温度: 300 K / 手法: 蒸気拡散法, ハンギングドロップ法 / pH: 4.6 詳細: The concentration of the protein was 20mg/ml. The precipitant solution in the reservoir was 0.1M sodium acetate/acetic acid buffer of pH 4.6 to 5.0 containing 1.2 to 1.5M sodium chloride. The ...詳細: The concentration of the protein was 20mg/ml. The precipitant solution in the reservoir was 0.1M sodium acetate/acetic acid buffer of pH 4.6 to 5.0 containing 1.2 to 1.5M sodium chloride. The soaking solution was 0.1M sodium acetate/acetic acid buffer at pH 3.5 containing 1.4M NaCl, 25% v/v glycerol (as cryo-protectant) and 2.5M of guanidine hydrochloride.
解像度: 1.75→55.76 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.915 / SU B: 3.421 / SU ML: 0.099 / 交差検証法: THROUGHOUT / ESU R: 0.165 / ESU R Free: 0.143 / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS