interactions with the encapsulated circular single-stranded DNA genome, which has an electron density consistent with an unusual left-handed helix structure. Similar to capsid structures of other filamentous viruses, strong capsid packing in the IKe particle is maintained by hydrophobic residues. Despite having a different length and large sequence differences from other filamentous phages, π-π interactions were found between Tyr9 of one p8 and Trp29 of a neighboring p8 in IKe that are similar to interactions observed in phage M13, suggesting that, despite sequence divergence, overall structural features are maintained.
A: major coat protein p8 B: major coat protein p8 C: major coat protein p8 D: major coat protein p8 E: major coat protein p8 F: major coat protein p8 G: major coat protein p8 H: major coat protein p8 I: major coat protein p8 J: major coat protein p8 K: major coat protein p8 L: major coat protein p8 M: major coat protein p8 N: major coat protein p8 O: major coat protein p8 P: major coat protein p8 Q: major coat protein p8 R: major coat protein p8 S: major coat protein p8 T: major coat protein p8 U: major coat protein p8 V: major coat protein p8 W: major coat protein p8 X: major coat protein p8 Y: major coat protein p8 Z: major coat protein p8 a: major coat protein p8 b: major coat protein p8 c: major coat protein p8 d: major coat protein p8